Restriction map for
pF1KA0008
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI Alw44I
AlwI Aor51HI ApaI ApaLI AscI
Asp718I AspHI AvaI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbvCI BcgI BciVI BcnI BfrI
BglI BlnI BmyI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsoBI Bsp1286I
BspDI BspEI BspHI BsrBI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstXI BstZ17I Cac8I CfoI ClaI
CpoI DraIII DrdI EaeI EagI
EclXI Eco47III Eco52I EcoRV EcoT22I
FauI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI RsrII SacI SacII SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 23 GTCGAC GT^[AC][GT]AC
AhdI 1874 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 925 CCTAAGG CC^T[ACGT]AGG
AseI 2209 ATTAAT AT^TAAT
AsnI 2209 ATTAAT AT^TAAT
Asp700I 912 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1874 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 527 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 723 GGTGCC G^G[CT][GA]CC
BseRI 2457 GAGGAG GAGGAG(10/8)
BsgI 583 GTGCAG GTGCAG(16/14)
Bsp1407I 1507 TGTACA T^GTACA
BsrFI 1996 GCCGGT [GA]^CCGG[CT]
BsrGI 1507 TGTACA T^GTACA
BstEII 2497 GGTAACC G^GT[ACGT]ACC
BstPI 2497 GGTAACC G^GT[ACGT]ACC
Bsu36I 925 CCTAAGG CC^T[ACGT]AGG
Cfr10I 1996 GCCGGT [GA]^CCGG[CT]
DsaI 1281 CCATGG C^C[GA][CT]GG
Eam1105I 1874 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 925 CCTAAGG CC^T[ACGT]AGG
EcoNI 1428 CCTTATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2497 GGTAACC G^GT[ACGT]ACC
HapII 1997 CCGG C^CGG
HpaII 1997 CCGG C^CGG
MaeII 601 ACGT A^CGT
MspA1I 2449 CAGCTG C[AC]G^C[GT]G
MspI 1997 CCGG C^CGG
NcoI 1281 CCATGG C^CATGG
PflFI 527 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 946 CCACTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1149 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 2209 ATTAAT AT^TAAT
PstI 2430 CTGCAG CTGCA^G
PvuII 2449 CAGCTG CAG^CTG
SalI 23 GTCGAC G^TCGAC
ScaI 43 AGTACT AGT^ACT
SfcI 2430 CTGCAG C^T[GA][CT]AG
SspBI 1507 TGTACA T^GTACA
SspI 348 AATATT AAT^ATT
SwaI 1211 ATTTAAAT ATTT^AAAT
Tsp45I 685 GTCAC ^GT[GC]AC
Tth111I 527 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 946 CCACTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 470 CCAAAGACCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 912 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 1176 ACATGT A^C[GA][CT]GT
2022 ACATGT
BclI 261 TGATCA T^GATCA
1580 TGATCA
BfaI 256 CTAG C^TAG
1726 CTAG
BglII 1392 AGATCT A^GATCT
1512 AGATCT
BlpI 418 GCTGAGC GC^T[ACGT]AGC
1270 GCTCAGC
BpmI 1309 CTCCAG CTGGAG(16/14)
2452 CTGGAG
Bpu1102I 418 GCTGAGC GC^T[ACGT]AGC
1270 GCTCAGC
BsmFI 1352 GGGAC GGGAC(10/14)
1237 GTCCC
BspLU11I 1176 ACATGT A^CATGT
2022 ACATGT
BspMI 741 ACCTGC ACCTGC(4/8)
642 GCAGGT
BstYI 1392 AGATCT [GA]^GATC[CT]
1512 AGATCT
CelII 418 GCTGAGC GC^T[ACGT]AGC
1270 GCTCAGC
DraII 1149 GGGTCCT [GA]G^G[ACGT]CC[CT]
1893 AGGCCCT
EarI 1884 CTCTTC CTCTTC(1/4)
2180 CTCTTC
EcoO109I 1149 GGGTCCT [GA]G^G[ACGT]CC[CT]
1893 AGGCCCT
FbaI 261 TGATCA T^GATCA
1580 TGATCA
HincII 23 GTCGAC GT[CT]^[GA]AC
1927 GTCAAC
HindII 23 GTCGAC GT[CT]^[GA]AC
1927 GTCAAC
Ksp632I 1884 CTCTTC CTCTTC(1/4)
2180 CTCTTC
MaeI 256 CTAG C^TAG
1726 CTAG
MflI 1392 AGATCT [GA]^GATC[CT]
1512 AGATCT
MslI 1082 CACAAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1279 CACCATGGTG
SexAI 528 ACCTGGT A^CC[AT]GGT
2418 ACCAGGT
XhoII 1392 AGATCT [GA]^GATC[CT]
1512 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AcsI AfaI AluI AlwNI ApoI
AvaII BbsI BbvI BpuAI BsaJI
BsiYI BslI BsmAI BsmI BsrDI
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DpnII DraI EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeIII MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I