Restriction map for
pF1KB5469
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# NO CUT ENZYMES ( 192 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmFI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstNI BstPI
BstXI BstZ17I Bsu36I Cac8I CelII
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRII
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MluNI MroI MscI MspA1I
MunI MvaI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TfiI Tsp45I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 124 GTAGAC GT^[AC][GT]AC
AccII 132 CGCG CG^CG
AfaI 426 GTAC GT^AC
AlwNI 441 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
BsaJI 174 CCGAGG C^C[ACGT][ACGT]GG
BsmAI 418 GAGAC GTCTC(1/5)
BsmBI 418 GAGACG CGTCTC(1/5)
BsmI 203 GCATTC GAATGC(1/-1)
BspMI 325 ACCTGC ACCTGC(4/8)
BsrDI 63 CATTGC GCAATG(2/0)
BstF5I 276 GGATG GGATG(2/0)
BstUI 132 CGCG CG^CG
BstYI 387 AGATCC [GA]^GATC[CT]
CfoI 131 GCGC GCG^C
Cfr13I 468 GGCCC G^G[ACGT]CC
EarI 192 GAAGAG CTCTTC(1/4)
FokI 276 GGATG GGATG(9/13)
HaeIII 468 GGCC GG^CC
HhaI 131 GCGC GCG^C
HinP1I 131 GCGC G^CGC
HinfI 406 GACTC G^A[ACGT]TC
HphI 58 TCACC GGTGA(8/7)
Ksp632I 192 GAAGAG CTCTTC(1/4)
MaeII 218 ACGT A^CGT
MflI 387 AGATCC [GA]^GATC[CT]
MseI 320 TTAA T^TAA
MslI 204 CATTCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 132 CGCG CG^CG
MwoI 4 GCGGAACAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 158 GGAACC GG[ACGT]^[ACGT]CC
PleI 406 GACTC GAGTC(4/5)
PstI 447 CTGCAG CTGCA^G
RsaI 426 GTAC GT^AC
Sau96I 468 GGCCC G^G[ACGT]CC
SfcI 447 CTGCAG C^T[GA][CT]AG
Tru9I 320 TTAA T^TAA
TspRI 433 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 387 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 4 GCGG CCGC(-3/-1)
133 GCGG
AlwI 361 GATCC GGATC(4/5)
388 GATCC
BbvI 185 GCTGC GCAGC(8/12)
446 GCTGC
BcnI 143 CCGGG CC^[GC]GG
224 CCCGG
BsiYI 391 CCCTATTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
392 CCTATTATGGG
BslI 391 CCCTATTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
392 CCTATTATGGG
BssKI 143 CCGGG ^CC[ACGT]GG
224 CCCGG
DdeI 108 CTCAG C^T[ACGT]AG
289 CTGAG
HapII 143 CCGG C^CGG
225 CCGG
HpaII 143 CCGG C^CGG
225 CCGG
MaeIII 44 GTAAC ^GT[ACGT]AC
91 GTAAC
MspI 143 CCGG C^CGG
225 CCGG
NciI 143 CCGGG CC^[GC]GG
224 CCCGG
NlaIII 189 CATG CATG^
210 CATG
ScrFI 143 CCGGG CC^[ACGT]GG
224 CCCGG
TaqI 54 TCGA T^CGA
315 TCGA
TseI 185 GCTGC G^C[AT]GC
446 GCTGC
Tsp509I 115 AATT ^AATT
339 AATT
TthHB8I 54 TCGA T^CGA
315 TCGA
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# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
#########################################
AluI DpnII Fnu4HI ItaI MboI
MboII MnlI NdeII Sau3AI