Restriction map for
pF1KB5260
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI Alw44I
AlwNI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspI AviII AvrII BbrPI BbsI
BcgI BciVI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaWI
BseAI BsiWI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII EagI EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MamI MfeI
MluI MroI MseI MunI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfuI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tru9I Tth111I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AhdI 325 GACGCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 325 GACGCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 383 TGGCCA TGG^CCA
BamHI 77 GGATCC G^GATCC
BbeI 947 GGCGCC GGCGC^C
BclI 531 TGATCA T^GATCA
BfaI 766 CTAG C^TAG
BglI 41 GCCTGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 756 GGTCTC GGTCTC(1/5)
Bsp1407I 311 TGTACA T^GTACA
BspMI 84 GCAGGT ACCTGC(4/8)
BsrGI 311 TGTACA T^GTACA
BsrI 822 CCAGT ACTGG(1/-1)
BstEII 570 GGTGACC G^GT[ACGT]ACC
BstPI 570 GGTGACC G^GT[ACGT]ACC
BstXI 1010 CCACTGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 97 GACTGTATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 797 CCGTGG C^C[GA][CT]GG
Eam1105I 325 GACGCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 409 CTCTTC CTCTTC(1/4)
EcoO65I 570 GGTGACC G^GT[ACGT]ACC
FbaI 531 TGATCA T^GATCA
KasI 947 GGCGCC G^GCGCC
Ksp632I 409 CTCTTC CTCTTC(1/4)
MaeI 766 CTAG C^TAG
MluNI 383 TGGCCA TGG^CCA
MscI 383 TGGCCA TGG^CCA
MspA1I 67 CAGCTG C[AC]G^C[GT]G
NaeI 284 GCCGGC GCC^GGC
NarI 947 GGCGCC GG^CGCC
NgoMIV 284 GCCGGC G^CCGGC
PstI 907 CTGCAG CTGCA^G
PvuII 67 CAGCTG CAG^CTG
SapI 408 GCTCTTC GCTCTTC(1/4)
SfcI 907 CTGCAG C^T[GA][CT]AG
SfoI 947 GGCGCC GGC^GCC
SgrAI 283 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 311 TGTACA T^GTACA
TfiI 351 GATTC G^A[AT]TC
Tsp509I 843 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 200 CGCG CG^CG
897 CGCG
AcyI 325 GACGCC G[GA]^CG[CT]C
947 GGCGCC
ApaI 1027 GGGCCC GGGCC^C
1078 GGGCCC
AspHI 154 GAGCAC G[AT]GC[AT]^C
406 GTGCTC
AvaI 1001 CCCGGG C^[CT]CG[GA]G
1022 CCCGGG
BbvCI 747 CCTCAGC CCTCAGC(-5/-2)
717 GCTGAGG
BsaHI 325 GACGCC G[GA]^CG[CT]C
947 GGCGCC
BsgI 70 CTGCAC GTGCAG(16/14)
64 GTGCAG
BsiEI 638 CGACCG CG[GA][CT]^CG
678 CGACCG
BsiHKAI 154 GAGCAC G[AT]GC[AT]^C
406 GTGCTC
BsoBI 1001 CCCGGG C^[CT]CG[GA]G
1022 CCCGGG
BstUI 200 CGCG CG^CG
897 CGCG
BstYI 77 GGATCC [GA]^GATC[CT]
1091 AGATCC
EaeI 383 TGGCCA [CT]^GGCC[GA]
1007 CGGCCA
EcoNI 335 CCTACGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
747 CCTCAGCAAGG
EcoT14I 356 CCAAGG C^C[AT][AT]GG
386 CCAAGG
Hin1I 325 GACGCC G[GA]^CG[CT]C
947 GGCGCC
MflI 77 GGATCC [GA]^GATC[CT]
1091 AGATCC
MvnI 200 CGCG CG^CG
897 CGCG
PleI 226 GACTC GAGTC(4/5)
769 GAGTC
PpuMI 86 AGGTCCT [GA]G^G[AT]CC[CT]
954 GGGACCC
SmaI 1001 CCCGGG CCC^GGG
1022 CCCGGG
StyI 356 CCAAGG C^C[AT][AT]GG
386 CCAAGG
TspRI 983 CACTG CA[GC]TG[ACGT][ACGT]^
1011 CACTG
XhoII 77 GGATCC [GA]^GATC[CT]
1091 AGATCC
XmaCI 1001 CCCGGG C^CCGGG
1022 CCCGGG
XmaI 1001 CCCGGG C^CCGGG
1022 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaAI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrFI
BssKI BstF5I BstNI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI TaqI
TseI Tsp45I TthHB8I XcmI