Restriction map for
pF1KB5300
##################################
# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AviII BamHI
BbeI BbrPI BbsI BcgI BciVI
BclI BglII BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CfoI ClaI
CpoI DraI DraII DraIII DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FspI HaeII HhaI
Hin1I HinP1I HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MslI MunI MvnI NarI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SwaI TfiI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccI 67 GTCGAC GT^[AC][GT]AC
AcsI 142 AAATTC [GA]^AATT[CT]
AfaI 225 GTAC GT^AC
AflII 733 CTTAAG C^TTAAG
AhdI 802 GACCTAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 660 GATCC GGATC(4/5)
ApoI 142 AAATTC [GA]^AATT[CT]
AspEI 802 GACCTAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 189 CCTAGG C^CTAGG
BanI 1189 GGTGCC G^G[CT][GA]CC
BbvCI 973 GCTGAGG CCTCAGC(-5/-2)
BfrI 733 CTTAAG C^TTAAG
BlnI 189 CCTAGG C^CTAGG
BlpI 991 GCTAAGC GC^T[ACGT]AGC
BpmI 355 CTGGAG CTGGAG(16/14)
Bpu1102I 991 GCTAAGC GC^T[ACGT]AGC
BsmAI 1121 GAGAC GTCTC(1/5)
BsmFI 130 GTCCC GGGAC(10/14)
BsmI 882 GAATGC GAATGC(1/-1)
BspMI 921 ACCTGC ACCTGC(4/8)
BsrFI 194 GCCGGC [GA]^CCGG[CT]
BstYI 659 AGATCC [GA]^GATC[CT]
CelII 991 GCTAAGC GC^T[ACGT]AGC
Cfr10I 194 GCCGGC [GA]^CCGG[CT]
DpnII 660 GATC ^GATC
DsaI 524 CCATGG C^C[GA][CT]GG
Eam1105I 802 GACCTAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 778 GAAGAG CTCTTC(1/4)
FauI 51 GCGGG CCCGC(4/6)
FseI 193 GGCCGGCC GGCCGG^CC
HgaI 21 GACGC GACGC(5/10)
Ksp632I 778 GAAGAG CTCTTC(1/4)
MaeII 38 ACGT A^CGT
MboI 660 GATC ^GATC
MflI 659 AGATCC [GA]^GATC[CT]
MspA1I 332 CAGCTG C[AC]G^C[GT]G
NaeI 194 GCCGGC GCC^GGC
NcoI 524 CCATGG C^CATGG
NdeII 660 GATC ^GATC
NgoMIV 194 GCCGGC G^CCGGC
PflMI 331 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 943 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 332 CAGCTG CAG^CTG
RsaI 225 GTAC GT^AC
SacI 1201 GAGCTC GAGCT^C
SalI 67 GTCGAC G^TCGAC
Sau3AI 660 GATC ^GATC
ScaI 224 AGTACT AGT^ACT
SfcI 245 CTGTAG C^T[GA][CT]AG
SmaI 1046 CCCGGG CCC^GGG
SmlI 733 CTTAAG C^T[CT][GA]AG
SspI 1246 AATATT AAT^ATT
StuI 596 AGGCCT AGG^CCT
TaqI 68 TCGA T^CGA
Tsp509I 143 AATT ^AATT
TthHB8I 68 TCGA T^CGA
Van91I 331 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 659 AGATCC [GA]^GATC[CT]
XmaCI 1046 CCCGGG C^CCGGG
XmaI 1046 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwNI 239 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
332 CAGCTGCTG
AspI 685 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
853 GACTTTGTC
AvaI 986 CTCGGG C^[CT]CG[GA]G
1046 CCCGGG
AvaII 949 GGTCC G^G[AT]CC
1012 GGTCC
BalI 626 TGGCCA TGG^CCA
894 TGGCCA
BcnI 1046 CCCGG CC^[GC]GG
1047 CCGGG
BglI 185 GCCACCTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
901 GCCACTGTGGC
BsoBI 986 CTCGGG C^[CT]CG[GA]G
1046 CCCGGG
EaeI 626 TGGCCA [CT]^GGCC[GA]
894 TGGCCA
HapII 195 CCGG C^CGG
1047 CCGG
HincII 67 GTCGAC GT[CT]^[GA]AC
850 GTTGAC
HindII 67 GTCGAC GT[CT]^[GA]AC
850 GTTGAC
HindIII 443 AAGCTT A^AGCTT
1037 AAGCTT
HinfI 65 GAGTC G^A[ACGT]TC
533 GAGTC
HpaII 195 CCGG C^CGG
1047 CCGG
HphI 1077 GGTGA GGTGA(8/7)
1103 TCACC
MaeIII 859 GTCAC ^GT[ACGT]AC
982 GTCAC
MluNI 626 TGGCCA TGG^CCA
894 TGGCCA
MscI 626 TGGCCA TGG^CCA
894 TGGCCA
MspI 195 CCGG C^CGG
1047 CCGG
NciI 1046 CCCGG CC^[GC]GG
1047 CCGGG
PflFI 685 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
853 GACTTTGTC
PleI 65 GAGTC GAGTC(4/5)
533 GAGTC
Tsp45I 859 GTCAC ^GT[GC]AC
982 GTCAC
Tth111I 685 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
853 GACTTTGTC
XcmI 1055 CCAAAGCTCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1170 CCATGTGAGCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI AspHI BanII BbvI
BfaI BmyI BsaJI BsiHKAI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI EcoRII
EcoT14I Fnu4HI FokI HaeIII ItaI
MaeI MboII MnlI MseI MvaI
MwoI NlaIII NlaIV Sau96I ScrFI
SfaNI StyI Tru9I TseI TspRI