Restriction map for
pF1KB5343
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
Acc65I AcsI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvrII BamHI
BanII BbeI BbvCI BcgI BclI
BfaI BfrI BglII BlnI BsaBI
BsaI BsiHKAI BsiWI BsmAI BsmBI
Bsp1407I BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI HgaI KasI KpnI MaeI
MamI MfeI MflI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PflFI
PinAI PmeI PshBI PvuI RcaI
RsrII SacI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI TfiI Tsp509I Tth111I
XbaI XcmI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 1099 GACGTC GACGT^C
AccI 1132 GTCGAC GT^[AC][GT]AC
AclI 186 AACGTT AA^CGTT
AcyI 1099 GACGTC G[GA]^CG[CT]C
AflIII 794 ACGCGT A^C[GA][CT]GT
AlwI 602 GGATC GGATC(4/5)
Asp700I 990 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 748 CTCGAG C^[CT]CG[GA]G
AviII 533 TGCGCA TGC^GCA
BbrPI 460 CACGTG CAC^GTG
BciVI 369 GTATCC GTATCC(6/5)
BlpI 84 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 84 GCTGAGC GC^T[ACGT]AGC
BsaHI 1099 GACGTC G[GA]^CG[CT]C
BsiEI 1134 CGACCG CG[GA][CT]^CG
BsmI 113 GCATTC GAATGC(1/-1)
BsoBI 748 CTCGAG C^[CT]CG[GA]G
BspDI 382 ATCGAT AT^CGAT
BspMI 1166 GCAGGT ACCTGC(4/8)
CelII 84 GCTGAGC GC^T[ACGT]AGC
ClaI 382 ATCGAT AT^CGAT
DraI 318 TTTAAA TTT^AAA
DrdI 646 GACTACAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 531 CCTGCGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 533 TGCGCA TGC^GCA
HaeII 1598 AGCGCC [GA]GCGC^[CT]
Hin1I 1099 GACGTC G[GA]^CG[CT]C
HindIII 973 AAGCTT A^AGCTT
HpaI 160 GTTAAC GTT^AAC
KspI 442 CCGCGG CCGC^GG
MluI 794 ACGCGT A^CGCGT
MslI 1608 CATTATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 485 GCCGGC GCC^GGC
NgoMIV 485 GCCGGC G^CCGGC
PaeR7I 748 CTCGAG C^TCGAG
PflMI 593 CCATCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 460 CACGTG CAC^GTG
PmlI 460 CACGTG CAC^GTG
PshAI 1152 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 186 AACGTT AA^CGTT
PstI 1333 CTGCAG CTGCA^G
SacII 442 CCGCGG CCGC^GG
SalI 1132 GTCGAC G^TCGAC
ScaI 1235 AGTACT AGT^ACT
StuI 6 AGGCCT AGG^CCT
Van91I 593 CCATCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 748 CTCGAG C^TCGAG
XmnI 990 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 266 TCCGGA T^CCGGA
275 TCCGGA
AlwNI 1451 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1564 CAGCGGCTG
AvaII 396 GGACC G^G[AT]CC
1347 GGACC
BalI 659 TGGCCA TGG^CCA
1604 TGGCCA
BbsI 931 GAAGAC GAAGAC(2/6)
541 GTCTTC
BpuAI 931 GAAGAC GAAGAC(2/6)
541 GTCTTC
BsaAI 460 CACGTG [CT]AC^GT[GA]
1174 TACGTG
BsaWI 266 TCCGGA [AT]^CCGG[AT]
275 TCCGGA
BseAI 266 TCCGGA T^CCGGA
275 TCCGGA
BsmFI 395 GGGAC GGGAC(10/14)
1067 GGGAC
BspEI 266 TCCGGA T^CCGGA
275 TCCGGA
BstF5I 891 GGATG GGATG(2/0)
1508 GGATG
DpnII 603 GATC ^GATC
1650 GATC
DsaI 442 CCGCGG C^C[GA][CT]GG
1226 CCGTGG
FokI 891 GGATG GGATG(9/13)
1508 GGATG
MaeIII 1102 GTCAC ^GT[ACGT]AC
1279 GTGAC
MboI 603 GATC ^GATC
1650 GATC
MluNI 659 TGGCCA TGG^CCA
1604 TGGCCA
MroI 266 TCCGGA T^CCGGA
275 TCCGGA
MscI 659 TGGCCA TGG^CCA
1604 TGGCCA
MseI 161 TTAA T^TAA
319 TTAA
NdeII 603 GATC ^GATC
1650 GATC
PpuMI 395 GGGACCT [GA]G^G[AT]CC[CT]
1346 AGGACCC
SapI 291 GAAGAGC GCTCTTC(1/4)
1498 GAAGAGC
Sau3AI 603 GATC ^GATC
1650 GATC
SmlI 10 CTTGAG C^T[CT][GA]AG
748 CTCGAG
Tru9I 161 TTAA T^TAA
319 TTAA
Tsp45I 1102 GTCAC ^GT[GC]AC
1279 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AfaI AluI BanI
BbvI BcnI BglI BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
Bsp1286I BsrFI BsrI BssKI BstNI
BstUI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DraII EaeI EarI
EcoO109I EcoRII FauI Fnu4HI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeII MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV PleI PvuII RsaI Sau96I
ScrFI SfaNI SfcI TaqI TseI
TspRI TthHB8I