Restriction map for
pF1KB9504
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# NO CUT ENZYMES ( 82 enzymes) #
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AatII AccIII AclI AflII AgeI
AhdI AscI AseI AsnI AspEI
AspI AvrII BbrPI BciVI BclI
BfrI BglII BlnI BseAI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspLU11I BssHII BssSI Bst1107I BstBI
BstZ17I ClaI CpoI DraI EagI
Eam1105I EclXI Eco52I EcoRV EcoT22I
FbaI HpaI KspI MfeI MluI
MroI MseI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SfiI SfuI
SmaI SnaBI SpeI SphI SspI
SwaI Tru9I Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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Acc65I 2939 GGTACC G^GTACC
AccI 2485 GTCTAC GT^[AC][GT]AC
AcsI 3127 GAATTC [GA]^AATT[CT]
AflIII 2786 ACGTGT A^C[GA][CT]GT
AocI 1233 CCTGAGG CC^T[ACGT]AGG
Aor51HI 3623 AGCGCT AGC^GCT
ApaI 2888 GGGCCC GGGCC^C
ApoI 3127 GAATTC [GA]^AATT[CT]
Asp718I 2939 GGTACC G^GTACC
BamHI 2903 GGATCC G^GATCC
BbeI 2865 GGCGCC GGCGC^C
BcgI 2550 GCACGGTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 2785 TACGTG [CT]AC^GT[GA]
BsaBI 3258 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3617 ACCGGA [AT]^CCGG[AT]
BsrDI 1268 GCAATG GCAATG(2/0)
BstAPI 3721 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3396 GGTCACC G^GT[ACGT]ACC
BstPI 3396 GGTCACC G^GT[ACGT]ACC
Bsu36I 1233 CCTGAGG CC^T[ACGT]AGG
DrdI 3226 GACCTCTCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3623 AGCGCT AGC^GCT
Eco81I 1233 CCTGAGG CC^T[ACGT]AGG
EcoO65I 3396 GGTCACC G^GT[ACGT]ACC
EcoRI 3127 GAATTC G^AATTC
FseI 1338 GGCCGGCC GGCCGG^CC
HindIII 1562 AAGCTT A^AGCTT
KasI 2865 GGCGCC G^GCGCC
KpnI 2939 GGTACC GGTAC^C
MamI 3258 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 2865 GGCGCC GG^CGCC
SapI 1098 GCTCTTC GCTCTTC(1/4)
ScaI 3922 AGTACT AGT^ACT
SfoI 2865 GGCGCC GGC^GCC
SgrAI 2371 CGCCGGTG C[GA]^CCGG[CT]G
StuI 1751 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 529 CGCG CG^CG
2527 CGCG
Asp700I 2424 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3621 GAAGCGCTTC
AviII 533 TGCGCA TGC^GCA
983 TGCGCA
BglI 1811 GCCTGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2007 GCCCGGCTGGC
BsgI 88 GTGCAG GTGCAG(16/14)
3469 GTGCAG
Bsp1407I 389 TGTACA T^GTACA
2270 TGTACA
BsrGI 389 TGTACA T^GTACA
2270 TGTACA
BstUI 529 CGCG CG^CG
2527 CGCG
DraIII 800 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
1365 CACCTGGTG
EcoNI 1920 CCTAGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3054 CCTACAGCAGG
FspI 533 TGCGCA TGC^GCA
983 TGCGCA
HincII 1902 GTCAAC GT[CT]^[GA]AC
2409 GTTGAC
HindII 1902 GTCAAC GT[CT]^[GA]AC
2409 GTTGAC
MaeII 1283 ACGT A^CGT
2786 ACGT
MvnI 529 CGCG CG^CG
2527 CGCG
NcoI 1910 CCATGG C^CATGG
2846 CCATGG
PleI 2758 GACTC GAGTC(4/5)
727 GAGTC
SbfI 789 CCTGCAGG CCTGCA^GG
1344 CCTGCAGG
Sse8387I 789 CCTGCAGG CCTGCA^GG
1344 CCTGCAGG
SspBI 389 TGTACA T^GTACA
2270 TGTACA
TfiI 1169 GATTC G^A[AT]TC
2822 GAATC
XmnI 2424 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3621 GAAGCGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcyI AfaI AluI Alw44I
AlwI AlwNI ApaLI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspHI BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PpuMI PstI PvuII RcaI RsaI
SacI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmlI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII