################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco47III EcoO65I EcoRV EcoT22I FbaI FokI FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I MaeI MaeII MaeIII MamI MfeI MluI MroI MseI MslI MunI NaeI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp45I Tth111I Van91I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AcsI 534 GAATTC [GA]^AATT[CT] AfaI 420 GTAC GT^AC AlwI 615 GGATC GGATC(4/5) AlwNI 418 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 440 GGGCCC GGGCC^C ApoI 534 GAATTC [GA]^AATT[CT] AspHI 259 GAGCAC G[AT]GC[AT]^C BalI 153 TGGCCA TGG^CCA BanI 323 GGCGCC G^G[CT][GA]CC BanII 440 GGGCCC G[GA]GC[CT]^C BbeI 323 GGCGCC GGCGC^C BbsI 49 GTCTTC GAAGAC(2/6) BglI 442 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 485 AGATCT A^GATCT BpuAI 49 GTCTTC GAAGAC(2/6) BseRI 687 CTCCTC GAGGAG(10/8) BsiEI 27 CGGCCG CG[GA][CT]^CG BsiHKAI 259 GAGCAC G[AT]GC[AT]^C BspMI 252 ACCTGC ACCTGC(4/8) BsrBI 319 GAGCGG CCGCTC(-3/-3) BsrI 644 CCAGT ACTGG(1/-1) EagI 27 CGGCCG C^GGCCG EclXI 27 CGGCCG C^GGCCG Eco52I 27 CGGCCG C^GGCCG EcoNI 137 CCTACTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 534 GAATTC G^AATTC EcoT14I 623 CCAAGG C^C[AT][AT]GG HgaI 34 GACGC GACGC(5/10) HphI 467 TCACC GGTGA(8/7) KasI 323 GGCGCC G^GCGCC MboII 50 TCTTC GAAGA(8/7) MluNI 153 TGGCCA TGG^CCA MscI 153 TGGCCA TGG^CCA NarI 323 GGCGCC GG^CGCC PaeR7I 274 CTCGAG C^TCGAG RsaI 420 GTAC GT^AC SfaNI 681 GCATC GCATC(5/9) SfoI 323 GGCGCC GGC^GCC SmlI 274 CTCGAG C^T[CT][GA]AG StyI 623 CCAAGG C^C[AT][AT]GG TaqI 275 TCGA T^CGA TspRI 645 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 275 TCGA T^CGA XhoI 274 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccII 225 CGCG CG^CG 296 CGCG AcyI 34 GACGCC G[GA]^CG[CT]C 323 GGCGCC AocI 265 CCTCAGG CC^T[ACGT]AGG 328 CCTCAGG AvaI 274 CTCGAG C^[CT]CG[GA]G 433 CCCGAG BmyI 259 GAGCAC G[AGT]GC[ACT]^C 440 GGGCCC BpmI 141 CTCCAG CTGGAG(16/14) 372 CTCCAG BsaHI 34 GACGCC G[GA]^CG[CT]C 323 GGCGCC BsmAI 197 GAGAC GTCTC(1/5) 582 GTCTC BsoBI 274 CTCGAG C^[CT]CG[GA]G 433 CCCGAG Bsp1286I 259 GAGCAC G[AGT]GC[ACT]^C 440 GGGCCC BstUI 225 CGCG CG^CG 296 CGCG BstYI 485 AGATCT [GA]^GATC[CT] 615 GGATCT Bsu36I 265 CCTCAGG CC^T[ACGT]AGG 328 CCTCAGG DpnII 486 GATC ^GATC 616 GATC DsaI 224 CCGCGG C^C[GA][CT]GG 295 CCGCGG EaeI 27 CGGCCG [CT]^GGCC[GA] 153 TGGCCA Eco81I 265 CCTCAGG CC^T[ACGT]AGG 328 CCTCAGG Hin1I 34 GACGCC G[GA]^CG[CT]C 323 GGCGCC HinfI 199 GACTC G^A[ACGT]TC 283 GACTC KspI 224 CCGCGG CCGC^GG 295 CCGCGG MboI 486 GATC ^GATC 616 GATC MflI 485 AGATCT [GA]^GATC[CT] 615 GGATCT MvnI 225 CGCG CG^CG 296 CGCG NdeII 486 GATC ^GATC 616 GATC PleI 199 GACTC GAGTC(4/5) 283 GACTC PpuMI 568 AGGACCT [GA]G^G[AT]CC[CT] 631 GGGACCC PvuII 382 CAGCTG CAG^CTG 445 CAGCTG SacII 224 CCGCGG CCGC^GG 295 CCGCGG Sau3AI 486 GATC ^GATC 616 GATC Tsp509I 130 AATT ^AATT 535 AATT XhoII 485 AGATCT [GA]^GATC[CT] 615 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI AluI AvaII BbvI BcnI BsaJI BsiYI BslI BsmFI BssKI BstNI Cac8I CfoI Cfr13I DdeI DraII EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI MnlI MspA1I MspI MvaI MwoI NciI NlaIV PstI Sau96I ScrFI SfcI TseI