Restriction map for
pF1KB9431
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BalI BamHI BbeI BbrPI
BciVI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BseAI
BsiWI BsmI BspEI BspHI BspLU11I
BsrDI BssHII BssSI Bst1107I BstBI
BstXI BstZ17I Bsu36I CelII DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FseI FspI HaeII HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MluNI MroI MscI MseI
MunI NarI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tru9I Tsp509I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 1258 GTCTAC GT^[AC][GT]AC
AgeI 839 ACCGGT A^CCGGT
AlwI 426 GATCC GGATC(4/5)
ApaI 1407 GGGCCC GGGCC^C
AspI 808 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 842 GGTCC G^G[AT]CC
BanI 1327 GGCACC G^G[CT][GA]CC
BbvCI 1039 GCTGAGG CCTCAGC(-5/-2)
BcgI 1109 GCAACAAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 144 TGATCA T^GATCA
BglI 1096 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 1168 CTGGAG CTGGAG(16/14)
BsaWI 839 ACCGGT [AT]^CCGG[AT]
BsmBI 66 CGTCTC CGTCTC(1/5)
Bsp1407I 1220 TGTACA T^GTACA
BspDI 1087 ATCGAT AT^CGAT
BsrGI 1220 TGTACA T^GTACA
BstYI 425 AGATCC [GA]^GATC[CT]
CfoI 1431 GCGC GCG^C
ClaI 1087 ATCGAT AT^CGAT
CpoI 841 CGGTCCG CG^G[AT]CCG
DrdI 676 GACGGGACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 429 CCTTATTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 144 TGATCA T^GATCA
HhaI 1431 GCGC GCG^C
HinP1I 1431 GCGC G^CGC
HindIII 1279 AAGCTT A^AGCTT
MaeII 1256 ACGT A^CGT
MflI 425 AGATCC [GA]^GATC[CT]
MslI 1422 CACCACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1026 GCCGGC GCC^GGC
NcoI 1454 CCATGG C^CATGG
NgoMIV 1026 GCCGGC G^CCGGC
PflFI 808 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 29 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 839 ACCGGT A^CCGGT
PvuII 601 CAGCTG CAG^CTG
RsrII 841 CGGTCCG CG^G[AT]CCG
SapI 595 GAAGAGC GCTCTTC(1/4)
SexAI 512 ACCAGGT A^CC[AT]GGT
SmaI 59 CCCGGG CCC^GGG
SspBI 1220 TGTACA T^GTACA
StuI 1079 AGGCCT AGG^CCT
TfiI 997 GATTC G^A[AT]TC
Tth111I 808 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 29 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1157 CCATCAGGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 425 AGATCC [GA]^GATC[CT]
XmaCI 59 CCCGGG C^CCGGG
XmaI 59 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 403 CGCG CG^CG
811 CGCG
AspHI 1237 GAGCTC G[AT]GC[AT]^C
1567 GAGCTC
AvaI 59 CCCGGG C^[CT]CG[GA]G
1303 CTCGGG
BsaI 1450 GAGACC GGTCTC(1/5)
684 GGTCTC
BsgI 228 CTGCAC GTGCAG(16/14)
1006 GTGCAG
BsiEI 807 CGACCG CG[GA][CT]^CG
1132 CGGTCG
BsiHKAI 1237 GAGCTC G[AT]GC[AT]^C
1567 GAGCTC
BsoBI 59 CCCGGG C^[CT]CG[GA]G
1303 CTCGGG
BspMI 112 ACCTGC ACCTGC(4/8)
343 ACCTGC
BsrBI 1416 CCGCTC CCGCTC(-3/-3)
1129 GAGCGG
BsrFI 839 ACCGGT [GA]^CCGG[CT]
1026 GCCGGC
BstAPI 871 GCACCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1391 GCACGGGCTGC
BstEII 396 GGTCACC G^GT[ACGT]ACC
1458 GGTGACC
BstPI 396 GGTCACC G^GT[ACGT]ACC
1458 GGTGACC
BstUI 403 CGCG CG^CG
811 CGCG
Cfr10I 839 ACCGGT [GA]^CCGG[CT]
1026 GCCGGC
DpnII 145 GATC ^GATC
426 GATC
DsaI 41 CCGTGG C^C[GA][CT]GG
1454 CCATGG
EcoO65I 396 GGTCACC G^GT[ACGT]ACC
1458 GGTGACC
HincII 151 GTCAAC GT[CT]^[GA]AC
274 GTCAAC
HindII 151 GTCAAC GT[CT]^[GA]AC
274 GTCAAC
MboI 145 GATC ^GATC
426 GATC
MvnI 403 CGCG CG^CG
811 CGCG
NdeII 145 GATC ^GATC
426 GATC
PstI 379 CTGCAG CTGCA^G
700 CTGCAG
SacI 1237 GAGCTC GAGCT^C
1567 GAGCTC
Sau3AI 145 GATC ^GATC
426 GATC
TaqI 1088 TCGA T^CGA
1118 TCGA
TthHB8I 1088 TCGA T^CGA
1118 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwNI
BanII BbsI BbvI BcnI BmyI
BpuAI BsaHI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DraII EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI Hin1I HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboII MnlI MspA1I MspI MvaI
MwoI NciI NlaIII NlaIV PleI
RsaI Sau96I ScrFI SfaNI SfcI
StyI TseI Tsp45I TspRI