Restriction map for
pF1KB9536
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
AhdI AlwNI AocI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaII AviII BanII BbeI
BbrPI BbsI BbvCI BbvI BcgI
BciVI BclI BcnI BfrI BpmI
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsmFI BspDI BspEI BspHI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstUI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRV FauI FbaI
Fnu4HI FokI FseI FspI HapII
Hin1I HincII HindII HpaI HpaII
ItaI KasI KpnI KspI MaeII
MamI MfeI MluI MroI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TfiI TseI Tth111I Van91I
XbaI XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AflIII 382 ACATGT A^C[GA][CT]GT
Alw44I 766 GTGCAC G^TGCAC
Aor51HI 336 AGCGCT AGC^GCT
ApaLI 766 GTGCAC G^TGCAC
AspHI 766 GTGCAC G[AT]GC[AT]^C
AvaI 556 CTCGAG C^[CT]CG[GA]G
AvrII 993 CCTAGG C^CTAGG
BalI 744 TGGCCA TGG^CCA
BamHI 272 GGATCC G^GATCC
BfaI 994 CTAG C^TAG
BglI 443 GCCATACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 993 CCTAGG C^CTAGG
BlpI 1167 GCTTAGC GC^T[ACGT]AGC
BmyI 766 GTGCAC G[AGT]GC[ACT]^C
Bpu1102I 1167 GCTTAGC GC^T[ACGT]AGC
BseRI 1039 GAGGAG GAGGAG(10/8)
BsiHKAI 766 GTGCAC G[AT]GC[AT]^C
BsmAI 554 GTCTC GTCTC(1/5)
BsmI 139 GAATGC GAATGC(1/-1)
BsoBI 556 CTCGAG C^[CT]CG[GA]G
Bsp1286I 766 GTGCAC G[AGT]GC[ACT]^C
BspLU11I 382 ACATGT A^CATGT
BspMI 364 GCAGGT ACCTGC(4/8)
BstXI 958 CCACATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 143 GCAAGC GC[ACGT]^[ACGT]GC
CelII 1167 GCTTAGC GC^T[ACGT]AGC
CfoI 337 GCGC GCG^C
Cfr13I 626 GGCCC G^G[ACGT]CC
DraI 877 TTTAAA TTT^AAA
DraII 625 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 744 TGGCCA [CT]^GGCC[GA]
Eco47III 336 AGCGCT AGC^GCT
EcoO109I 625 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 938 GAATTC G^AATTC
EcoT14I 993 CCTAGG C^C[AT][AT]GG
EcoT22I 94 ATGCAT ATGCA^T
HaeII 336 AGCGCT [GA]GCGC^[CT]
HgaI 1183 GCGTC GACGC(5/10)
HhaI 337 GCGC GCG^C
HinP1I 337 GCGC G^CGC
HinfI 776 GAGTC G^A[ACGT]TC
MaeI 994 CTAG C^TAG
MluNI 744 TGGCCA TGG^CCA
MscI 744 TGGCCA TGG^CCA
NdeI 91 CATATG CA^TATG
NsiI 94 ATGCAT ATGCA^T
PaeR7I 556 CTCGAG C^TCGAG
PleI 776 GAGTC GAGTC(4/5)
SapI 315 GAAGAGC GCTCTTC(1/4)
Sau96I 626 GGCCC G^G[ACGT]CC
SfcI 909 CTGTAG C^T[GA][CT]AG
StyI 993 CCTAGG C^C[AT][AT]GG
XhoI 556 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AlwI 273 GATCC GGATC(4/5)
272 GGATC
BanI 724 GGTGCC G^G[CT][GA]CC
851 GGCACC
BsaJI 64 CCTCGG C^C[ACGT][ACGT]GG
993 CCTAGG
BssKI 807 CCTGG ^CC[ACGT]GG
855 CCAGG
BstNI 807 CCTGG CC^[AT]GG
855 CCAGG
EcoRII 807 CCTGG ^CC[AT]GG
855 CCAGG
HaeIII 626 GGCC GG^CC
745 GGCC
MvaI 807 CCTGG CC^[AT]GG
855 CCAGG
NspI 360 ACATGC [GA]CATG^[CT]
382 ACATGT
ScrFI 807 CCTGG CC^[ACGT]GG
855 CCAGG
SfaNI 327 GCATC GCATC(5/9)
516 GCATC
SmlI 556 CTCGAG C^T[CT][GA]AG
1162 CTTGAG
TaqI 519 TCGA T^CGA
557 TCGA
Tsp45I 386 GTGAC ^GT[GC]AC
479 GTGAC
TthHB8I 519 TCGA T^CGA
557 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AcsI AfaI AluI ApoI BglII
BsiYI BslI Bsp1407I BsrGI BsrI
BstYI DdeI DpnII EarI HindIII
HphI Ksp632I MaeIII MboI MboII
MflI MnlI MseI MslI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
SspBI Tru9I Tsp509I TspRI XhoII