Restriction map for
pF1KB9506
##################################
# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AocI ApaLI ApoI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BbsI BbvCI BcgI BclI BfaI
BfrI BglII BlnI BpuAI BsaAI
BsaBI BseAI BsgI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
ClaI CpoI DpnII DraI DraIII
DrdI Eam1105I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FspI
HincII HindII HindIII HpaI KpnI
MaeI MaeII MamI MboI MfeI
MflI MluI MroI MseI MunI
NdeI NdeII NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SalI Sau3AI SbfI ScaI SexAI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp509I
Tth111I XbaI XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AfaI 622 GTAC GT^AC
AlwNI 533 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 143 AGCGCT AGC^GCT
AscI 125 GGCGCGCC GG^CGCGCC
BciVI 1133 GTATCC GTATCC(6/5)
BsaWI 921 TCCGGT [AT]^CCGG[AT]
BsiEI 65 CGGCCG CG[GA][CT]^CG
BsiWI 621 CGTACG C^GTACG
BsmBI 474 CGTCTC CGTCTC(1/5)
BspMI 330 ACCTGC ACCTGC(4/8)
BsrBI 1141 GAGCGG CCGCTC(-3/-3)
BssHII 126 GCGCGC G^CGCGC
EagI 65 CGGCCG C^GGCCG
EclXI 65 CGGCCG C^GGCCG
Eco47III 143 AGCGCT AGC^GCT
Eco52I 65 CGGCCG C^GGCCG
FseI 782 GGCCGGCC GGCCGG^CC
HgaI 1283 GCGTC GACGC(5/10)
NcoI 485 CCATGG C^CATGG
PflMI 357 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 515 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 435 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 572 CTGCAG CTGCA^G
RsaI 622 GTAC GT^AC
SacI 382 GAGCTC GAGCT^C
SfaNI 1027 GCATC GCATC(5/9)
SfcI 572 CTGCAG C^T[GA][CT]AG
Van91I 357 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BalI 527 TGGCCA TGG^CCA
548 TGGCCA
BlpI 1106 GCTCAGC GC^T[ACGT]AGC
1114 GCTCAGC
Bpu1102I 1106 GCTCAGC GC^T[ACGT]AGC
1114 GCTCAGC
BseRI 816 GAGGAG GAGGAG(10/8)
1312 GAGGAG
BsrFI 43 GCCGGC [GA]^CCGG[CT]
783 GCCGGC
CelII 1106 GCTCAGC GC^T[ACGT]AGC
1114 GCTCAGC
Cfr10I 43 GCCGGC [GA]^CCGG[CT]
783 GCCGGC
HphI 432 GGTGA GGTGA(8/7)
924 GGTGA
MluNI 527 TGGCCA TGG^CCA
548 TGGCCA
MscI 527 TGGCCA TGG^CCA
548 TGGCCA
MslI 480 CACTACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
855 CACAGAGGTG
NaeI 43 GCCGGC GCC^GGC
783 GCCGGC
NgoMIV 43 GCCGGC G^CCGGC
783 GCCGGC
NlaIII 288 CATG CATG^
486 CATG
PvuII 1109 CAGCTG CAG^CTG
1117 CAGCTG
SapI 379 GAAGAGC GCTCTTC(1/4)
448 GAAGAGC
SmaI 73 CCCGGG CCC^GGG
1065 CCCGGG
XcmI 416 CCACCAGCCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
419 CCAGCCCCCTGGTGG
XmaCI 73 CCCGGG C^CCGGG
1065 CCCGGG
XmaI 73 CCCGGG C^CCGGG
1065 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AcyI AluI ApaI
AspHI AvaI AvaII BanI BanII
BbeI BbvI BcnI BglI BmyI
BpmI BsaHI BsaI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr13I
DdeI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII ItaI
KasI Ksp632I KspI MaeIII MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NarI NciI NlaIV PleI
SacII Sau96I ScrFI SfoI StyI
TaqI TseI Tsp45I TspRI TthHB8I