################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## AatII Acc65I AccIII AgeI AhdI AocI ApaI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BbeI BbrPI BbvCI BcgI BciVI BcnI BlnI BlpI Bpu1102I BsaAI BseAI BsiEI BsiWI BspDI BspEI BspHI BsrBI BssHII BssSI BstAPI BstBI Bsu36I CelII ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoT22I FauI FseI FspI KasI KpnI KspI MluI MroI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI PvuI RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccII 24 CGCG CG^CG AclI 3261 AACGTT AA^CGTT AcyI 2715 GGCGTC G[GA]^CG[CT]C AflII 2736 CTTAAG C^TTAAG AlwNI 797 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 51 AGCGCT AGC^GCT Asp700I 3152 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 1816 GGATCC G^GATCC BclI 888 TGATCA T^GATCA BfrI 2736 CTTAAG C^TTAAG BglI 1634 GCCCCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 2824 AGATCT A^GATCT BsaHI 2715 GGCGTC G[GA]^CG[CT]C BsaWI 121 TCCGGT [AT]^CCGG[AT] BsmBI 2717 CGTCTC CGTCTC(1/5) BsrDI 411 GCAATG GCAATG(2/0) BstEII 1450 GGTGACC G^GT[ACGT]ACC BstPI 1450 GGTGACC G^GT[ACGT]ACC BstUI 24 CGCG CG^CG Eco47III 51 AGCGCT AGC^GCT EcoO65I 1450 GGTGACC G^GT[ACGT]ACC EcoRI 1743 GAATTC G^AATTC EcoRV 3064 GATATC GAT^ATC FbaI 888 TGATCA T^GATCA HaeII 51 AGCGCT [GA]GCGC^[CT] HgaI 2716 GCGTC GACGC(5/10) Hin1I 2715 GGCGTC G[GA]^CG[CT]C HindIII 1973 AAGCTT A^AGCTT HpaI 2466 GTTAAC GTT^AAC MfeI 1670 CAATTG C^AATTG MunI 1670 CAATTG C^AATTG MvnI 24 CGCG CG^CG PflMI 3230 CCAGAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 1577 GAGTC GAGTC(4/5) Psp1406I 3261 AACGTT AA^CGTT SacI 3532 GAGCTC GAGCT^C ScaI 1847 AGTACT AGT^ACT SspI 114 AATATT AAT^ATT StuI 600 AGGCCT AGG^CCT SwaI 1880 ATTTAAAT ATTT^AAAT TaqI 1656 TCGA T^CGA TthHB8I 1656 TCGA T^CGA Van91I 3230 CCAGAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 3152 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AflIII 271 ACATGT A^C[GA][CT]GT 388 ACATGT Alw44I 1535 GTGCAC G^TGCAC 2881 GTGCAC ApaLI 1535 GTGCAC G^TGCAC 2881 GTGCAC BalI 1242 TGGCCA TGG^CCA 2081 TGGCCA BanI 1915 GGTGCC G^G[CT][GA]CC 2168 GGTGCC BanII 2236 GAGCCC G[GA]GC[CT]^C 3532 GAGCTC BbsI 1230 GAAGAC GAAGAC(2/6) 2338 GTCTTC BpuAI 1230 GAAGAC GAAGAC(2/6) 2338 GTCTTC BsaBI 2117 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3064 GATATCAATC BsaI 3523 GAGACC GGTCTC(1/5) 97 GGTCTC BseRI 1367 CTCCTC GAGGAG(10/8) 1109 GAGGAG BsmI 2013 GAATGC GAATGC(1/-1) 305 GCATTC Bsp1407I 594 TGTACA T^GTACA 1544 TGTACA BspLU11I 271 ACATGT A^CATGT 388 ACATGT BspMI 489 ACCTGC ACCTGC(4/8) 3175 ACCTGC BsrFI 1384 ACCGGC [GA]^CCGG[CT] 2568 GCCGGT BsrGI 594 TGTACA T^GTACA 1544 TGTACA Bst1107I 1756 GTATAC GTA^TAC 2985 GTATAC BstYI 1816 GGATCC [GA]^GATC[CT] 2824 AGATCT BstZ17I 1756 GTATAC GTA^TAC 2985 GTATAC CfoI 25 GCGC GCG^C 52 GCGC Cfr10I 1384 ACCGGC [GA]^CCGG[CT] 2568 GCCGGT HhaI 25 GCGC GCG^C 52 GCGC HinP1I 25 GCGC G^CGC 52 GCGC MamI 2117 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3064 GATATCAATC MflI 1816 GGATCC [GA]^GATC[CT] 2824 AGATCT MluNI 1242 TGGCCA TGG^CCA 2081 TGGCCA MscI 1242 TGGCCA TGG^CCA 2081 TGGCCA PpuMI 740 GGGACCT [GA]G^G[AT]CC[CT] 1858 AGGTCCT PvuII 2420 CAGCTG CAG^CTG 3287 CAGCTG SmlI 2426 CTCAAG C^T[CT][GA]AG 2736 CTTAAG SspBI 594 TGTACA T^GTACA 1544 TGTACA XcmI 172 CCAAAAGGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2355 CCACAAAGACTGTGG XhoII 1816 GGATCC [GA]^GATC[CT] 2824 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI ApoI AspHI AvaI AvaII BbvI BfaI BmyI BpmI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstXI Cac8I Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PshAI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI