Restriction map for
pF1KB9494
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# NO CUT ENZYMES ( 90 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI AocI Aor51HI
AscI AseI AsnI Asp718I AspEI
AvrII BamHI BcgI BciVI BfrI
BglII BlnI BsaBI BseAI BsiWI
BsmBI BsmI BspDI BspEI BsrDI
Bst1107I BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
Eam1105I Eco47III Eco81I EcoO65I EcoRV
EcoT22I FseI HindIII HpaI KpnI
KspI MamI MluI MroI MseI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SfiI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI TfiI
Tru9I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AflIII 602 ACATGT A^C[GA][CT]GT
Alw44I 2389 GTGCAC G^TGCAC
ApaLI 2389 GTGCAC G^TGCAC
Asp700I 1842 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 106 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2497 TGCGCA TGC^GCA
BbrPI 1528 CACGTG CAC^GTG
BbsI 264 GAAGAC GAAGAC(2/6)
BbvCI 1115 CCTCAGC CCTCAGC(-5/-2)
BclI 1832 TGATCA T^GATCA
BfaI 521 CTAG C^TAG
BpuAI 264 GAAGAC GAAGAC(2/6)
BsaI 2406 GGTCTC GGTCTC(1/5)
BsaWI 2087 TCCGGT [AT]^CCGG[AT]
BspHI 1835 TCATGA T^CATGA
BspLU11I 602 ACATGT A^CATGT
BssHII 56 GCGCGC G^CGCGC
BssSI 1288 CACGAG CACGAG(-5/-1)
BstBI 142 TTCGAA TT^CGAA
DraIII 211 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 311 CCTGGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1832 TGATCA T^GATCA
FspI 2497 TGCGCA TGC^GCA
HincII 2194 GTCAAC GT[CT]^[GA]AC
HindII 2194 GTCAAC GT[CT]^[GA]AC
MaeI 521 CTAG C^TAG
MfeI 611 CAATTG C^AATTG
MunI 611 CAATTG C^AATTG
NspV 142 TTCGAA TT^CGAA
PflFI 106 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1528 CACGTG CAC^GTG
PmlI 1528 CACGTG CAC^GTG
PpuMI 334 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 1835 TCATGA T^CATGA
SacI 631 GAGCTC GAGCT^C
SapI 1164 GCTCTTC GCTCTTC(1/4)
SexAI 1826 ACCAGGT A^CC[AT]GGT
SfuI 142 TTCGAA TT^CGAA
SmlI 1780 CTCAAG C^T[CT][GA]AG
Tth111I 106 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2126 CCATCGGGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1842 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcsI 775 GAATTC [GA]^AATT[CT]
1993 GAATTC
ApaI 136 GGGCCC GGGCC^C
1508 GGGCCC
ApoI 775 GAATTC [GA]^AATT[CT]
1993 GAATTC
BalI 477 TGGCCA TGG^CCA
1532 TGGCCA
BbeI 164 GGCGCC GGCGC^C
2509 GGCGCC
BlpI 1770 GCTGAGC GC^T[ACGT]AGC
2433 GCTGAGC
Bpu1102I 1770 GCTGAGC GC^T[ACGT]AGC
2433 GCTGAGC
BsaAI 1375 CACGTA [CT]AC^GT[GA]
1528 CACGTG
BsmAI 254 GAGAC GTCTC(1/5)
2407 GTCTC
BsrFI 342 ACCGGC [GA]^CCGG[CT]
2261 ACCGGC
CelII 1770 GCTGAGC GC^T[ACGT]AGC
2433 GCTGAGC
Cfr10I 342 ACCGGC [GA]^CCGG[CT]
2261 ACCGGC
EarI 763 CTCTTC CTCTTC(1/4)
1165 CTCTTC
EcoRI 775 GAATTC G^AATTC
1993 GAATTC
HgaI 123 GACGC GACGC(5/10)
1192 GACGC
KasI 164 GGCGCC G^GCGCC
2509 GGCGCC
Ksp632I 763 CTCTTC CTCTTC(1/4)
1165 CTCTTC
MluNI 477 TGGCCA TGG^CCA
1532 TGGCCA
MscI 477 TGGCCA TGG^CCA
1532 TGGCCA
NarI 164 GGCGCC GG^CGCC
2509 GGCGCC
NcoI 531 CCATGG C^CATGG
863 CCATGG
PflMI 1446 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1926 CCAGCCAGTGG
SfoI 164 GGCGCC GGC^GCC
2509 GGCGCC
StuI 1712 AGGCCT AGG^CCT
2153 AGGCCT
Van91I 1446 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1926 CCAGCCAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BanI BanII BbvI BcnI BglI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrBI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EclXI Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspBI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII