Restriction map for
pF1KB5121
##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII AclI AflII AgeI Aor51HI
AseI AsnI Asp700I AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BcgI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsiWI BsmI BsoBI BspHI
BspMI BsrBI BsrDI Bst1107I BstAPI
BstEII BstPI BstXI BstZ17I CelII
CpoI DraI DraIII DrdI EarI
Eco47III EcoNI EcoO65I FauI FbaI
FseI FspI HindIII HpaI KasI
Ksp632I KspI MluI MluNI MscI
NaeI NarI NciI NdeI NgoMIV
NotI NruI PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI SwaI Van91I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 64 enzymes) #
###################################
AccIII 1121 TCCGGA T^CCGGA
AcyI 1084 GACGCC G[GA]^CG[CT]C
AflIII 98 ACATGT A^C[GA][CT]GT
AhdI 1348 GACAAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 71 GTGCAC G^TGCAC
AlwNI 596 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 876 CCTCAGG CC^T[ACGT]AGG
ApaI 172 GGGCCC GGGCC^C
ApaLI 71 GTGCAC G^TGCAC
AscI 1244 GGCGCGCC GG^CGCGCC
AspEI 1348 GACAAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 217 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 172 GGGCCC G[GA]GC[CT]^C
BbvCI 1104 CCTCAGC CCTCAGC(-5/-2)
BciVI 687 GTATCC GTATCC(6/5)
BsaAI 106 TACGTG [CT]AC^GT[GA]
BsaHI 1084 GACGCC G[GA]^CG[CT]C
BsaWI 1121 TCCGGA [AT]^CCGG[AT]
BseAI 1121 TCCGGA T^CCGGA
BsgI 522 CTGCAC GTGCAG(16/14)
BsiEI 157 CGGCCG CG[GA][CT]^CG
BsmBI 1129 GAGACG CGTCTC(1/5)
BsmFI 1356 GTCCC GGGAC(10/14)
Bsp1407I 1416 TGTACA T^GTACA
BspDI 199 ATCGAT AT^CGAT
BspEI 1121 TCCGGA T^CCGGA
BspLU11I 98 ACATGT A^CATGT
BsrFI 1256 ACCGGC [GA]^CCGG[CT]
BsrGI 1416 TGTACA T^GTACA
BssHII 1245 GCGCGC G^CGCGC
BssSI 1152 CACGAG CACGAG(-5/-1)
BstBI 494 TTCGAA TT^CGAA
Bsu36I 876 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1256 ACCGGC [GA]^CCGG[CT]
ClaI 199 ATCGAT AT^CGAT
EaeI 157 CGGCCG [CT]^GGCC[GA]
EagI 157 CGGCCG C^GGCCG
Eam1105I 1348 GACAAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 157 CGGCCG C^GGCCG
Eco52I 157 CGGCCG C^GGCCG
Eco81I 876 CCTCAGG CC^T[ACGT]AGG
EcoRI 1338 GAATTC G^AATTC
EcoRV 500 GATATC GAT^ATC
EcoT22I 809 ATGCAT ATGCA^T
HaeII 1195 AGCGCC [GA]GCGC^[CT]
HgaI 1084 GACGC GACGC(5/10)
Hin1I 1084 GACGCC G[GA]^CG[CT]C
MfeI 635 CAATTG C^AATTG
MroI 1121 TCCGGA T^CCGGA
MspA1I 473 CAGCTG C[AC]G^C[GT]G
MunI 635 CAATTG C^AATTG
NcoI 59 CCATGG C^CATGG
NheI 1021 GCTAGC G^CTAGC
NsiI 809 ATGCAT ATGCA^T
NspV 494 TTCGAA TT^CGAA
PflFI 217 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 815 CTGCAG CTGCA^G
PvuII 473 CAGCTG CAG^CTG
SfcI 815 CTGCAG C^T[GA][CT]AG
SfuI 494 TTCGAA TT^CGAA
SspBI 1416 TGTACA T^GTACA
StuI 1235 AGGCCT AGG^CCT
Tth111I 217 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 209 CCAGCATAGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 351 GGTACC G^GTACC
1266 GGTACC
AccI 181 GTCGAC GT^[AC][GT]AC
1081 GTCGAC
AccII 1231 CGCG CG^CG
1246 CGCG
AcsI 568 GAATTT [GA]^AATT[CT]
1338 GAATTC
ApoI 568 GAATTT [GA]^AATT[CT]
1338 GAATTC
Asp718I 351 GGTACC G^GTACC
1266 GGTACC
AspHI 71 GTGCAC G[AT]GC[AT]^C
1147 GTGCTC
AvaII 1254 GGACC G^G[AT]CC
1355 GGTCC
BanI 351 GGTACC G^G[CT][GA]CC
1266 GGTACC
BsaBI 192 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
919 GATATTTATC
BsaI 883 GAGACC GGTCTC(1/5)
222 GGTCTC
BseRI 1102 CTCCTC GAGGAG(10/8)
584 GAGGAG
BsiHKAI 71 GTGCAC G[AT]GC[AT]^C
1147 GTGCTC
BstUI 1231 CGCG CG^CG
1246 CGCG
DsaI 59 CCATGG C^C[GA][CT]GG
1089 CCACGG
EcoT14I 59 CCATGG C^C[AT][AT]GG
1169 CCAAGG
KpnI 351 GGTACC GGTAC^C
1266 GGTACC
MaeII 107 ACGT A^CGT
185 ACGT
MamI 192 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
919 GATATTTATC
MseI 608 TTAA T^TAA
1391 TTAA
MvnI 1231 CGCG CG^CG
1246 CGCG
SalI 181 GTCGAC G^TCGAC
1081 GTCGAC
StyI 59 CCATGG C^C[AT][AT]GG
1169 CCAAGG
Tru9I 608 TTAA T^TAA
1391 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BfaI BmyI BpmI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MflI MnlI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII