Restriction map for
pF1KB9023
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# NO CUT ENZYMES ( 59 enzymes) #
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AatII AccIII AclI AcyI AgeI
AocI AscI AseI AsnI AvrII
BbeI BlnI BsaHI BseAI BsiWI
BspDI BspEI BstEII BstPI Bsu36I
ClaI CpoI Eco81I EcoNI EcoO65I
EcoRV EcoT22I FseI HgaI Hin1I
HindIII HpaI KasI MluI MroI
NarI NdeI NheI NotI NruI
NsiI PacI PaeR7I PinAI PmeI
PshBI Psp1406I RsrII SalI ScaI
SfiI SfoI SgrAI SnaBI SpeI
SspI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 65 enzymes) #
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AflII 1371 CTTAAG C^TTAAG
AflIII 2303 ACATGT A^C[GA][CT]GT
AhdI 3920 GACGGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3059 AGCGCT AGC^GCT
AspEI 3920 GACGGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2362 TGCGCA TGC^GCA
BbrPI 1621 CACGTG CAC^GTG
BbvCI 1897 GCTGAGG CCTCAGC(-5/-2)
BcgI 185 CGAATCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1050 GGATAC GTATCC(6/5)
BfaI 734 CTAG C^TAG
BfrI 1371 CTTAAG C^TTAAG
BglII 3678 AGATCT A^GATCT
BlpI 164 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 164 GCTCAGC GC^T[ACGT]AGC
BsaBI 4048 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2274 TCCGGT [AT]^CCGG[AT]
BsmI 2683 GAATGC GAATGC(1/-1)
Bsp1407I 3898 TGTACA T^GTACA
BspHI 2964 TCATGA T^CATGA
BspLU11I 2303 ACATGT A^CATGT
BsrBI 3222 CCGCTC CCGCTC(-3/-3)
BsrDI 1679 GCAATG GCAATG(2/0)
BsrGI 3898 TGTACA T^GTACA
BssSI 342 CACGAG CACGAG(-5/-1)
Bst1107I 3251 GTATAC GTA^TAC
BstBI 1337 TTCGAA TT^CGAA
BstZ17I 3251 GTATAC GTA^TAC
CelII 164 GCTCAGC GC^T[ACGT]AGC
DraI 1134 TTTAAA TTT^AAA
DrdI 3402 GACCCTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 25 CGGCCG C^GGCCG
Eam1105I 3920 GACGGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 25 CGGCCG C^GGCCG
Eco47III 3059 AGCGCT AGC^GCT
Eco52I 25 CGGCCG C^GGCCG
FspI 2362 TGCGCA TGC^GCA
HincII 2753 GTCAAC GT[CT]^[GA]AC
HindII 2753 GTCAAC GT[CT]^[GA]AC
KspI 83 CCGCGG CCGC^GG
MaeI 734 CTAG C^TAG
MamI 4048 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1754 CAATTG C^AATTG
MunI 1754 CAATTG C^AATTG
NaeI 2650 GCCGGC GCC^GGC
NgoMIV 2650 GCCGGC G^CCGGC
NspV 1337 TTCGAA TT^CGAA
PflMI 1839 CCATGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1621 CACGTG CAC^GTG
PmlI 1621 CACGTG CAC^GTG
PpuMI 3662 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 2141 GACACCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 3536 CGATCG CGAT^CG
RcaI 2964 TCATGA T^CATGA
SacI 103 GAGCTC GAGCT^C
SacII 83 CCGCGG CCGC^GG
SapI 105 GCTCTTC GCTCTTC(1/4)
SbfI 141 CCTGCAGG CCTGCA^GG
SexAI 2117 ACCAGGT A^CC[AT]GGT
SfuI 1337 TTCGAA TT^CGAA
Sse8387I 141 CCTGCAGG CCTGCA^GG
SspBI 3898 TGTACA T^GTACA
StuI 1761 AGGCCT AGG^CCT
Van91I 1839 CCATGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2982 CCAGACTCCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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Acc65I 2372 GGTACC G^GTACC
2977 GGTACC
Alw44I 1639 GTGCAC G^TGCAC
2667 GTGCAC
ApaI 1125 GGGCCC GGGCC^C
2864 GGGCCC
ApaLI 1639 GTGCAC G^TGCAC
2667 GTGCAC
Asp718I 2372 GGTACC G^GTACC
2977 GGTACC
BbsI 1402 GAAGAC GAAGAC(2/6)
1429 GTCTTC
BclI 2531 TGATCA T^GATCA
3803 TGATCA
BpuAI 1402 GAAGAC GAAGAC(2/6)
1429 GTCTTC
BsaI 854 GAGACC GGTCTC(1/5)
1168 GAGACC
BsiEI 25 CGGCCG CG[GA][CT]^CG
3536 CGATCG
BsmBI 1873 GAGACG CGTCTC(1/5)
3532 GAGACG
BssHII 420 GCGCGC G^CGCGC
442 GCGCGC
BstAPI 1563 GCACTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1822 GCAGGTGCTGC
DraIII 1588 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
2813 CACCCTGTG
EcoRI 3543 GAATTC G^AATTC
3797 GAATTC
FbaI 2531 TGATCA T^GATCA
3803 TGATCA
KpnI 2372 GGTACC GGTAC^C
2977 GGTACC
NcoI 2607 CCATGG C^CATGG
3272 CCATGG
SmaI 1863 CCCGGG CCC^GGG
3101 CCCGGG
SphI 1491 GCATGC GCATG^C
1662 GCATGC
XmaCI 1863 CCCGGG C^CCGGG
3101 CCCGGG
XmaI 1863 CCCGGG C^CCGGG
3101 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AspI AvaI AvaII BalI
BamHI BanI BanII BbvI BcnI
BglI BmyI BpmI BsaAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflFI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XhoII XmnI