Restriction map for
pF1KB9429
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AviII AvrII BanII BbeI BbrPI
BciVI BfrI BglI BglII BlnI
BsaAI BsaBI BsaHI BsaI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstEII
BstPI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT22I FseI FspI Hin1I
HindIII HpaI KasI KpnI KspI
MaeII MamI MfeI MluI MroI
MslI MspA1I MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 1081 GTCTAC GT^[AC][GT]AC
AspI 332 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 82 CCCGAG C^[CT]CG[GA]G
BamHI 1437 GGATCC G^GATCC
BbvCI 373 GCTGAGG CCTCAGC(-5/-2)
BcgI 360 CGACCTGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 393 TGATCA T^GATCA
BcnI 439 CCGGG CC^[GC]GG
BlpI 1279 GCTCAGC GC^T[ACGT]AGC
BmyI 818 GTGCCC G[AGT]GC[ACT]^C
Bpu1102I 1279 GCTCAGC GC^T[ACGT]AGC
BsaWI 1311 ACCGGA [AT]^CCGG[AT]
BsoBI 82 CCCGAG C^[CT]CG[GA]G
Bsp1286I 818 GTGCCC G[AGT]GC[ACT]^C
BsrBI 1632 CCGCTC CCGCTC(-3/-3)
BstAPI 261 GCAGACTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1220 TTCGAA TT^CGAA
CelII 1279 GCTCAGC GC^T[ACGT]AGC
DraII 1007 GGGTCCC [GA]G^G[ACGT]CC[CT]
DrdI 1072 GACTTTACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1007 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRV 911 GATATC GAT^ATC
FbaI 393 TGATCA T^GATCA
HgaI 553 GACGC GACGC(5/10)
HincII 744 GTTGAC GT[CT]^[GA]AC
HindII 744 GTTGAC GT[CT]^[GA]AC
MseI 845 TTAA T^TAA
NciI 439 CCGGG CC^[GC]GG
NcoI 1457 CCATGG C^CATGG
NspV 1220 TTCGAA TT^CGAA
PflFI 332 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 705 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1007 GGGTCCC [GA]G^G[AT]CC[CT]
SfcI 1083 CTACAG C^T[GA][CT]AG
SfuI 1220 TTCGAA TT^CGAA
SmlI 1325 CTCAAG C^T[CT][GA]AG
TfiI 322 GATTC G^A[AT]TC
Tru9I 845 TTAA T^TAA
Tth111I 332 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 705 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 229 TCTAGA T^CTAGA
XcmI 1442 CCAACGATCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccII 50 CGCG CG^CG
555 CGCG
AcsI 475 AAATTT [GA]^AATT[CT]
808 GAATTT
AfaI 897 GTAC GT^AC
1629 GTAC
AlwNI 13 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
262 CAGACTCTG
Aor51HI 1061 AGCGCT AGC^GCT
1238 AGCGCT
ApoI 475 AAATTT [GA]^AATT[CT]
808 GAATTT
BalI 1454 TGGCCA TGG^CCA
1644 TGGCCA
BanI 1156 GGTGCC G^G[CT][GA]CC
1581 GGCACC
BstUI 50 CGCG CG^CG
555 CGCG
BstYI 1316 AGATCC [GA]^GATC[CT]
1437 GGATCC
DsaI 1457 CCATGG C^C[GA][CT]GG
1671 CCACGG
EaeI 1454 TGGCCA [CT]^GGCC[GA]
1644 TGGCCA
Eco47III 1061 AGCGCT AGC^GCT
1238 AGCGCT
HapII 439 CCGG C^CGG
1312 CCGG
HpaII 439 CCGG C^CGG
1312 CCGG
MflI 1316 AGATCC [GA]^GATC[CT]
1437 GGATCC
MluNI 1454 TGGCCA TGG^CCA
1644 TGGCCA
MscI 1454 TGGCCA TGG^CCA
1644 TGGCCA
MspI 439 CCGG C^CGG
1312 CCGG
MvnI 50 CGCG CG^CG
555 CGCG
RsaI 897 GTAC GT^AC
1629 GTAC
SapI 411 GAAGAGC GCTCTTC(1/4)
671 GAAGAGC
SfaNI 1612 GATGC GCATC(5/9)
178 GCATC
Tsp45I 464 GTGAC ^GT[GC]AC
833 GTCAC
Tsp509I 476 AATT ^AATT
809 AATT
XhoII 1316 AGATCC [GA]^GATC[CT]
1437 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AluI AlwI AvaII BbsI
BbvI BfaI BpmI BpuAI BsaJI
BseRI BsiYI BslI BsmFI BsrI
BssKI BstF5I BstNI BstXI Cac8I
CfoI Cfr13I DdeI DpnII EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HhaI HinP1I HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MvaI MwoI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI StyI TaqI TseI
TspRI TthHB8I