Restriction map for
pF1KB9022
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# NO CUT ENZYMES ( 81 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AocI Aor51HI
AscI AseI AsnI Asp718I AviII
AvrII BbrPI BbvCI BcgI BclI
BfrI BglII BlnI BsaAI BsaWI
BseAI BsiWI BspDI BspEI BspHI
BspLU11I BssSI Bst1107I BstBI BstZ17I
Bsu36I ClaI CpoI Eco47III Eco81I
EcoT22I FbaI FspI HincII HindII
HindIII HpaI KpnI MfeI MluI
MroI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
XhoI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AhdI 4206 GACAGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4403 GTGCAC G^TGCAC
ApaLI 4403 GTGCAC G^TGCAC
Asp700I 3351 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4206 GACAGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 176 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 3852 GGCGCC GGCGC^C
BlpI 2335 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2335 GCTCAGC GC^T[ACGT]AGC
BsgI 2231 GTGCAG GTGCAG(16/14)
BsiEI 594 CGGCCG CG[GA][CT]^CG
Bsp1407I 1453 TGTACA T^GTACA
BsrDI 4973 GCAATG GCAATG(2/0)
BsrGI 1453 TGTACA T^GTACA
BssHII 15 GCGCGC G^CGCGC
BstAPI 1072 GCAAAAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 2335 GCTCAGC GC^T[ACGT]AGC
DraIII 3836 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 594 CGGCCG C^GGCCG
Eam1105I 4206 GACAGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 594 CGGCCG C^GGCCG
Eco52I 594 CGGCCG C^GGCCG
EcoNI 163 CCTGAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 353 GAATTC G^AATTC
EcoRV 542 GATATC GAT^ATC
KasI 3852 GGCGCC G^GCGCC
MslI 1986 CACAGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 3852 GGCGCC GG^CGCC
PflFI 176 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 247 CTGCAG CTGCA^G
SfcI 247 CTGCAG C^T[GA][CT]AG
SfoI 3852 GGCGCC GGC^GCC
SphI 2795 GCATGC GCATG^C
SspBI 1453 TGTACA T^GTACA
Tth111I 176 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4938 TCTAGA T^CTAGA
XmnI 3351 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AcsI 353 GAATTC [GA]^AATT[CT]
3291 AAATTT
ApoI 353 GAATTC [GA]^AATT[CT]
3291 AAATTT
BbsI 3302 GAAGAC GAAGAC(2/6)
1481 GTCTTC
BfaI 1069 CTAG C^TAG
4939 CTAG
BpuAI 3302 GAAGAC GAAGAC(2/6)
1481 GTCTTC
BsaBI 973 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1183 GATGGAGATC
BsrBI 1672 GAGCGG CCGCTC(-3/-3)
3322 GAGCGG
BstXI 1417 CCAAAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4837 CCAGCTGGGTGG
DraI 2716 TTTAAA TTT^AAA
2896 TTTAAA
DrdI 614 GACAGATGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3112 GACATCGGAGTC
FseI 24 GGCCGGCC GGCCGG^CC
5082 GGCCGGCC
HaeII 2623 GGCGCT [GA]GCGC^[CT]
3852 GGCGCC
MaeI 1069 CTAG C^TAG
4939 CTAG
MamI 973 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1183 GATGGAGATC
NspI 606 GCATGT [GA]CATG^[CT]
2795 GCATGC
PvuII 4838 CAGCTG CAG^CTG
4932 CAGCTG
SapI 1370 GAAGAGC GCTCTTC(1/4)
3913 GCTCTTC
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# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbvI BciVI BcnI BglI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstEII BstF5I
BstNI BstPI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I KspI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV PflMI PleI
PpuMI PshAI RsaI SacI SacII
Sau3AI Sau96I ScrFI SexAI SfaNI
SfiI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI