Restriction map for
pF1KB5584
##################################
# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanII BbeI BbrPI BcgI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsoBI BspDI
BspEI BsrBI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FauI FseI FspI
HgaI Hin1I HindIII HpaI KasI
KspI MamI MflI MluI MroI
MspA1I MvnI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SwaI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 1616 GGTACC G^GTACC
AciI 61 CCGC CCGC(-3/-1)
AflIII 228 ACATGT A^C[GA][CT]GT
Asp718I 1616 GGTACC G^GTACC
BalI 1676 TGGCCA TGG^CCA
BanI 1616 GGTACC G^G[CT][GA]CC
BbvCI 1035 CCTCAGC CCTCAGC(-5/-2)
BclI 339 TGATCA T^GATCA
BcnI 259 CCCGG CC^[GC]GG
BmyI 1351 GGGCAC G[AGT]GC[ACT]^C
BpmI 1715 CTGGAG CTGGAG(16/14)
BsaI 509 GAGACC GGTCTC(1/5)
BsaWI 1813 TCCGGT [AT]^CCGG[AT]
BseRI 850 GAGGAG GAGGAG(10/8)
BsgI 1541 GTGCAG GTGCAG(16/14)
Bsp1286I 1351 GGGCAC G[AGT]GC[ACT]^C
Bsp1407I 1758 TGTACA T^GTACA
BspLU11I 228 ACATGT A^CATGT
BspMI 982 GCAGGT ACCTGC(4/8)
BsrDI 931 GCAATG GCAATG(2/0)
BsrFI 238 GCCGGT [GA]^CCGG[CT]
BsrGI 1758 TGTACA T^GTACA
BssSI 1313 CTCGTG CACGAG(-5/-1)
BstAPI 1462 GCAGAAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 1029 CATCC GGATG(2/0)
CfoI 1155 GCGC GCG^C
Cfr10I 238 GCCGGT [GA]^CCGG[CT]
DsaI 276 CCATGG C^C[GA][CT]GG
EaeI 1676 TGGCCA [CT]^GGCC[GA]
FbaI 339 TGATCA T^GATCA
FokI 1029 CATCC GGATG(9/13)
HaeII 1154 GGCGCT [GA]GCGC^[CT]
HhaI 1155 GCGC GCG^C
HinP1I 1155 GCGC G^CGC
HincII 799 GTCAAC GT[CT]^[GA]AC
HindII 799 GTCAAC GT[CT]^[GA]AC
KpnI 1616 GGTACC GGTAC^C
MaeIII 1878 GTTAC ^GT[ACGT]AC
MfeI 877 CAATTG C^AATTG
MluNI 1676 TGGCCA TGG^CCA
MscI 1676 TGGCCA TGG^CCA
MunI 877 CAATTG C^AATTG
NciI 259 CCCGG CC^[GC]GG
NcoI 276 CCATGG C^CATGG
PstI 980 CTGCAG CTGCA^G
SmlI 90 CTTGAG C^T[CT][GA]AG
SspBI 1758 TGTACA T^GTACA
SspI 1793 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BbsI 751 GAAGAC GAAGAC(2/6)
265 GTCTTC
BciVI 38 GGATAC GTATCC(6/5)
693 GGATAC
BpuAI 751 GAAGAC GAAGAC(2/6)
265 GTCTTC
BsaAI 500 TACGTG [CT]AC^GT[GA]
1472 CACGTA
BsmI 157 GCATTC GAATGC(1/-1)
992 GCATTC
BstNI 54 CCTGG CC^[AT]GG
1188 CCTGG
Cac8I 1381 GCCTGC GC[ACGT]^[ACGT]GC
1566 GCAGGC
DraII 1052 GGGACCT [GA]G^G[ACGT]CC[CT]
1515 AGGTCCT
DraIII 141 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
1840 CACTGGGTG
EarI 177 CTCTTC CTCTTC(1/4)
304 GAAGAG
EcoO109I 1052 GGGACCT [GA]G^G[ACGT]CC[CT]
1515 AGGTCCT
EcoRI 724 GAATTC G^AATTC
774 GAATTC
EcoRII 54 CCTGG ^CC[AT]GG
1188 CCTGG
Ksp632I 177 CTCTTC CTCTTC(1/4)
304 GAAGAG
MvaI 54 CCTGG CC^[AT]GG
1188 CCTGG
NspI 228 ACATGT [GA]CATG^[CT]
567 ACATGC
PpuMI 1052 GGGACCT [GA]G^G[AT]CC[CT]
1515 AGGTCCT
StuI 350 AGGCCT AGG^CCT
819 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BbvI BfaI BsaJI BsiYI
BslI BsmAI BsmFI BspHI BsrI
BssKI Cfr13I DdeI DpnII EcoT14I
Fnu4HI HaeIII HapII HinfI HpaII
HphI ItaI MaeI MaeII MboI
MboII MnlI MseI MslI MspI
MwoI NdeI NdeII NlaIII NlaIV
PleI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I