Restriction map for
pF1KB7006
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# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AhdI Aor51HI ApaI AscI
AspEI AspI AviII BbrPI BcgI
BciVI BfrI BsaAI BsaBI BseAI
BsiWI BsmBI BspEI BspHI BspLU11I
BsrBI BssHII Bst1107I BstEII BstPI
BstZ17I CpoI Eam1105I Eco47III EcoO65I
EcoT22I FseI FspI HgaI HincII
HindII HpaI KspI MamI MfeI
MluI MroI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PflFI PmaCI
PmeI PmlI PshAI Psp1406I RcaI
RsrII SacII SalI SbfI ScaI
SfiI SmaI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcyI 1842 GGCGCC G[GA]^CG[CT]C
AgeI 1038 ACCGGT A^CCGGT
Alw44I 2913 GTGCAC G^TGCAC
ApaLI 2913 GTGCAC G^TGCAC
AseI 721 ATTAAT AT^TAAT
AsnI 721 ATTAAT AT^TAAT
AvrII 2617 CCTAGG C^CTAGG
BanII 541 GAGCTC G[GA]GC[CT]^C
BbeI 1842 GGCGCC GGCGC^C
BbvCI 385 GCTGAGG CCTCAGC(-5/-2)
BclI 996 TGATCA T^GATCA
BlnI 2617 CCTAGG C^CTAGG
BlpI 1057 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1057 GCTCAGC GC^T[ACGT]AGC
BsaHI 1842 GGCGCC G[GA]^CG[CT]C
BsmFI 2608 GTCCC GGGAC(10/14)
Bsp1407I 1470 TGTACA T^GTACA
BsrGI 1470 TGTACA T^GTACA
BstAPI 1410 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3143 TTCGAA TT^CGAA
CelII 1057 GCTCAGC GC^T[ACGT]AGC
DraI 678 TTTAAA TTT^AAA
DrdI 1531 GACCGTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2940 CCTCTTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2316 GAATTC G^AATTC
EcoRV 2462 GATATC GAT^ATC
FbaI 996 TGATCA T^GATCA
HaeII 1842 GGCGCC [GA]GCGC^[CT]
Hin1I 1842 GGCGCC G[GA]^CG[CT]C
KasI 1842 GGCGCC G^GCGCC
MaeII 1296 ACGT A^CGT
NarI 1842 GGCGCC GG^CGCC
NspV 3143 TTCGAA TT^CGAA
PinAI 1038 ACCGGT A^CCGGT
PpuMI 2606 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 721 ATTAAT AT^TAAT
PvuI 23 CGATCG CGAT^CG
SacI 541 GAGCTC GAGCT^C
SapI 1213 GAAGAGC GCTCTTC(1/4)
SexAI 65 ACCTGGT A^CC[AT]GGT
SfoI 1842 GGCGCC GGC^GCC
SfuI 3143 TTCGAA TT^CGAA
SgrAI 1037 CACCGGTG C[GA]^CCGG[CT]G
SspBI 1470 TGTACA T^GTACA
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# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 2824 GGTACC G^GTACC
3191 GGTACC
AccII 17 CGCG CG^CG
123 CGCG
AocI 1699 CCTCAGG CC^T[ACGT]AGG
2509 CCTCAGG
Asp700I 1975 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2417 GAAACATTTC
Asp718I 2824 GGTACC G^GTACC
3191 GGTACC
BbsI 1346 GAAGAC GAAGAC(2/6)
1995 GAAGAC
BpmI 1367 CTCCAG CTGGAG(16/14)
37 CTGGAG
BpuAI 1346 GAAGAC GAAGAC(2/6)
1995 GAAGAC
BsaI 2086 GAGACC GGTCTC(1/5)
2025 GGTCTC
BsgI 139 GTGCAG GTGCAG(16/14)
938 GTGCAG
BsmI 246 GAATGC GAATGC(1/-1)
1979 GCATTC
BspDI 1162 ATCGAT AT^CGAT
2107 ATCGAT
BssSI 79 CACGAG CACGAG(-5/-1)
1706 CACGAG
BstUI 17 CGCG CG^CG
123 CGCG
Bsu36I 1699 CCTCAGG CC^T[ACGT]AGG
2509 CCTCAGG
ClaI 1162 ATCGAT AT^CGAT
2107 ATCGAT
DraII 1602 GGGGCCT [GA]G^G[ACGT]CC[CT]
2606 AGGTCCC
DraIII 64 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
559 CACCGAGTG
EagI 146 CGGCCG C^GGCCG
1928 CGGCCG
EclXI 146 CGGCCG C^GGCCG
1928 CGGCCG
Eco52I 146 CGGCCG C^GGCCG
1928 CGGCCG
Eco81I 1699 CCTCAGG CC^T[ACGT]AGG
2509 CCTCAGG
EcoO109I 1602 GGGGCCT [GA]G^G[ACGT]CC[CT]
2606 AGGTCCC
KpnI 2824 GGTACC GGTAC^C
3191 GGTACC
MslI 1499 CACAGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3057 CACAGAGATG
MvnI 17 CGCG CG^CG
123 CGCG
PaeR7I 311 CTCGAG C^TCGAG
1376 CTCGAG
PflMI 839 CCAAAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1192 CCAAATGGTGG
PstI 1408 CTGCAG CTGCA^G
1417 CTGCAG
Van91I 839 CCAAAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1192 CCAAATGGTGG
XhoI 311 CTCGAG C^TCGAG
1376 CTCGAG
XmnI 1975 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2417 GAAACATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BalI BamHI BanI BbvI BcnI
BfaI BglI BglII BmyI BsaJI
BsaWI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DsaI
EaeI EarI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII