Restriction map for
pF1KB9213
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccI AccIII AflII
AflIII AgeI AhdI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AviII AvrII BamHI BbeI
BbrPI BcgI BclI BfrI BglI
BlnI BsaBI BsaWI BseAI BsiEI
BsiWI BsoBI BspDI BspEI BspHI
BspLU11I BssHII Bst1107I BstBI BstEII
BstPI BstXI BstZ17I CfoI ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HhaI HinP1I HpaI KasI KpnI
KspI MamI MluI MroI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
PvuI RcaI RsrII SacII SalI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspI
StuI SwaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccII 2106 CGCG CG^CG
AclI 2121 AACGTT AA^CGTT
AcsI 388 AAATTC [GA]^AATT[CT]
AcyI 135 GACGCC G[GA]^CG[CT]C
Alw44I 1565 GTGCAC G^TGCAC
AlwI 1225 GATCC GGATC(4/5)
AocI 1519 CCTCAGG CC^T[ACGT]AGG
ApaI 1137 GGGCCC GGGCC^C
ApaLI 1565 GTGCAC G^TGCAC
ApoI 388 AAATTC [GA]^AATT[CT]
AspI 109 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1169 TGGCCA TGG^CCA
BbvCI 829 GCTGAGG CCTCAGC(-5/-2)
BciVI 759 GGATAC GTATCC(6/5)
BlpI 1591 GCTAAGC GC^T[ACGT]AGC
BpmI 1041 CTGGAG CTGGAG(16/14)
Bpu1102I 1591 GCTAAGC GC^T[ACGT]AGC
BsaAI 1268 TACGTG [CT]AC^GT[GA]
BsaHI 135 GACGCC G[GA]^CG[CT]C
BseRI 1049 CTCCTC GAGGAG(10/8)
BsmBI 1663 GAGACG CGTCTC(1/5)
BsmI 1717 GCATTC GAATGC(1/-1)
Bsp1407I 741 TGTACA T^GTACA
BspMI 29 ACCTGC ACCTGC(4/8)
BsrBI 308 GAGCGG CCGCTC(-3/-3)
BsrFI 359 ACCGGC [GA]^CCGG[CT]
BsrGI 741 TGTACA T^GTACA
BstUI 2106 CGCG CG^CG
Bsu36I 1519 CCTCAGG CC^T[ACGT]AGG
CelII 1591 GCTAAGC GC^T[ACGT]AGC
Cfr10I 359 ACCGGC [GA]^CCGG[CT]
DraIII 1265 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2431 GACAGCGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1519 CCTCAGG CC^T[ACGT]AGG
EcoNI 30 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 135 GACGCC G[GA]^CG[CT]C
HindIII 1855 AAGCTT A^AGCTT
MfeI 1756 CAATTG C^AATTG
MluNI 1169 TGGCCA TGG^CCA
MscI 1169 TGGCCA TGG^CCA
MunI 1756 CAATTG C^AATTG
MvnI 2106 CGCG CG^CG
NdeI 374 CATATG CA^TATG
NspI 1688 ACATGC [GA]CATG^[CT]
PflFI 109 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 973 CCAGAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 2121 AACGTT AA^CGTT
SacI 484 GAGCTC GAGCT^C
SapI 2413 GCTCTTC GCTCTTC(1/4)
SbfI 30 CCTGCAGG CCTGCA^GG
SexAI 945 ACCTGGT A^CC[AT]GGT
Sse8387I 30 CCTGCAGG CCTGCA^GG
SspBI 741 TGTACA T^GTACA
Tth111I 109 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 973 CCAGAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BanI 327 GGTGCC G^G[CT][GA]CC
982 GGCACC
BbsI 817 GAAGAC GAAGAC(2/6)
1637 GAAGAC
BcnI 200 CCCGG CC^[GC]GG
839 CCGGG
BpuAI 817 GAAGAC GAAGAC(2/6)
1637 GAAGAC
BsaI 508 GAGACC GGTCTC(1/5)
1297 GAGACC
BsrDI 2019 CATTGC GCAATG(2/0)
554 GCAATG
BssSI 1729 CACGAG CACGAG(-5/-1)
1562 CTCGTG
BstAPI 1264 GCACTACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2259 GCATCTCATGC
DraI 661 TTTAAA TTT^AAA
1797 TTTAAA
DsaI 1166 CCATGG C^C[GA][CT]GG
2240 CCATGG
EaeI 281 TGGCCG [CT]^GGCC[GA]
1169 TGGCCA
EcoT14I 1166 CCATGG C^C[AT][AT]GG
2240 CCATGG
HincII 132 GTTGAC GT[CT]^[GA]AC
1576 GTTGAC
HindII 132 GTTGAC GT[CT]^[GA]AC
1576 GTTGAC
NciI 200 CCCGG CC^[GC]GG
839 CCGGG
NcoI 1166 CCATGG C^CATGG
2240 CCATGG
PpuMI 26 AGGACCT [GA]G^G[AT]CC[CT]
612 GGGACCT
PstI 31 CTGCAG CTGCA^G
1766 CTGCAG
SmlI 219 CTTGAG C^T[CT][GA]AG
1455 CTTGAG
StyI 1166 CCATGG C^C[AT][AT]GG
2240 CCATGG
Tsp45I 1912 GTGAC ^GT[GC]AC
2429 GTGAC
XcmI 791 CCAGTGTCATACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1175 CCAGAAAGGAACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaII BanII BbvI BfaI BglII
BmyI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HgaI HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XbaI XhoII