Restriction map for
pF1KB9426
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# NO CUT ENZYMES ( 113 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI AocI
Aor51HI ApoI AscI AseI AsnI
Asp718I AspEI AvrII BbrPI BbvCI
BciVI BclI BfaI BfrI BglI
BlnI BlpI Bpu1102I BseAI BsiWI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
HincII HindII HindIII HpaI KpnI
KspI MaeI MluI MroI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I RsrII SacII
SalI SbfI ScaI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI StyI SwaI
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 33 enzymes) #
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Alw44I 615 GTGCAC G^TGCAC
ApaI 1856 GGGCCC GGGCC^C
ApaLI 615 GTGCAC G^TGCAC
AspI 1447 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 326 TGCGCA TGC^GCA
BbeI 337 GGCGCC GGCGC^C
BcgI 617 GCACCTGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1434 AGATCT A^GATCT
BsaBI 723 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 443 ACCGGA [AT]^CCGG[AT]
BseRI 208 GAGGAG GAGGAG(10/8)
BsgI 300 CTGCAC GTGCAG(16/14)
BsmBI 265 GAGACG CGTCTC(1/5)
BsmI 1192 GAATGC GAATGC(1/-1)
BspHI 1763 TCATGA T^CATGA
BsrFI 334 ACCGGC [GA]^CCGG[CT]
Cfr10I 334 ACCGGC [GA]^CCGG[CT]
DraIII 286 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1529 CCACGG C^C[GA][CT]GG
FspI 326 TGCGCA TGC^GCA
HgaI 267 GACGC GACGC(5/10)
KasI 337 GGCGCC G^GCGCC
MamI 723 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1970 CAATTG C^AATTG
MunI 1970 CAATTG C^AATTG
NarI 337 GGCGCC GG^CGCC
PflFI 1447 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 1278 CGATCG CGAT^CG
RcaI 1763 TCATGA T^CATGA
SacI 1364 GAGCTC GAGCT^C
SexAI 149 ACCAGGT A^CC[AT]GGT
SfoI 337 GGCGCC GGC^GCC
Tth111I 1447 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 37 enzymes) #
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AccI 1603 GTAGAC GT^[AC][GT]AC
1714 GTCTAC
AccII 157 CGCG CG^CG
1653 CGCG
AcyI 267 GACGCC G[GA]^CG[CT]C
337 GGCGCC
AlwNI 115 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
316 CAGGAGCTG
Asp700I 941 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2015 GAAACTTTTC
BalI 293 TGGCCA TGG^CCA
524 TGGCCA
BamHI 1214 GGATCC G^GATCC
1748 GGATCC
BanI 10 GGCACC G^G[CT][GA]CC
337 GGCGCC
BanII 1364 GAGCTC G[GA]GC[CT]^C
1856 GGGCCC
BbsI 183 GAAGAC GAAGAC(2/6)
256 GTCTTC
BcnI 410 CCGGG CC^[GC]GG
937 CCCGG
BpuAI 183 GAAGAC GAAGAC(2/6)
256 GTCTTC
BsaAI 198 CACGTA [CT]AC^GT[GA]
528 CACGTA
BsaHI 267 GACGCC G[GA]^CG[CT]C
337 GGCGCC
BsiEI 332 CGACCG CG[GA][CT]^CG
1278 CGATCG
BsmFI 1980 GGGAC GGGAC(10/14)
81 GTCCC
BsrDI 1517 GCAATG GCAATG(2/0)
1615 GCAATG
BstUI 157 CGCG CG^CG
1653 CGCG
EarI 97 CTCTTC CTCTTC(1/4)
1951 GAAGAG
EcoNI 1683 CCTCTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1797 CCTGACTCAGG
FauI 74 CCCGC CCCGC(4/6)
1229 CCCGC
HaeII 337 GGCGCC [GA]GCGC^[CT]
470 AGCGCC
Hin1I 267 GACGCC G[GA]^CG[CT]C
337 GGCGCC
Ksp632I 97 CTCTTC CTCTTC(1/4)
1951 GAAGAG
MluNI 293 TGGCCA TGG^CCA
524 TGGCCA
MscI 293 TGGCCA TGG^CCA
524 TGGCCA
MslI 1071 CATCCGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1732 CATGAAGATG
MvnI 157 CGCG CG^CG
1653 CGCG
NciI 410 CCGGG CC^[GC]GG
937 CCCGG
PaeR7I 244 CTCGAG C^TCGAG
370 CTCGAG
PleI 1800 GACTC GAGTC(4/5)
494 GAGTC
PvuII 115 CAGCTG CAG^CTG
1861 CAGCTG
SapI 1951 GAAGAGC GCTCTTC(1/4)
96 GCTCTTC
SspI 679 AATATT AAT^ATT
943 AATATT
XcmI 498 CCACCAGATCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1778 CCACCATGCGCTTGG
XhoI 244 CTCGAG C^TCGAG
370 CTCGAG
XmnI 941 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2015 GAAACTTTTC
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# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BbvI BmyI BpmI
BsaI BsaJI BsiHKAI BsiYI BslI
BsmAI BsoBI Bsp1286I BsrI BssKI
BssSI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PflMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII