Restriction map for
pF1KB9288
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# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccI AccIII AflII
AgeI Alw44I ApaLI AscI AseI
AsnI Asp718I AspI AviII AvrII
BbeI BciVI BfrI BglII BlnI
BseAI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BspHI BsrBI BsrDI
BssHII BssSI Bst1107I BstZ17I ClaI
CpoI DrdI EagI EclXI Eco52I
EcoNI EcoRV EcoT22I FseI FspI
KasI KpnI KspI MfeI MluI
MroI MunI NarI NheI NotI
NruI NsiI PacI PflFI PflMI
PinAI PmeI PshBI PvuI PvuII
RcaI RsrII SacII SalI SbfI
ScaI SfiI SfoI SgrAI SnaBI
SpeI Sse8387I SspI Tth111I Van91I
XbaI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccII 1586 CGCG CG^CG
AclI 2513 AACGTT AA^CGTT
AcyI 1652 GACGCC G[GA]^CG[CT]C
AhdI 1476 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2597 AGCGCT AGC^GCT
AspEI 1476 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 147 TGGCCA TGG^CCA
BamHI 2663 GGATCC G^GATCC
BanI 641 GGTGCC G^G[CT][GA]CC
BbrPI 572 CACGTG CAC^GTG
BbvCI 249 GCTGAGG CCTCAGC(-5/-2)
BcgI 597 CGATACTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1752 TGATCA T^GATCA
BglI 2294 GCCAGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 895 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 895 GCTCAGC GC^T[ACGT]AGC
BsaBI 1904 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1652 GACGCC G[GA]^CG[CT]C
BsaWI 1565 TCCGGT [AT]^CCGG[AT]
Bsp1407I 773 TGTACA T^GTACA
BspLU11I 1197 ACATGT A^CATGT
BsrFI 221 GCCGGC [GA]^CCGG[CT]
BsrGI 773 TGTACA T^GTACA
BstAPI 1796 GCAGCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3054 TTCGAA TT^CGAA
BstEII 720 GGTGACC G^GT[ACGT]ACC
BstPI 720 GGTGACC G^GT[ACGT]ACC
BstUI 1586 CGCG CG^CG
BstXI 7 CCAGGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 895 GCTCAGC GC^T[ACGT]AGC
Cfr10I 221 GCCGGC [GA]^CCGG[CT]
DraI 1537 TTTAAA TTT^AAA
DraIII 1435 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 871 CCATGG C^C[GA][CT]GG
EaeI 147 TGGCCA [CT]^GGCC[GA]
Eam1105I 1476 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2597 AGCGCT AGC^GCT
EcoO65I 720 GGTGACC G^GT[ACGT]ACC
FbaI 1752 TGATCA T^GATCA
HaeII 2597 AGCGCT [GA]GCGC^[CT]
HgaI 1652 GACGC GACGC(5/10)
Hin1I 1652 GACGCC G[GA]^CG[CT]C
HpaI 2650 GTTAAC GTT^AAC
MamI 1904 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 147 TGGCCA TGG^CCA
MscI 147 TGGCCA TGG^CCA
MspA1I 2024 CCGCTG C[AC]G^C[GT]G
MvnI 1586 CGCG CG^CG
NaeI 221 GCCGGC GCC^GGC
NcoI 871 CCATGG C^CATGG
NgoMIV 221 GCCGGC G^CCGGC
NspV 3054 TTCGAA TT^CGAA
PaeR7I 3002 CTCGAG C^TCGAG
PmaCI 572 CACGTG CAC^GTG
PmlI 572 CACGTG CAC^GTG
Psp1406I 2513 AACGTT AA^CGTT
SfuI 3054 TTCGAA TT^CGAA
SspBI 773 TGTACA T^GTACA
SwaI 1536 ATTTAAAT ATTT^AAAT
XcmI 451 CCAGATGTGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3002 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AocI 901 CCTAAGG CC^T[ACGT]AGG
2209 CCTGAGG
ApaI 202 GGGCCC GGGCC^C
435 GGGCCC
BsaAI 572 CACGTG [CT]AC^GT[GA]
2549 CACGTA
BspMI 837 ACCTGC ACCTGC(4/8)
11 GCAGGT
BstYI 278 AGATCC [GA]^GATC[CT]
2663 GGATCC
Bsu36I 901 CCTAAGG CC^T[ACGT]AGG
2209 CCTGAGG
CfoI 1585 GCGC GCG^C
2598 GCGC
Eco81I 901 CCTAAGG CC^T[ACGT]AGG
2209 CCTGAGG
EcoRI 1346 GAATTC G^AATTC
2102 GAATTC
EcoT14I 269 CCAAGG C^C[AT][AT]GG
871 CCATGG
HhaI 1585 GCGC GCG^C
2598 GCGC
HinP1I 1585 GCGC G^CGC
2598 GCGC
HincII 2089 GTTGAC GT[CT]^[GA]AC
2650 GTTAAC
HindII 2089 GTTGAC GT[CT]^[GA]AC
2650 GTTAAC
HindIII 1643 AAGCTT A^AGCTT
2870 AAGCTT
MflI 278 AGATCC [GA]^GATC[CT]
2663 GGATCC
PshAI 1652 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1999 GACTGCAGTC
SacI 328 GAGCTC GAGCT^C
2883 GAGCTC
SapI 1071 GAAGAGC GCTCTTC(1/4)
168 GCTCTTC
SexAI 653 ACCAGGT A^CC[AT]GGT
667 ACCAGGT
SmaI 432 CCCGGG CCC^GGG
645 CCCGGG
SmlI 1523 CTCAAG C^T[CT][GA]AG
3002 CTCGAG
SphI 18 GCATGC GCATG^C
2190 GCATGC
StuI 253 AGGCCT AGG^CCT
2306 AGGCCT
StyI 269 CCAAGG C^C[AT][AT]GG
871 CCATGG
XhoII 278 AGATCC [GA]^GATC[CT]
2663 GGATCC
XmaCI 432 CCCGGG C^CCGGG
645 CCCGGG
XmaI 432 CCCGGG C^CCGGG
645 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspI MvaI MwoI NciI
NdeI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmnI