Restriction map for pF1KB5511
##################################
# NO CUT ENZYMES (  102 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        BamHI       BbeI        BbrPI       BcgI        
BfrI        BseAI       BsiEI       BsiWI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrGI       
BssHII      Bst1107I    BstAPI      BstEII      BstPI       
BstZ17I     ClaI        CpoI        DraI        DrdI        
EagI        Eam1105I    EclXI       Eco52I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MfeI        MluI        MroI        MseI        
MunI        NarI        NdeI        NheI        NotI        
NruI        NsiI        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tru9I       Tth111I     
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AatII         207  GACGTC               GACGT^C
AccII         773  CGCG                 CG^CG
AcsI         1840  AAATTC               [GA]^AATT[CT]
AlwNI         622  CAGAACCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1054  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       134  AGCGCT               AGC^GCT
ApoI         1840  AAATTC               [GA]^AATT[CT]
Asp700I       342  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII         821  TGCGCA               TGC^GCA
AvrII         177  CCTAGG               C^CTAGG
BanI         2146  GGCACC               G^G[CT][GA]CC
BbsI          373  GTCTTC               GAAGAC(2/6)
BciVI         423  GTATCC               GTATCC(6/5)
BclI         1367  TGATCA               T^GATCA
BglI          827  GCCTCAAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI          177  CCTAGG               C^CTAGG
BlpI          137  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      137  GCTCAGC              GC^T[ACGT]AGC
BpuAI         373  GTCTTC               GAAGAC(2/6)
BsaAI        1300  TACGTG               [CT]AC^GT[GA]
BsaBI         585  GATCAATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         426  TCCGGT               [AT]^CCGG[AT]
BsmBI        1645  GAGACG               CGTCTC(1/5)
BsmI         1071  GAATGC               GAATGC(1/-1)
BsrBI        1825  GAGCGG               CCGCTC(-3/-3)
BssSI         973  CACGAG               CACGAG(-5/-1)
BstBI         787  TTCGAA               TT^CGAA
BstUI         773  CGCG                 CG^CG
Bsu36I       1054  CCTGAGG              CC^T[ACGT]AGG
CelII         137  GCTCAGC              GC^T[ACGT]AGC
DraIII        801  CACCGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III      134  AGCGCT               AGC^GCT
Eco81I       1054  CCTGAGG              CC^T[ACGT]AGG
FbaI         1367  TGATCA               T^GATCA
FspI          821  TGCGCA               TGC^GCA
MaeIII        809  GTGAC                ^GT[ACGT]AC
MamI          585  GATCAATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI          773  CGCG                 CG^CG
NaeI         1106  GCCGGC               GCC^GGC
NcoI          720  CCATGG               C^CATGG
NgoMIV       1106  GCCGGC               G^CCGGC
NspI          950  ACATGC               [GA]CATG^[CT]
NspV          787  TTCGAA               TT^CGAA
PflMI         621  CCAGAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfuI          787  TTCGAA               TT^CGAA
SmaI          329  CCCGGG               CCC^GGG
TfiI          119  GATTC                G^A[AT]TC
Tsp45I        809  GTGAC                ^GT[GC]AC
Tsp509I      1841  AATT                 ^AATT
Van91I        621  CCAGAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         329  CCCGGG               C^CCGGG
XmaI          329  CCCGGG               C^CCGGG
XmnI          342  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AfaI         1599  GTAC                 GT^AC
             1854  GTAC                
AvaI          329  CCCGGG               C^[CT]CG[GA]G
             1579  CCCGAG              
BbvCI         489  CCTCAGC              CCTCAGC(-5/-2)
              382  GCTGAGG             
BfaI          178  CTAG                 C^TAG
             1522  CTAG                
BglII         554  AGATCT               A^GATCT
             1407  AGATCT              
BseRI         385  GAGGAG               GAGGAG(10/8)
             1711  GAGGAG              
BsmFI         205  GGGAC                GGGAC(10/14)
              903  GTCCC               
BsoBI         329  CCCGGG               C^[CT]CG[GA]G
             1579  CCCGAG              
BspMI        1544  ACCTGC               ACCTGC(4/8)
              214  GCAGGT              
BsrDI        1209  CATTGC               GCAATG(2/0)
             1857  CATTGC              
BsrFI        1106  GCCGGC               [GA]^CCGG[CT]
             1493  ACCGGC              
Cfr10I       1106  GCCGGC               [GA]^CCGG[CT]
             1493  ACCGGC              
DsaI          720  CCATGG               C^C[GA][CT]GG
              857  CCACGG              
EcoNI         177  CCTAGGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1557  CCTCTGGGAGG         
HaeII         134  AGCGCT               [GA]GCGC^[CT]
              168  GGCGCT              
HgaI           61  GACGC                GACGC(5/10)
             1647  GACGC               
MaeI          178  CTAG                 C^TAG
             1522  CTAG                
PleI         2108  GACTC                GAGTC(4/5)
             2009  GAGTC               
PstI          742  CTGCAG               CTGCA^G
             1396  CTGCAG              
PvuII         538  CAGCTG               CAG^CTG
             1119  CAGCTG              
RsaI         1599  GTAC                 GT^AC
             1854  GTAC                
SacI          334  GAGCTC               GAGCT^C
              388  GAGCTC              
SfcI          742  CTGCAG               C^T[GA][CT]AG
             1396  CTGCAG              
SmlI          655  CTCAAG               C^T[CT][GA]AG
              829  CTCAAG              


#########################################
# 3 OR MORE CUT ENZYMES (   79 enzymes) #
#########################################

AciI        AcyI        AluI        AlwI        ApaI        
AspHI       AvaII       BalI        BanII       BbvI        
BcnI        BmyI        BpmI        BsaHI       BsaI        
BsaJI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstXI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeII       MboI        MboII       
MflI        MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       Sau3AI      Sau96I      
ScrFI       SfaNI       StyI        TaqI        TseI        
TspRI       TthHB8I     XcmI        XhoII       
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