################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BbrPI BbsI BbvCI BcgI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaI BseAI BsgI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV FokI FseI FspI HindIII HpaI KpnI KspI MfeI MluI MluNI MroI MscI MspA1I MunI NdeI NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 103 GTCTAC GT^[AC][GT]AC AccII 85 CGCG CG^CG AcsI 1069 GAATTC [GA]^AATT[CT] AcyI 1182 GGCGCC G[GA]^CG[CT]C AgeI 1499 ACCGGT A^CCGGT ApoI 1069 GAATTC [GA]^AATT[CT] Asp700I 246 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbeI 1182 GGCGCC GGCGC^C BciVI 413 GTATCC GTATCC(6/5) BclI 747 TGATCA T^GATCA BcnI 1241 CCGGG CC^[GC]GG BglII 373 AGATCT A^GATCT BsaBI 565 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1182 GGCGCC G[GA]^CG[CT]C BseRI 545 CTCCTC GAGGAG(10/8) BsmBI 1457 CGTCTC CGTCTC(1/5) BspHI 744 TCATGA T^CATGA BspMI 1225 GCAGGT ACCTGC(4/8) BsrDI 239 CATTGC GCAATG(2/0) BstAPI 981 GCAAGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 85 CGCG CG^CG EarI 1449 CTCTTC CTCTTC(1/4) EcoNI 1290 CCTGAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1069 GAATTC G^AATTC EcoT14I 950 CCATGG C^C[AT][AT]GG EcoT22I 146 ATGCAT ATGCA^T FbaI 747 TGATCA T^GATCA HaeII 1182 GGCGCC [GA]GCGC^[CT] HgaI 83 GACGC GACGC(5/10) Hin1I 1182 GGCGCC G[GA]^CG[CT]C HincII 681 GTTGAC GT[CT]^[GA]AC HindII 681 GTTGAC GT[CT]^[GA]AC KasI 1182 GGCGCC G^GCGCC Ksp632I 1449 CTCTTC CTCTTC(1/4) MamI 565 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 223 CACCCTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 85 CGCG CG^CG NaeI 1039 GCCGGC GCC^GGC NarI 1182 GGCGCC GG^CGCC NciI 1241 CCGGG CC^[GC]GG NcoI 950 CCATGG C^CATGG NgoMIV 1039 GCCGGC G^CCGGC NsiI 146 ATGCAT ATGCA^T PinAI 1499 ACCGGT A^CCGGT RcaI 744 TCATGA T^CATGA SacI 1467 GAGCTC GAGCT^C SexAI 1295 ACCAGGT A^CC[AT]GGT SfoI 1182 GGCGCC GGC^GCC StyI 950 CCATGG C^C[AT][AT]GG XmnI 246 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AspHI 1434 GAGCAC G[AT]GC[AT]^C 1467 GAGCTC BanII 599 GGGCTC G[GA]GC[CT]^C 1467 GAGCTC BsaWI 1255 ACCGGA [AT]^CCGG[AT] 1499 ACCGGT BsiHKAI 1434 GAGCAC G[AT]GC[AT]^C 1467 GAGCTC BsmAI 449 GAGAC GTCTC(1/5) 1458 GTCTC BsrBI 34 GAGCGG CCGCTC(-3/-3) 40 GAGCGG BsrFI 1039 GCCGGC [GA]^CCGG[CT] 1499 ACCGGT CfoI 1183 GCGC GCG^C 1337 GCGC Cfr10I 1039 GCCGGC [GA]^CCGG[CT] 1499 ACCGGT DsaI 950 CCATGG C^C[GA][CT]GG 1318 CCACGG FauI 36 GCGGG CCCGC(4/6) 42 GCGGG HhaI 1183 GCGC GCG^C 1337 GCGC HinP1I 1183 GCGC G^CGC 1337 GCGC MaeII 101 ACGT A^CGT 859 ACGT PleI 1282 GACTC GAGTC(4/5) 503 GAGTC PpuMI 486 AGGTCCT [GA]G^G[AT]CC[CT] 1010 AGGACCC PstI 785 CTGCAG CTGCA^G 988 CTGCAG SfcI 785 CTGCAG C^T[GA][CT]AG 988 CTGCAG TspRI 264 CAGTG CA[GC]TG[ACGT][ACGT]^ 703 CACTG XcmI 399 CCAGATTCTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1431 CCAGAGCACACTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BamHI BanI BbvI BfaI BmyI BpmI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TfiI Tru9I TseI Tsp45I Tsp509I XhoII