Restriction map for
pF1KB9474
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# NO CUT ENZYMES ( 171 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmFI BsmI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DdeI DraI
DraIII DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRV EcoT22I FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MamI MfeI MflI MluI
MluNI MroI MscI MseI MspA1I
MunI NarI NcoI NdeI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TaqI
TfiI Tru9I Tth111I TthHB8I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AcsI 9 GAATTC [GA]^AATT[CT]
AgeI 232 ACCGGT A^CCGGT
AluI 446 AGCT AG^CT
ApoI 9 GAATTC [GA]^AATT[CT]
AvaI 267 CTCGGG C^[CT]CG[GA]G
AvaII 272 GGTCC G^G[AT]CC
BbsI 324 GAAGAC GAAGAC(2/6)
BcnI 423 CCCGG CC^[GC]GG
BfaI 283 CTAG C^TAG
BpuAI 324 GAAGAC GAAGAC(2/6)
BsaWI 232 ACCGGT [AT]^CCGG[AT]
BseRI 132 CTCCTC GAGGAG(10/8)
BsiYI 168 CCACTACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 168 CCACTACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 267 CTCGGG C^[CT]CG[GA]G
BstEII 225 GGTCACC G^GT[ACGT]ACC
BstPI 225 GGTCACC G^GT[ACGT]ACC
BstXI 251 CCAAAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 29 CGGCCA [CT]^GGCC[GA]
EcoO65I 225 GGTCACC G^GT[ACGT]ACC
EcoRI 9 GAATTC G^AATTC
EcoT14I 47 CCAAGG C^C[AT][AT]GG
FauI 42 GCGGG CCCGC(4/6)
HaeII 218 AGCGCC [GA]GCGC^[CT]
HinfI 193 GACTC G^A[ACGT]TC
MaeI 283 CTAG C^TAG
MaeII 16 ACGT A^CGT
MaeIII 226 GTCAC ^GT[ACGT]AC
MslI 90 CATGATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 568 GCCGGC GCC^GGC
NciI 423 CCCGG CC^[GC]GG
NgoMIV 568 GCCGGC G^CCGGC
NheI 282 GCTAGC G^CTAGC
NlaIII 90 CATG CATG^
PinAI 232 ACCGGT A^CCGGT
PleI 193 GACTC GAGTC(4/5)
PpuMI 271 GGGTCCT [GA]G^G[AT]CC[CT]
SgrAI 231 CACCGGTG C[GA]^CCGG[CT]G
SmlI 241 CTCAAG C^T[CT][GA]AG
StuI 50 AGGCCT AGG^CCT
StyI 47 CCAAGG C^C[AT][AT]GG
Tsp45I 226 GTCAC ^GT[GC]AC
Tsp509I 10 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AccII 124 CGCG CG^CG
137 CGCG
BstNI 108 CCAGG CC^[AT]GG
222 CCTGG
BstUI 124 CGCG CG^CG
137 CGCG
DpnII 93 GATC ^GATC
201 GATC
DraII 262 GGGGCCT [GA]G^G[ACGT]CC[CT]
271 GGGTCCT
EcoO109I 262 GGGGCCT [GA]G^G[ACGT]CC[CT]
271 GGGTCCT
EcoRII 108 CCAGG ^CC[AT]GG
222 CCTGG
HphI 181 GGTGA GGTGA(8/7)
227 TCACC
MboI 93 GATC ^GATC
201 GATC
MvaI 108 CCAGG CC^[AT]GG
222 CCTGG
MvnI 124 CGCG CG^CG
137 CGCG
NdeII 93 GATC ^GATC
201 GATC
Sau3AI 93 GATC ^GATC
201 GATC
SfaNI 367 GCATC GCATC(5/9)
508 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI BbvI BglI BsaJI BsrFI
BsrI BssKI BstF5I Cac8I CfoI
Cfr10I Cfr13I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII ItaI
MboII MnlI MspI MwoI NlaIV
Sau96I ScrFI TseI TspRI XcmI