Restriction map for
pF1KB9499
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# NO CUT ENZYMES ( 91 enzymes) #
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Acc65I AccIII AclI AflII AflIII
AgeI AhdI Alw44I ApaLI AscI
Asp718I AspEI AviII AvrII BamHI
BbeI BbrPI BcgI BciVI BclI
BfrI BlnI BsaAI BsaBI BseAI
BsiEI BsiWI BspEI BspHI BspLU11I
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I CpoI DraI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI NarI
NdeI NotI NruI NsiI NspI
NspV PacI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
XbaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 2364 GACGTC GACGT^C
AccII 1090 CGCG CG^CG
AcyI 2364 GACGTC G[GA]^CG[CT]C
AocI 2582 CCTCAGG CC^T[ACGT]AGG
Aor51HI 3175 AGCGCT AGC^GCT
AseI 1162 ATTAAT AT^TAAT
AsnI 1162 ATTAAT AT^TAAT
AspI 3003 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 1123 GCTGAGG CCTCAGC(-5/-2)
BglI 1674 GCCCCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 255 AGATCT A^GATCT
BsaHI 2364 GACGTC G[GA]^CG[CT]C
BsaI 3201 GGTCTC GGTCTC(1/5)
BsaWI 1466 ACCGGA [AT]^CCGG[AT]
BsmBI 2366 CGTCTC CGTCTC(1/5)
BsmI 1396 GAATGC GAATGC(1/-1)
Bsp1407I 2131 TGTACA T^GTACA
BspMI 2103 ACCTGC ACCTGC(4/8)
BsrBI 1218 CCGCTC CCGCTC(-3/-3)
BsrDI 1860 CATTGC GCAATG(2/0)
BsrGI 2131 TGTACA T^GTACA
BstUI 1090 CGCG CG^CG
Bsu36I 2582 CCTCAGG CC^T[ACGT]AGG
DraIII 2502 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2964 GACCTCGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3175 AGCGCT AGC^GCT
Eco81I 2582 CCTCAGG CC^T[ACGT]AGG
HgaI 1243 GCGTC GACGC(5/10)
Hin1I 2364 GACGTC G[GA]^CG[CT]C
MvnI 1090 CGCG CG^CG
NheI 2185 GCTAGC G^CTAGC
PaeR7I 1908 CTCGAG C^TCGAG
PflFI 3003 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1162 ATTAAT AT^TAAT
SapI 2750 GCTCTTC GCTCTTC(1/4)
SmaI 3235 CCCGGG CCC^GGG
SspBI 2131 TGTACA T^GTACA
SspI 713 AATATT AAT^ATT
Tth111I 3003 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1908 CTCGAG C^TCGAG
XmaCI 3235 CCCGGG C^CCGGG
XmaI 3235 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccI 1806 GTCTAC GT^[AC][GT]AC
3009 GTCTAC
AlwI 265 GATCC GGATC(4/5)
1995 GATCC
ApaI 2374 GGGCCC GGGCC^C
2484 GGGCCC
BfaI 2162 CTAG C^TAG
2186 CTAG
BlpI 1203 GCTGAGC GC^T[ACGT]AGC
2341 GCTAAGC
Bpu1102I 1203 GCTGAGC GC^T[ACGT]AGC
2341 GCTAAGC
BsgI 2087 CTGCAC GTGCAG(16/14)
2570 CTGCAC
BspDI 376 ATCGAT AT^CGAT
415 ATCGAT
BstAPI 1417 GCAAAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3342 GCAGAGTCTGC
BstYI 255 AGATCT [GA]^GATC[CT]
264 AGATCC
CelII 1203 GCTGAGC GC^T[ACGT]AGC
2341 GCTAAGC
ClaI 376 ATCGAT AT^CGAT
415 ATCGAT
EcoNI 1907 CCTCGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2304 CCTCAATAAGG
EcoRI 1036 GAATTC G^AATTC
2074 GAATTC
HaeII 3175 AGCGCT [GA]GCGC^[CT]
3303 AGCGCC
HincII 1963 GTCAAC GT[CT]^[GA]AC
2889 GTTGAC
HindII 1963 GTCAAC GT[CT]^[GA]AC
2889 GTTGAC
MaeI 2162 CTAG C^TAG
2186 CTAG
MaeII 348 ACGT A^CGT
2365 ACGT
MflI 255 AGATCT [GA]^GATC[CT]
264 AGATCC
MslI 915 CACTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1297 CACCTCTATG
PvuII 128 CAGCTG CAG^CTG
2060 CAGCTG
SacI 1918 GAGCTC GAGCT^C
3114 GAGCTC
XcmI 1155 CCAGGATATTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3246 CCACCATGGCCTTGG
XhoII 255 AGATCT [GA]^GATC[CT]
264 AGATCC
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI Asp700I AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BmyI BpmI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MluNI MnlI MscI
MseI MspA1I MspI MvaI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV PflMI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XmnI