Restriction map for
pF1KB5194
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# NO CUT ENZYMES ( 192 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI AluI AlwI AlwNI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BcnI BfaI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmAI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoT22I
FauI FseI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HindIII HpaI HpaII HphI KasI
KpnI KspI MaeI MaeII MamI
MfeI MflI MluI MluNI MroI
MscI MspA1I MspI MunI MvnI
MwoI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcsI 271 GAATTC [GA]^AATT[CT]
AfaI 457 GTAC GT^AC
Alw44I 183 GTGCAC G^TGCAC
AocI 394 CCTGAGG CC^T[ACGT]AGG
ApaLI 183 GTGCAC G^TGCAC
ApoI 271 GAATTC [GA]^AATT[CT]
Asp700I 304 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 183 GTGCAC G[AT]GC[AT]^C
AvaII 264 GGACC G^G[AT]CC
BclI 377 TGATCA T^GATCA
BmyI 183 GTGCAC G[AGT]GC[ACT]^C
BsiHKAI 183 GTGCAC G[AT]GC[AT]^C
BsmFI 263 GGGAC GGGAC(10/14)
BsmI 150 GAATGC GAATGC(1/-1)
Bsp1286I 183 GTGCAC G[AGT]GC[ACT]^C
BssKI 453 CCAGG ^CC[ACGT]GG
BstNI 453 CCAGG CC^[AT]GG
Bsu36I 394 CCTGAGG CC^T[ACGT]AGG
Cfr13I 264 GGACC G^G[ACGT]CC
DpnII 378 GATC ^GATC
Eco81I 394 CCTGAGG CC^T[ACGT]AGG
EcoRI 271 GAATTC G^AATTC
EcoRII 453 CCAGG ^CC[AT]GG
EcoRV 250 GATATC GAT^ATC
EcoT14I 288 CCAAGG C^C[AT][AT]GG
FbaI 377 TGATCA T^GATCA
HincII 283 GTTGAC GT[CT]^[GA]AC
HindII 283 GTTGAC GT[CT]^[GA]AC
HinfI 402 GATTC G^A[ACGT]TC
MboI 378 GATC ^GATC
MslI 193 CACAAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 453 CCAGG CC^[AT]GG
NdeII 378 GATC ^GATC
NlaIV 263 GGGACC GG[ACGT]^[ACGT]CC
NspI 355 ACATGC [GA]CATG^[CT]
RsaI 457 GTAC GT^AC
Sau3AI 378 GATC ^GATC
Sau96I 264 GGACC G^G[ACGT]CC
ScrFI 453 CCAGG CC^[ACGT]GG
SspI 159 AATATT AAT^ATT
StyI 288 CCAAGG C^C[AT][AT]GG
TfiI 402 GATTC G^A[AT]TC
Tsp45I 73 GTGAC ^GT[GC]AC
XmnI 304 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BbvI 154 GCAGC GCAGC(8/12)
316 GCTGC
BsaJI 288 CCAAGG C^C[ACGT][ACGT]GG
452 CCCAGG
BsiYI 189 CCACCACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
255 CCCTGTTTGGG
BslI 189 CCACCACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
255 CCCTGTTTGGG
BsrI 130 CCAGT ACTGG(1/-1)
471 CCAGT
BstF5I 108 GGATG GGATG(2/0)
198 GGATG
DdeI 327 CTTAG C^T[ACGT]AG
395 CTGAG
EarI 439 GAAGAG CTCTTC(1/4)
481 GAAGAG
Fnu4HI 154 GCAGC GC^[ACGT]GC
316 GCTGC
FokI 108 GGATG GGATG(9/13)
198 GGATG
ItaI 154 GCAGC GC^[ACGT]GC
316 GCTGC
Ksp632I 439 GAAGAG CTCTTC(1/4)
481 GAAGAG
MaeIII 73 GTGAC ^GT[ACGT]AC
352 GTTAC
NlaIII 87 CATG CATG^
356 CATG
TseI 154 GCAGC G^C[AT]GC
316 GCTGC
TspRI 178 CAGTG CA[GC]TG[ACGT][ACGT]^
472 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 6 enzymes) #
#########################################
BseRI MboII MnlI MseI Tru9I
Tsp509I