Restriction map for pF1KB9442
##################################
# NO CUT ENZYMES (  197 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AciI        
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbvCI       BbvI        
BcgI        BciVI       BclI        BcnI        BfaI        
BfrI        BglI        BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstF5I      BstPI       BstUI       BstXI       
BstZ17I     CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
Fnu4HI      FokI        FseI        FspI        HaeII       
HaeIII      HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HpaI        ItaI        KasI        
KpnI        KspI        MaeI        MaeII       MaeIII      
MamI        MfeI        MluI        MluNI       MscI        
MunI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NlaIV       NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       StuI        StyI        SwaI        
TaqI        TseI        Tsp45I      TspRI       Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccIII         38  TCCGGA               T^CCGGA
AcsI          441  AAATTT               [GA]^AATT[CT]
AocI          336  CCTTAGG              CC^T[ACGT]AGG
ApoI          441  AAATTT               [GA]^AATT[CT]
Asp700I       286  GAAATGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII          36  GGTCC                G^G[AT]CC
BbsI          397  GAAGAC               GAAGAC(2/6)
BglII         429  AGATCT               A^GATCT
BpuAI         397  GAAGAC               GAAGAC(2/6)
BsaJI         361  CCACGG               C^C[ACGT][ACGT]GG
BsaWI          38  TCCGGA               [AT]^CCGG[AT]
BseAI          38  TCCGGA               T^CCGGA
BsiYI          39  CCGGATAGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           39  CCGGATAGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         190  GGGAC                GGGAC(10/14)
BspEI          38  TCCGGA               T^CCGGA
BssKI         295  CCTGG                ^CC[ACGT]GG
BstNI         295  CCTGG                CC^[AT]GG
BstYI         429  AGATCT               [GA]^GATC[CT]
Bsu36I        336  CCTTAGG              CC^T[ACGT]AGG
Cac8I          61  GCTCGC               GC[ACGT]^[ACGT]GC
Cfr13I         36  GGTCC                G^G[ACGT]CC
DpnII         430  GATC                 ^GATC
DsaI          361  CCACGG               C^C[GA][CT]GG
EarI          163  GAAGAG               CTCTTC(1/4)
Eco81I        336  CCTTAGG              CC^T[ACGT]AGG
EcoRII        295  CCTGG                ^CC[AT]GG
HapII          39  CCGG                 C^CGG
HinfI           3  GAATC                G^A[ACGT]TC
HpaII          39  CCGG                 C^CGG
HphI          168  GGTGA                GGTGA(8/7)
Ksp632I       163  GAAGAG               CTCTTC(1/4)
MboI          430  GATC                 ^GATC
MflI          429  AGATCT               [GA]^GATC[CT]
MroI           38  TCCGGA               T^CCGGA
MslI          282  CACAGAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I         98  CAGCTG               C[AC]G^C[GT]G
MspI           39  CCGG                 C^CGG
MvaI          295  CCTGG                CC^[AT]GG
NdeII         430  GATC                 ^GATC
PvuII          98  CAGCTG               CAG^CTG
Sau3AI        430  GATC                 ^GATC
Sau96I         36  GGTCC                G^G[ACGT]CC
ScrFI         295  CCTGG                CC^[ACGT]GG
SfaNI         454  GCATC                GCATC(5/9)
SspI          300  AATATT               AAT^ATT
TfiI            3  GAATC                G^A[AT]TC
XhoII         429  AGATCT               [GA]^GATC[CT]
XmnI          286  GAAATGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

AfaI           56  GTAC                 GT^AC
               82  GTAC                
BsrI          135  ACTGG                ACTGG(1/-1)
              256  ACTGG               
DdeI           32  CTCAG                C^T[ACGT]AG
              337  CTTAG               
HindIII       112  AAGCTT               A^AGCTT
              224  AAGCTT              
NlaIII        174  CATG                 CATG^
              262  CATG                
RsaI           56  GTAC                 GT^AC
               82  GTAC                
Tsp509I       252  AATT                 ^AATT
              442  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (    5 enzymes) #
#########################################

AluI        MboII       MnlI        MseI        Tru9I       
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