Restriction map for
pF1KB9470
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII ApoI AscI
AseI AsnI Asp700I Asp718I AspI
BamHI BbrPI BbsI BciVI BclI
BfrI BglII BpuAI BsaAI BsaBI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrGI Bst1107I
BstBI BstZ17I ClaI CpoI DraI
DrdI EcoRI EcoRV EcoT22I FbaI
FseI HgaI HpaI KpnI KspI
MamI MfeI MluI MroI MseI
MslI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI ScaI
SfuI SgrAI SnaBI SpeI SspBI
SspI SwaI Tru9I Tth111I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 68 enzymes) #
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AcyI 384 GGCGCC G[GA]^CG[CT]C
AgeI 924 ACCGGT A^CCGGT
AhdI 1549 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 541 GTGCAC G^TGCAC
AocI 700 CCTCAGG CC^T[ACGT]AGG
Aor51HI 167 AGCGCT AGC^GCT
ApaLI 541 GTGCAC G^TGCAC
AspEI 1549 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 967 TGCGCA TGC^GCA
AvrII 1920 CCTAGG C^CTAGG
BalI 1391 TGGCCA TGG^CCA
BbeI 384 GGCGCC GGCGC^C
BbvCI 624 CCTCAGC CCTCAGC(-5/-2)
BlnI 1920 CCTAGG C^CTAGG
BlpI 3 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3 GCTGAGC GC^T[ACGT]AGC
BsaHI 384 GGCGCC G[GA]^CG[CT]C
BsaI 1301 GAGACC GGTCTC(1/5)
BsaWI 924 ACCGGT [AT]^CCGG[AT]
BsiEI 1067 CGGCCG CG[GA][CT]^CG
BspHI 149 TCATGA T^CATGA
BsrDI 963 GCAATG GCAATG(2/0)
BssHII 1648 GCGCGC G^CGCGC
BssSI 358 CACGAG CACGAG(-5/-1)
BstEII 993 GGTCACC G^GT[ACGT]ACC
BstPI 993 GGTCACC G^GT[ACGT]ACC
BstXI 1553 CCAAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 323 GGATCT [GA]^GATC[CT]
Bsu36I 700 CCTCAGG CC^T[ACGT]AGG
CelII 3 GCTGAGC GC^T[ACGT]AGC
DraIII 544 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1067 CGGCCG C^GGCCG
Eam1105I 1549 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1067 CGGCCG C^GGCCG
Eco47III 167 AGCGCT AGC^GCT
Eco52I 1067 CGGCCG C^GGCCG
Eco81I 700 CCTCAGG CC^T[ACGT]AGG
EcoO65I 993 GGTCACC G^GT[ACGT]ACC
FspI 967 TGCGCA TGC^GCA
Hin1I 384 GGCGCC G[GA]^CG[CT]C
HincII 769 GTCAAC GT[CT]^[GA]AC
HindII 769 GTCAAC GT[CT]^[GA]AC
HindIII 1926 AAGCTT A^AGCTT
KasI 384 GGCGCC G^GCGCC
MaeII 539 ACGT A^CGT
MflI 323 GGATCT [GA]^GATC[CT]
MluNI 1391 TGGCCA TGG^CCA
MscI 1391 TGGCCA TGG^CCA
NarI 384 GGCGCC GG^CGCC
NspI 455 GCATGC [GA]CATG^[CT]
PinAI 924 ACCGGT A^CCGGT
PstI 487 CTGCAG CTGCA^G
RcaI 149 TCATGA T^CATGA
SapI 912 GCTCTTC GCTCTTC(1/4)
SbfI 486 CCTGCAGG CCTGCA^GG
SexAI 473 ACCTGGT A^CC[AT]GGT
SfcI 487 CTGCAG C^T[GA][CT]AG
SfiI 24 GGCCAGGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 384 GGCGCC GGC^GCC
SmaI 1637 CCCGGG CCC^GGG
SmlI 1309 CTCAAG C^T[CT][GA]AG
SphI 455 GCATGC GCATG^C
Sse8387I 486 CCTGCAGG CCTGCA^GG
StuI 1661 AGGCCT AGG^CCT
Tsp509I 709 AATT ^AATT
XhoII 323 GGATCT [GA]^GATC[CT]
XmaCI 1637 CCCGGG C^CCGGG
XmaI 1637 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AlwI 189 GGATC GGATC(4/5)
323 GGATC
BcgI 1476 CGACTCGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
860 GCACTTCCCTCG
BglI 25 GCCAGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1393 GCCAGTATGGC
BsmAI 1301 GAGAC GTCTC(1/5)
1332 GAGAC
EarI 913 CTCTTC CTCTTC(1/4)
1050 CTCTTC
EcoNI 602 CCTTCGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
696 CCTTCCTCAGG
Ksp632I 913 CTCTTC CTCTTC(1/4)
1050 CTCTTC
PflMI 786 CCAGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1572 CCACAGCATGG
SacI 580 GAGCTC GAGCT^C
1684 GAGCTC
Van91I 786 CCAGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1572 CCACAGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
ApaI AspHI AvaI AvaII BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I