Restriction map for
pF1KB7874
##################################
# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
AlwI AocI Aor51HI ApaI ApoI
AscI AseI AsnI AspEI AvaI
AviII AvrII BamHI BbeI BbrPI
BcgI BclI BcnI BfrI BglI
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaI BsaWI BseAI BsiWI BsmAI
BsmBI BsoBI BspDI BspEI BspHI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeII
HapII Hin1I HpaI HpaII KasI
Ksp632I KspI MamI MluI MroI
MspI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SpeI Sse8387I StuI StyI SwaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Alw44I 1033 GTGCAC G^TGCAC
ApaLI 1033 GTGCAC G^TGCAC
Asp700I 1381 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1033 GTGCAC G[AT]GC[AT]^C
AspI 1475 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 1327 GAGCCC G[GA]GC[CT]^C
BbsI 1266 GAAGAC GAAGAC(2/6)
BciVI 1133 GGATAC GTATCC(6/5)
BpmI 50 CTGGAG CTGGAG(16/14)
BpuAI 1266 GAAGAC GAAGAC(2/6)
BsaAI 1407 TACGTA [CT]AC^GT[GA]
BsiEI 999 CGGTCG CG[GA][CT]^CG
BsiHKAI 1033 GTGCAC G[AT]GC[AT]^C
BsmI 1711 GCATTC GAATGC(1/-1)
BstBI 988 TTCGAA TT^CGAA
BstXI 1562 CCATCACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 67 GCGC GCG^C
DsaI 104 CCGTGG C^C[GA][CT]GG
EcoNI 49 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HhaI 67 GCGC GCG^C
HinP1I 67 GCGC G^CGC
HindIII 144 AAGCTT A^AGCTT
MspA1I 403 CAGCTG C[AC]G^C[GT]G
NdeI 1154 CATATG CA^TATG
NspV 988 TTCGAA TT^CGAA
PflFI 1475 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1081 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1396 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 403 CAGCTG CAG^CTG
SfcI 937 CTACAG C^T[GA][CT]AG
SfuI 988 TTCGAA TT^CGAA
SnaBI 1407 TACGTA TAC^GTA
SphI 807 GCATGC GCATG^C
SspI 1196 AATATT AAT^ATT
Tth111I 1475 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1081 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 536 CCATCAGTGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1381 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 337 GGTACC G^GTACC
415 GGTACC
AflIII 72 ACATGT A^C[GA][CT]GT
267 ACATGT
AlwNI 596 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG
1166 CAGTTCCTG
Asp718I 337 GGTACC G^GTACC
415 GGTACC
BalI 222 TGGCCA TGG^CCA
1292 TGGCCA
BbvCI 1625 CCTCAGC CCTCAGC(-5/-2)
1660 GCTGAGG
BfaI 304 CTAG C^TAG
1874 CTAG
BglII 933 AGATCT A^GATCT
1280 AGATCT
BsgI 502 CTGCAC GTGCAG(16/14)
1071 CTGCAC
Bsp1407I 1162 TGTACA T^GTACA
1208 TGTACA
BspLU11I 72 ACATGT A^CATGT
267 ACATGT
BspMI 496 GCAGGT ACCTGC(4/8)
595 GCAGGT
BsrGI 1162 TGTACA T^GTACA
1208 TGTACA
BstYI 933 AGATCT [GA]^GATC[CT]
1280 AGATCT
DraII 970 AGGCCCT [GA]G^G[ACGT]CC[CT]
1396 GGGTCCC
EaeI 222 TGGCCA [CT]^GGCC[GA]
1292 TGGCCA
EcoO109I 970 AGGCCCT [GA]G^G[ACGT]CC[CT]
1396 GGGTCCC
FauI 450 CCCGC CCCGC(4/6)
1603 CCCGC
HgaI 300 GCGTC GACGC(5/10)
1870 GCGTC
HincII 1481 GTCAAC GT[CT]^[GA]AC
1522 GTCAAC
HindII 1481 GTCAAC GT[CT]^[GA]AC
1522 GTCAAC
KpnI 337 GGTACC GGTAC^C
415 GGTACC
MaeI 304 CTAG C^TAG
1874 CTAG
MfeI 521 CAATTG C^AATTG
1688 CAATTG
MflI 933 AGATCT [GA]^GATC[CT]
1280 AGATCT
MluNI 222 TGGCCA TGG^CCA
1292 TGGCCA
MscI 222 TGGCCA TGG^CCA
1292 TGGCCA
MunI 521 CAATTG C^AATTG
1688 CAATTG
PleI 1512 GACTC GAGTC(4/5)
1479 GAGTC
SspBI 1162 TGTACA T^GTACA
1208 TGTACA
TfiI 25 GATTC G^A[AT]TC
1877 GAATC
XbaI 303 TCTAGA T^CTAGA
1873 TCTAGA
XhoII 933 AGATCT [GA]^GATC[CT]
1280 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI AvaII BanI
BbvI BmyI BsaJI BseRI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeII MaeIII
MboI MboII MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NspI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I