Restriction map for
pF1KB9430
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BalI BamHI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BsrDI
BssSI Bst1107I BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DdeI
DraI DraIII DrdI Eam1105I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI MaeI MaeIII
MamI MfeI MluNI MroI MscI
MslI MunI NdeI NheI NotI
NruI NsiI PacI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SalI SapI SbfI ScaI
SfcI SfiI SgrAI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tsp45I Tsp509I Van91I XbaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 886 GTCTAC GT^[AC][GT]AC
AflIII 1007 ACGCGT A^C[GA][CT]GT
AlwI 315 GATCC GGATC(4/5)
AlwNI 686 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 189 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 54 GAGCTC G[AT]GC[AT]^C
AspI 466 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1167 GGACC G^G[AT]CC
BbsI 1081 GAAGAC GAAGAC(2/6)
BglII 263 AGATCT A^GATCT
BpmI 420 CTCCAG CTGGAG(16/14)
BpuAI 1081 GAAGAC GAAGAC(2/6)
BsiHKAI 54 GAGCTC G[AT]GC[AT]^C
Bsp1407I 776 TGTACA T^GTACA
BspMI 1088 ACCTGC ACCTGC(4/8)
BsrGI 776 TGTACA T^GTACA
BsrI 1259 ACTGG ACTGG(1/-1)
BstAPI 685 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1078 TTCGAA TT^CGAA
BstF5I 654 CATCC GGATG(2/0)
BstXI 269 CCAAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 1166 GGGACCT [GA]G^G[ACGT]CC[CT]
EarI 1038 CTCTTC CTCTTC(1/4)
EcoO109I 1166 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 495 CCATGG C^C[AT][AT]GG
FokI 654 CATCC GGATG(9/13)
Ksp632I 1038 CTCTTC CTCTTC(1/4)
KspI 951 CCGCGG CCGC^GG
MaeII 910 ACGT A^CGT
MluI 1007 ACGCGT A^CGCGT
MseI 1178 TTAA T^TAA
NcoI 495 CCATGG C^CATGG
NspV 1078 TTCGAA TT^CGAA
PflFI 466 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1166 GGGACCT [GA]G^G[AT]CC[CT]
SacI 54 GAGCTC GAGCT^C
SacII 951 CCGCGG CCGC^GG
SexAI 1283 ACCTGGT A^CC[AT]GGT
SfaNI 1274 GCATC GCATC(5/9)
SfuI 1078 TTCGAA TT^CGAA
SmaI 849 CCCGGG CCC^GGG
SspBI 776 TGTACA T^GTACA
StyI 495 CCATGG C^C[AT][AT]GG
TfiI 86 GAATC G^A[AT]TC
Tru9I 1178 TTAA T^TAA
TspRI 480 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 466 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 647 CCAAGCTCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 849 CCCGGG C^CCGGG
XmaI 849 CCCGGG C^CCGGG
XmnI 189 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 381 GTAC GT^AC
777 GTAC
Aor51HI 36 AGCGCT AGC^GCT
837 AGCGCT
BsgI 1224 CTGCAC GTGCAG(16/14)
4 GTGCAG
BsmAI 464 GAGAC GTCTC(1/5)
1256 GAGAC
BssHII 969 GCGCGC G^CGCGC
995 GCGCGC
BstYI 263 AGATCT [GA]^GATC[CT]
314 AGATCC
EagI 431 CGGCCG C^GGCCG
930 CGGCCG
EclXI 431 CGGCCG C^GGCCG
930 CGGCCG
Eco47III 36 AGCGCT AGC^GCT
837 AGCGCT
Eco52I 431 CGGCCG C^GGCCG
930 CGGCCG
HgaI 884 GCGTC GACGC(5/10)
1009 GCGTC
MflI 263 AGATCT [GA]^GATC[CT]
314 AGATCC
MspA1I 686 CAGCGG C[AC]G^C[GT]G
951 CCGCGG
NspI 248 ACATGC [GA]CATG^[CT]
1124 GCATGT
PaeR7I 76 CTCGAG C^TCGAG
1264 CTCGAG
RsaI 381 GTAC GT^AC
777 GTAC
XhoI 76 CTCGAG C^TCGAG
1264 CTCGAG
XhoII 263 AGATCT [GA]^GATC[CT]
314 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AcyI AluI AvaI
BanI BanII BbeI BbvI BcnI
BglI BmyI BsaHI BsaJI BseRI
BsiEI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BssKI BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DpnII DsaI EaeI EcoRII FauI
Fnu4HI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI MboI MboII MnlI
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
SfoI SmlI TaqI TseI TthHB8I