################################## # NO CUT ENZYMES ( 197 enzymes) # ################################## AatII Acc65I AccI AccII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FokI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HincII HindII HpaI ItaI KasI KpnI KspI MaeI MaeII MaeIII MamI MfeI MluI MluNI MscI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TaqI TseI Tsp45I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccIII 38 TCCGGA T^CCGGA AcsI 441 AAATTT [GA]^AATT[CT] AocI 336 CCTTAGG CC^T[ACGT]AGG ApoI 441 AAATTT [GA]^AATT[CT] Asp700I 286 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 36 GGTCC G^G[AT]CC BbsI 397 GAAGAC GAAGAC(2/6) BglII 429 AGATCT A^GATCT BpuAI 397 GAAGAC GAAGAC(2/6) BsaJI 361 CCACGG C^C[ACGT][ACGT]GG BsaWI 38 TCCGGA [AT]^CCGG[AT] BseAI 38 TCCGGA T^CCGGA BsiYI 39 CCGGATAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 39 CCGGATAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 190 GGGAC GGGAC(10/14) BspEI 38 TCCGGA T^CCGGA BssKI 295 CCTGG ^CC[ACGT]GG BstNI 295 CCTGG CC^[AT]GG BstYI 429 AGATCT [GA]^GATC[CT] Bsu36I 336 CCTTAGG CC^T[ACGT]AGG Cac8I 61 GCTCGC GC[ACGT]^[ACGT]GC Cfr13I 36 GGTCC G^G[ACGT]CC DpnII 430 GATC ^GATC DsaI 361 CCACGG C^C[GA][CT]GG EarI 163 GAAGAG CTCTTC(1/4) Eco81I 336 CCTTAGG CC^T[ACGT]AGG EcoRII 295 CCTGG ^CC[AT]GG HapII 39 CCGG C^CGG HinfI 3 GAATC G^A[ACGT]TC HpaII 39 CCGG C^CGG HphI 168 GGTGA GGTGA(8/7) Ksp632I 163 GAAGAG CTCTTC(1/4) MboI 430 GATC ^GATC MflI 429 AGATCT [GA]^GATC[CT] MroI 38 TCCGGA T^CCGGA MslI 282 CACAGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 98 CAGCTG C[AC]G^C[GT]G MspI 39 CCGG C^CGG MvaI 295 CCTGG CC^[AT]GG NdeII 430 GATC ^GATC PvuII 98 CAGCTG CAG^CTG Sau3AI 430 GATC ^GATC Sau96I 36 GGTCC G^G[ACGT]CC ScrFI 295 CCTGG CC^[ACGT]GG SfaNI 454 GCATC GCATC(5/9) SspI 300 AATATT AAT^ATT TfiI 3 GAATC G^A[AT]TC XhoII 429 AGATCT [GA]^GATC[CT] XmnI 286 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 7 enzymes) # ################################### AfaI 56 GTAC GT^AC 82 GTAC BsrI 135 ACTGG ACTGG(1/-1) 256 ACTGG DdeI 32 CTCAG C^T[ACGT]AG 337 CTTAG HindIII 112 AAGCTT A^AGCTT 224 AAGCTT NlaIII 174 CATG CATG^ 262 CATG RsaI 56 GTAC GT^AC 82 GTAC Tsp509I 252 AATT ^AATT 442 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 5 enzymes) # ######################################### AluI MboII MnlI MseI Tru9I