Restriction map for
pF1KB9472
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# NO CUT ENZYMES ( 112 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AocI ApoI
AscI AseI AsnI Asp700I Asp718I
AvrII BamHI BcgI BciVI BclI
BfrI BglII BlnI BsaBI BsaWI
BseAI BsgI BsiWI BsmBI BsmI
BspEI BspHI BspLU11I BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I Bsu36I CpoI
DraI EarI Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HindIII HinfI HpaI KpnI Ksp632I
KspI MamI MfeI MflI MluI
MroI MseI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PleI PmeI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI SwaI TfiI Tru9I
Tsp509I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AhdI 928 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1215 GTGCAC G^TGCAC
Aor51HI 1315 AGCGCT AGC^GCT
ApaLI 1215 GTGCAC G^TGCAC
AspEI 928 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 786 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 74 CCCGAG C^[CT]CG[GA]G
AviII 809 TGCGCA TGC^GCA
BalI 518 TGGCCA TGG^CCA
BbsI 1162 GTCTTC GAAGAC(2/6)
BbvCI 1311 CCTCAGC CCTCAGC(-5/-2)
BfaI 1118 CTAG C^TAG
BglI 240 GCCGCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 589 GCTGAGC GC^T[ACGT]AGC
BpmI 219 CTGGAG CTGGAG(16/14)
Bpu1102I 589 GCTGAGC GC^T[ACGT]AGC
BpuAI 1162 GTCTTC GAAGAC(2/6)
BsaI 1352 GGTCTC GGTCTC(1/5)
BsoBI 74 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1004 TGTACA T^GTACA
BspDI 886 ATCGAT AT^CGAT
BsrBI 268 GAGCGG CCGCTC(-3/-3)
BsrGI 1004 TGTACA T^GTACA
CelII 589 GCTGAGC GC^T[ACGT]AGC
ClaI 886 ATCGAT AT^CGAT
DraIII 754 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 97 GACAAGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 928 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1315 AGCGCT AGC^GCT
FspI 809 TGCGCA TGC^GCA
HincII 328 GTCAAC GT[CT]^[GA]AC
HindII 328 GTCAAC GT[CT]^[GA]AC
MaeI 1118 CTAG C^TAG
MaeIII 926 GTGAC ^GT[ACGT]AC
MluNI 518 TGGCCA TGG^CCA
MscI 518 TGGCCA TGG^CCA
NcoI 1028 CCATGG C^CATGG
NspI 1001 GCATGT [GA]CATG^[CT]
PflFI 786 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1390 CCAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 145 CTGCAG CTGCA^G
SmlI 508 CTCAAG C^T[CT][GA]AG
SspBI 1004 TGTACA T^GTACA
StuI 566 AGGCCT AGG^CCT
Tsp45I 926 GTGAC ^GT[GC]AC
Tth111I 786 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1390 CCAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 714 GATCC GGATC(4/5)
831 GATCC
ApaI 382 GGGCCC GGGCC^C
1380 GGGCCC
AspHI 739 GTGCTC G[AT]GC[AT]^C
1215 GTGCAC
BbrPI 546 CACGTG CAC^GTG
1471 CACGTG
BsiHKAI 739 GTGCTC G[AT]GC[AT]^C
1215 GTGCAC
BsmAI 1283 GAGAC GTCTC(1/5)
1353 GTCTC
BstXI 884 CCATCGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1022 CCAACACCATGG
EagI 62 CGGCCG C^GGCCG
634 CGGCCG
EclXI 62 CGGCCG C^GGCCG
634 CGGCCG
Eco52I 62 CGGCCG C^GGCCG
634 CGGCCG
NaeI 280 GCCGGC GCC^GGC
1153 GCCGGC
NgoMIV 280 GCCGGC G^CCGGC
1153 GCCGGC
PmaCI 546 CACGTG CAC^GTG
1471 CACGTG
PmlI 546 CACGTG CAC^GTG
1471 CACGTG
SfcI 145 CTGCAG C^T[GA][CT]AG
1035 CTACAG
TaqI 670 TCGA T^CGA
887 TCGA
TthHB8I 670 TCGA T^CGA
887 TCGA
XcmI 695 CCACCTACAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
881 CCACCATCGATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwNI AvaII BanI BanII
BbeI BbvI BcnI BmyI BsaAI
BsaHI BsaJI BseRI BsiEI BsiYI
BslI BsmFI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII HphI ItaI
KasI MaeII MboI MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SfoI StyI TseI
TspRI