Restriction map for
pF1KB7864
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# NO CUT ENZYMES ( 114 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BamHI BbeI BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BsaBI BsaHI BsaWI BseAI BsiWI
BspDI BspEI BspLU11I BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MseI MunI
MvnI NarI NdeI NheI NotI
NruI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI TfiI Tru9I
Tsp509I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AflIII 851 ACACGT A^C[GA][CT]GT
AlwI 636 GATCC GGATC(4/5)
AocI 280 CCTGAGG CC^T[ACGT]AGG
AspI 1026 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 1288 GGTGCC G^G[CT][GA]CC
BbvCI 273 CCTCAGC CCTCAGC(-5/-2)
BsaAI 852 CACGTA [CT]AC^GT[GA]
BsiEI 156 CGGTCG CG[GA][CT]^CG
BsmI 718 GAATGC GAATGC(1/-1)
Bsp1407I 485 TGTACA T^GTACA
BspHI 1232 TCATGA T^CATGA
BsrDI 1811 GCAATG GCAATG(2/0)
BsrGI 485 TGTACA T^GTACA
BstAPI 1557 GCAGACTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 635 AGATCC [GA]^GATC[CT]
Bsu36I 280 CCTGAGG CC^T[ACGT]AGG
DpnII 636 GATC ^GATC
Eco81I 280 CCTGAGG CC^T[ACGT]AGG
EcoT22I 720 ATGCAT ATGCA^T
MboI 636 GATC ^GATC
MflI 635 AGATCC [GA]^GATC[CT]
NdeII 636 GATC ^GATC
NsiI 720 ATGCAT ATGCA^T
PflFI 1026 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 81 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1232 TCATGA T^CATGA
SapI 559 GAAGAGC GCTCTTC(1/4)
Sau3AI 636 GATC ^GATC
SexAI 490 ACCTGGT A^CC[AT]GGT
SmaI 882 CCCGGG CCC^GGG
SmlI 1002 CTCAAG C^T[CT][GA]AG
SspBI 485 TGTACA T^GTACA
Tth111I 1026 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 81 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 635 AGATCC [GA]^GATC[CT]
XmaCI 882 CCCGGG C^CCGGG
XmaI 882 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 790 GTCTAC GT^[AC][GT]AC
1032 GTCTAC
Asp700I 603 GAACCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1458 GAACCCCTTC
AspHI 31 GAGCTC G[AT]GC[AT]^C
1797 GAGCTC
BfaI 671 CTAG C^TAG
694 CTAG
BglI 180 GCCCAATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1471 GCCACCATGGC
BsiHKAI 31 GAGCTC G[AT]GC[AT]^C
1797 GAGCTC
BsmBI 217 GAGACG CGTCTC(1/5)
688 GAGACG
BsrBI 899 CCGCTC CCGCTC(-3/-3)
1423 CCGCTC
EarI 1881 CTCTTC CTCTTC(1/4)
559 GAAGAG
EcoT14I 845 CCATGG C^C[AT][AT]GG
1475 CCATGG
HaeII 1591 AGCGCC [GA]GCGC^[CT]
1753 AGCGCC
Ksp632I 1881 CTCTTC CTCTTC(1/4)
559 GAAGAG
MaeI 671 CTAG C^TAG
694 CTAG
NcoI 845 CCATGG C^CATGG
1475 CCATGG
SacI 31 GAGCTC GAGCT^C
1797 GAGCTC
StyI 845 CCATGG C^C[AT][AT]GG
1475 CCATGG
XmnI 603 GAACCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1458 GAACCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AfaI AluI AlwNI ApaI
AvaI AvaII BalI BanII BbsI
BbvI BcnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstXI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DraII DraIII DsaI EaeI EcoNI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NaeI NciI NgoMIV NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau96I ScrFI SfaNI SfcI StuI
TaqI TseI Tsp45I TspRI TthHB8I