################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp718I AspEI AspI BamHI BbeI BbrPI BcgI BfrI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII Bst1107I BstAPI BstEII BstPI BstZ17I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FseI HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MroI MseI MunI NarI NdeI NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AatII 207 GACGTC GACGT^C AccII 773 CGCG CG^CG AcsI 1840 AAATTC [GA]^AATT[CT] AlwNI 622 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1054 CCTGAGG CC^T[ACGT]AGG Aor51HI 134 AGCGCT AGC^GCT ApoI 1840 AAATTC [GA]^AATT[CT] Asp700I 342 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 821 TGCGCA TGC^GCA AvrII 177 CCTAGG C^CTAGG BanI 2146 GGCACC G^G[CT][GA]CC BbsI 373 GTCTTC GAAGAC(2/6) BciVI 423 GTATCC GTATCC(6/5) BclI 1367 TGATCA T^GATCA BglI 827 GCCTCAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 177 CCTAGG C^CTAGG BlpI 137 GCTCAGC GC^T[ACGT]AGC Bpu1102I 137 GCTCAGC GC^T[ACGT]AGC BpuAI 373 GTCTTC GAAGAC(2/6) BsaAI 1300 TACGTG [CT]AC^GT[GA] BsaBI 585 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 426 TCCGGT [AT]^CCGG[AT] BsmBI 1645 GAGACG CGTCTC(1/5) BsmI 1071 GAATGC GAATGC(1/-1) BsrBI 1825 GAGCGG CCGCTC(-3/-3) BssSI 973 CACGAG CACGAG(-5/-1) BstBI 787 TTCGAA TT^CGAA BstUI 773 CGCG CG^CG Bsu36I 1054 CCTGAGG CC^T[ACGT]AGG CelII 137 GCTCAGC GC^T[ACGT]AGC DraIII 801 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 134 AGCGCT AGC^GCT Eco81I 1054 CCTGAGG CC^T[ACGT]AGG FbaI 1367 TGATCA T^GATCA FspI 821 TGCGCA TGC^GCA MaeIII 809 GTGAC ^GT[ACGT]AC MamI 585 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 773 CGCG CG^CG NaeI 1106 GCCGGC GCC^GGC NcoI 720 CCATGG C^CATGG NgoMIV 1106 GCCGGC G^CCGGC NspI 950 ACATGC [GA]CATG^[CT] NspV 787 TTCGAA TT^CGAA PflMI 621 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SfuI 787 TTCGAA TT^CGAA SmaI 329 CCCGGG CCC^GGG TfiI 119 GATTC G^A[AT]TC Tsp45I 809 GTGAC ^GT[GC]AC Tsp509I 1841 AATT ^AATT Van91I 621 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 329 CCCGGG C^CCGGG XmaI 329 CCCGGG C^CCGGG XmnI 342 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AfaI 1599 GTAC GT^AC 1854 GTAC AvaI 329 CCCGGG C^[CT]CG[GA]G 1579 CCCGAG BbvCI 489 CCTCAGC CCTCAGC(-5/-2) 382 GCTGAGG BfaI 178 CTAG C^TAG 1522 CTAG BglII 554 AGATCT A^GATCT 1407 AGATCT BseRI 385 GAGGAG GAGGAG(10/8) 1711 GAGGAG BsmFI 205 GGGAC GGGAC(10/14) 903 GTCCC BsoBI 329 CCCGGG C^[CT]CG[GA]G 1579 CCCGAG BspMI 1544 ACCTGC ACCTGC(4/8) 214 GCAGGT BsrDI 1209 CATTGC GCAATG(2/0) 1857 CATTGC BsrFI 1106 GCCGGC [GA]^CCGG[CT] 1493 ACCGGC Cfr10I 1106 GCCGGC [GA]^CCGG[CT] 1493 ACCGGC DsaI 720 CCATGG C^C[GA][CT]GG 857 CCACGG EcoNI 177 CCTAGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1557 CCTCTGGGAGG HaeII 134 AGCGCT [GA]GCGC^[CT] 168 GGCGCT HgaI 61 GACGC GACGC(5/10) 1647 GACGC MaeI 178 CTAG C^TAG 1522 CTAG PleI 2108 GACTC GAGTC(4/5) 2009 GAGTC PstI 742 CTGCAG CTGCA^G 1396 CTGCAG PvuII 538 CAGCTG CAG^CTG 1119 CAGCTG RsaI 1599 GTAC GT^AC 1854 GTAC SacI 334 GAGCTC GAGCT^C 388 GAGCTC SfcI 742 CTGCAG C^T[GA][CT]AG 1396 CTGCAG SmlI 655 CTCAAG C^T[CT][GA]AG 829 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AciI AcyI AluI AlwI ApaI AspHI AvaII BalI BanII BbvI BcnI BmyI BpmI BsaHI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI StyI TaqI TseI TspRI TthHB8I XcmI XhoII