Restriction map for pF1KSDA1028
##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BalI        BbrPI       BbsI        
BbvCI       BcgI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaI        
BseAI       BsgI        BsiEI       BsiWI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspLU11I    
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstF5I      BstPI       BstXI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DraIII      DrdI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRV       FokI        FseI        FspI        HindIII     
HpaI        KpnI        KspI        MfeI        MluI        
MluNI       MroI        MscI        MspA1I      MunI        
NdeI        NheI        NotI        NruI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        PvuII       RsrII       SacII       
SalI        SapI        SbfI        ScaI        SfiI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TaqI        Tth111I     TthHB8I     
Van91I      XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccI          103  GTCTAC               GT^[AC][GT]AC
AccII          85  CGCG                 CG^CG
AcsI         1069  GAATTC               [GA]^AATT[CT]
AcyI         1182  GGCGCC               G[GA]^CG[CT]C
AgeI         1499  ACCGGT               A^CCGGT
ApoI         1069  GAATTC               [GA]^AATT[CT]
Asp700I       246  GAAGGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI         1182  GGCGCC               GGCGC^C
BciVI         413  GTATCC               GTATCC(6/5)
BclI          747  TGATCA               T^GATCA
BcnI         1241  CCGGG                CC^[GC]GG
BglII         373  AGATCT               A^GATCT
BsaBI         565  GATTTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        1182  GGCGCC               G[GA]^CG[CT]C
BseRI         545  CTCCTC               GAGGAG(10/8)
BsmBI        1457  CGTCTC               CGTCTC(1/5)
BspHI         744  TCATGA               T^CATGA
BspMI        1225  GCAGGT               ACCTGC(4/8)
BsrDI         239  CATTGC               GCAATG(2/0)
BstAPI        981  GCAAGCTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI          85  CGCG                 CG^CG
EarI         1449  CTCTTC               CTCTTC(1/4)
EcoNI        1290  CCTGAACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1069  GAATTC               G^AATTC
EcoT14I       950  CCATGG               C^C[AT][AT]GG
EcoT22I       146  ATGCAT               ATGCA^T
FbaI          747  TGATCA               T^GATCA
HaeII        1182  GGCGCC               [GA]GCGC^[CT]
HgaI           83  GACGC                GACGC(5/10)
Hin1I        1182  GGCGCC               G[GA]^CG[CT]C
HincII        681  GTTGAC               GT[CT]^[GA]AC
HindII        681  GTTGAC               GT[CT]^[GA]AC
KasI         1182  GGCGCC               G^GCGCC
Ksp632I      1449  CTCTTC               CTCTTC(1/4)
MamI          565  GATTTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI          223  CACCCTAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI           85  CGCG                 CG^CG
NaeI         1039  GCCGGC               GCC^GGC
NarI         1182  GGCGCC               GG^CGCC
NciI         1241  CCGGG                CC^[GC]GG
NcoI          950  CCATGG               C^CATGG
NgoMIV       1039  GCCGGC               G^CCGGC
NsiI          146  ATGCAT               ATGCA^T
PinAI        1499  ACCGGT               A^CCGGT
RcaI          744  TCATGA               T^CATGA
SacI         1467  GAGCTC               GAGCT^C
SexAI        1295  ACCAGGT              A^CC[AT]GGT
SfoI         1182  GGCGCC               GGC^GCC
StyI          950  CCATGG               C^C[AT][AT]GG
XmnI          246  GAAGGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AspHI        1434  GAGCAC               G[AT]GC[AT]^C
             1467  GAGCTC              
BanII         599  GGGCTC               G[GA]GC[CT]^C
             1467  GAGCTC              
BsaWI        1255  ACCGGA               [AT]^CCGG[AT]
             1499  ACCGGT              
BsiHKAI      1434  GAGCAC               G[AT]GC[AT]^C
             1467  GAGCTC              
BsmAI         449  GAGAC                GTCTC(1/5)
             1458  GTCTC               
BsrBI          34  GAGCGG               CCGCTC(-3/-3)
               40  GAGCGG              
BsrFI        1039  GCCGGC               [GA]^CCGG[CT]
             1499  ACCGGT              
CfoI         1183  GCGC                 GCG^C
             1337  GCGC                
Cfr10I       1039  GCCGGC               [GA]^CCGG[CT]
             1499  ACCGGT              
DsaI          950  CCATGG               C^C[GA][CT]GG
             1318  CCACGG              
FauI           36  GCGGG                CCCGC(4/6)
               42  GCGGG               
HhaI         1183  GCGC                 GCG^C
             1337  GCGC                
HinP1I       1183  GCGC                 G^CGC
             1337  GCGC                
MaeII         101  ACGT                 A^CGT
              859  ACGT                
PleI         1282  GACTC                GAGTC(4/5)
              503  GAGTC               
PpuMI         486  AGGTCCT              [GA]G^G[AT]CC[CT]
             1010  AGGACCC             
PstI          785  CTGCAG               CTGCA^G
              988  CTGCAG              
SfcI          785  CTGCAG               C^T[GA][CT]AG
              988  CTGCAG              
TspRI         264  CAGTG                CA[GC]TG[ACGT][ACGT]^
              703  CACTG               
XcmI          399  CCAGATTCTTGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1431  CCAGAGCACACTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BamHI       BanI        BbvI        BfaI        BmyI        
BpmI        BsaJI       BsiYI       BslI        BsmFI       
Bsp1286I    BsrI        BssKI       BstNI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       DraII       
EcoO109I    EcoRII      Fnu4HI      HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        MaeI        
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     XhoII       
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