Restriction map for
pF1KB9425
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
Acc65I AccI AccIII AclI AflIII
AgeI AhdI Alw44I AlwNI AocI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BfaI
BglI BglII BlnI BsaAI BsaWI
BseAI BsiWI BsmI Bsp1407I BspEI
BspLU11I BsrBI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CpoI DraI Eam1105I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FseI FspI
HpaI KasI KpnI KspI MaeI
MluI MroI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SfiI SfoI SfuI SgrAI
SnaBI SpeI SspBI SspI StuI
SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 146 CGCG CG^CG
AflII 22 CTTAAG C^TTAAG
Aor51HI 1496 AGCGCT AGC^GCT
ApaI 1772 GGGCCC GGGCC^C
AvaI 2108 CCCGGG C^[CT]CG[GA]G
BclI 380 TGATCA T^GATCA
BfrI 22 CTTAAG C^TTAAG
BlpI 1887 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1887 GCTCAGC GC^T[ACGT]AGC
BsaBI 27 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 2108 CCCGGG C^[CT]CG[GA]G
BspHI 1922 TCATGA T^CATGA
BspMI 613 ACCTGC ACCTGC(4/8)
BsrFI 2118 GCCGGT [GA]^CCGG[CT]
BssSI 637 CACGAG CACGAG(-5/-1)
BstAPI 804 GCAGGGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 146 CGCG CG^CG
BstYI 1730 AGATCC [GA]^GATC[CT]
CelII 1887 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2118 GCCGGT [GA]^CCGG[CT]
DraIII 651 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 199 GACGAGTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1760 CCGTGG C^C[GA][CT]GG
Eco47III 1496 AGCGCT AGC^GCT
EcoRI 2170 GAATTC G^AATTC
EcoT14I 1433 CCAAGG C^C[AT][AT]GG
FauI 987 CCCGC CCCGC(4/6)
FbaI 380 TGATCA T^GATCA
HindIII 1916 AAGCTT A^AGCTT
MamI 27 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2144 CAATTG C^AATTG
MflI 1730 AGATCC [GA]^GATC[CT]
MunI 2144 CAATTG C^AATTG
MvnI 146 CGCG CG^CG
PvuII 1153 CAGCTG CAG^CTG
RcaI 1922 TCATGA T^CATGA
SacI 640 GAGCTC GAGCT^C
SbfI 1734 CCTGCAGG CCTGCA^GG
ScaI 903 AGTACT AGT^ACT
SexAI 689 ACCAGGT A^CC[AT]GGT
SmaI 2108 CCCGGG CCC^GGG
SphI 449 GCATGC GCATG^C
Sse8387I 1734 CCTGCAGG CCTGCA^GG
StyI 1433 CCAAGG C^C[AT][AT]GG
XcmI 869 CCAACTGTGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1730 AGATCC [GA]^GATC[CT]
XmaCI 2108 CCCGGG C^CCGGG
XmaI 2108 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AatII 1324 GACGTC GACGT^C
2080 GACGTC
AcsI 1507 AAATTC [GA]^AATT[CT]
2170 GAATTC
AlwI 1028 GATCC GGATC(4/5)
1731 GATCC
ApoI 1507 AAATTC [GA]^AATT[CT]
2170 GAATTC
Asp700I 1666 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1914 GAAAGCTTTC
BanI 632 GGTGCC G^G[CT][GA]CC
976 GGTGCC
BbsI 174 GAAGAC GAAGAC(2/6)
2197 GAAGAC
BciVI 592 GGATAC GTATCC(6/5)
737 GTATCC
BpmI 355 CTGGAG CTGGAG(16/14)
2026 CTGGAG
BpuAI 174 GAAGAC GAAGAC(2/6)
2197 GAAGAC
BsiEI 94 CGGCCG CG[GA][CT]^CG
454 CGGCCG
BsmBI 1139 CGTCTC CGTCTC(1/5)
2078 GAGACG
BspDI 385 ATCGAT AT^CGAT
1025 ATCGAT
ClaI 385 ATCGAT AT^CGAT
1025 ATCGAT
EagI 94 CGGCCG C^GGCCG
454 CGGCCG
EclXI 94 CGGCCG C^GGCCG
454 CGGCCG
Eco52I 94 CGGCCG C^GGCCG
454 CGGCCG
HincII 499 GTCAAC GT[CT]^[GA]AC
715 GTCAAC
HindII 499 GTCAAC GT[CT]^[GA]AC
715 GTCAAC
NspI 449 GCATGC [GA]CATG^[CT]
719 ACATGC
PflMI 59 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
78 CCATGCGGTGG
SapI 427 GAAGAGC GCTCTTC(1/4)
2113 GAAGAGC
Van91I 59 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
78 CCATGCGGTGG
XmnI 1666 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1914 GAAAGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcyI AfaI AluI AspHI
AvaII BalI BanII BbvI BcnI
BmyI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I