Restriction map for
pF1KB5513
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# NO CUT ENZYMES ( 98 enzymes) #
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AatII AccI AccIII AclI AcyI
AflII AgeI AhdI Alw44I ApaLI
AscI AseI AsnI AspEI AspI
AviII BalI BbeI BbrPI BcgI
BciVI BfrI BlpI Bpu1102I BsaAI
BsaHI BsaI BseAI BsiWI BsmBI
Bsp1407I BspEI BspHI BspLU11I BsrFI
BsrGI BssHII Bst1107I BstXI BstZ17I
CelII Cfr10I CpoI DraI DraIII
DrdI Eam1105I EcoT22I FseI FspI
Hin1I HindIII HpaI KasI MfeI
MluNI MroI MscI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI SexAI SfiI
SfoI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XbaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 1858 GGTACC G^GTACC
AflIII 785 ACGCGT A^C[GA][CT]GT
AocI 993 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1379 AGCGCT AGC^GCT
ApaI 190 GGGCCC GGGCC^C
Asp718I 1858 GGTACC G^GTACC
AvrII 303 CCTAGG C^CTAGG
BamHI 2037 GGATCC G^GATCC
BbsI 613 GAAGAC GAAGAC(2/6)
BbvCI 1341 CCTCAGC CCTCAGC(-5/-2)
BclI 1163 TGATCA T^GATCA
BlnI 303 CCTAGG C^CTAGG
BpuAI 613 GAAGAC GAAGAC(2/6)
BsaBI 843 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1212 CTGCAC GTGCAG(16/14)
BsiEI 41 CGGCCG CG[GA][CT]^CG
BsmI 504 GCATTC GAATGC(1/-1)
BspDI 1969 ATCGAT AT^CGAT
BspMI 1900 GCAGGT ACCTGC(4/8)
BsrBI 1206 GAGCGG CCGCTC(-3/-3)
BstAPI 325 GCAGCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 795 GGTCACC G^GT[ACGT]ACC
BstPI 795 GGTCACC G^GT[ACGT]ACC
Bsu36I 993 CCTGAGG CC^T[ACGT]AGG
ClaI 1969 ATCGAT AT^CGAT
EagI 41 CGGCCG C^GGCCG
EclXI 41 CGGCCG C^GGCCG
Eco47III 1379 AGCGCT AGC^GCT
Eco52I 41 CGGCCG C^GGCCG
Eco81I 993 CCTGAGG CC^T[ACGT]AGG
EcoO65I 795 GGTCACC G^GT[ACGT]ACC
EcoRV 1696 GATATC GAT^ATC
FbaI 1163 TGATCA T^GATCA
HgaI 129 GACGC GACGC(5/10)
KpnI 1858 GGTACC GGTAC^C
MamI 843 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 785 ACGCGT A^CGCGT
PaeR7I 365 CTCGAG C^TCGAG
PpuMI 140 AGGACCC [GA]G^G[AT]CC[CT]
PstI 1657 CTGCAG CTGCA^G
PvuII 2032 CAGCTG CAG^CTG
SacI 2062 GAGCTC GAGCT^C
SapI 132 GCTCTTC GCTCTTC(1/4)
ScaI 2094 AGTACT AGT^ACT
SmaI 209 CCCGGG CCC^GGG
SmlI 365 CTCGAG C^T[CT][GA]AG
XcmI 255 CCAGTTTATCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 365 CTCGAG C^TCGAG
XmaCI 209 CCCGGG C^CCGGG
XmaI 209 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Asp700I 1134 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1782 GAAGGAGTTC
AspHI 1411 GTGCTC G[AT]GC[AT]^C
2062 GAGCTC
AvaII 30 GGACC G^G[AT]CC
141 GGACC
BanI 592 GGTGCC G^G[CT][GA]CC
1858 GGTACC
BglI 16 GCCAAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1615 GCCCAGTTGGC
BsaWI 622 ACCGGA [AT]^CCGG[AT]
799 ACCGGA
BsiHKAI 1411 GTGCTC G[AT]GC[AT]^C
2062 GAGCTC
BsmAI 1605 GAGAC GTCTC(1/5)
1723 GAGAC
BsmFI 127 GGGAC GGGAC(10/14)
373 GTCCC
BsrDI 330 CATTGC GCAATG(2/0)
630 CATTGC
BssSI 61 CACGAG CACGAG(-5/-1)
1450 CTCGTG
BstBI 610 TTCGAA TT^CGAA
1465 TTCGAA
EarI 133 CTCTTC CTCTTC(1/4)
1561 GAAGAG
EcoNI 81 CCTCAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1401 CCTCCGGGAGG
EcoRI 967 GAATTC G^AATTC
1866 GAATTC
Ksp632I 133 CTCTTC CTCTTC(1/4)
1561 GAAGAG
KspI 740 CCGCGG CCGC^GG
1109 CCGCGG
MaeII 760 ACGT A^CGT
1844 ACGT
MseI 1318 TTAA T^TAA
2027 TTAA
NspV 610 TTCGAA TT^CGAA
1465 TTCGAA
SacII 740 CCGCGG CCGC^GG
1109 CCGCGG
SfuI 610 TTCGAA TT^CGAA
1465 TTCGAA
Tru9I 1318 TTAA T^TAA
2027 TTAA
XmnI 1134 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1782 GAAGGAGTTC
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# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AvaI BanII
BbvI BcnI BfaI BglII BmyI
BpmI BsaJI BseRI BsiYI BslI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII