Restriction map for
pF1KA0011
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# NO CUT ENZYMES ( 101 enzymes) #
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Acc65I AccII AclI AflII AflIII
AgeI AhdI Alw44I ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI BalI BbeI BbrPI
BbvCI BfrI BsaAI BsaBI BsiEI
BsiWI BsmBI BsoBI BspDI BspHI
BspLU11I BssHII Bst1107I BstAPI BstUI
BstZ17I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
EcoRI EcoRV EcoT22I HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MscI MunI MvnI
NarI NcoI NdeI NheI NotI
NruI NsiI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 1931 GACGTC GACGT^C
AccIII 1545 TCCGGA T^CCGGA
AcsI 1915 AAATTC [GA]^AATT[CT]
AcyI 1931 GACGTC G[GA]^CG[CT]C
AlwNI 586 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 1915 AAATTC [GA]^AATT[CT]
AviII 2447 TGCGCA TGC^GCA
AvrII 280 CCTAGG C^CTAGG
BamHI 2137 GGATCC G^GATCC
BbsI 736 GAAGAC GAAGAC(2/6)
BcgI 1225 GCAGGAGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 192 TGATCA T^GATCA
BlnI 280 CCTAGG C^CTAGG
BlpI 1561 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1561 GCTCAGC GC^T[ACGT]AGC
BpuAI 736 GAAGAC GAAGAC(2/6)
BsaHI 1931 GACGTC G[GA]^CG[CT]C
BseAI 1545 TCCGGA T^CCGGA
BsgI 131 GTGCAG GTGCAG(16/14)
BsmI 2469 GCATTC GAATGC(1/-1)
Bsp1407I 1077 TGTACA T^GTACA
BspEI 1545 TCCGGA T^CCGGA
BsrDI 2187 CATTGC GCAATG(2/0)
BsrFI 40 GCCGGC [GA]^CCGG[CT]
BsrGI 1077 TGTACA T^GTACA
BssSI 836 CACGAG CACGAG(-5/-1)
BstBI 2582 TTCGAA TT^CGAA
BstEII 2074 GGTTACC G^GT[ACGT]ACC
BstPI 2074 GGTTACC G^GT[ACGT]ACC
BstXI 1150 CCAGAGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1561 GCTCAGC GC^T[ACGT]AGC
Cfr10I 40 GCCGGC [GA]^CCGG[CT]
EcoO65I 2074 GGTTACC G^GT[ACGT]ACC
FbaI 192 TGATCA T^GATCA
FseI 39 GGCCGGCC GGCCGG^CC
FspI 2447 TGCGCA TGC^GCA
HgaI 2068 GACGC GACGC(5/10)
Hin1I 1931 GACGTC G[GA]^CG[CT]C
HincII 2371 GTCAAC GT[CT]^[GA]AC
HindII 2371 GTCAAC GT[CT]^[GA]AC
MroI 1545 TCCGGA T^CCGGA
MseI 1801 TTAA T^TAA
NaeI 40 GCCGGC GCC^GGC
NgoMIV 40 GCCGGC G^CCGGC
NspI 2450 GCATGC [GA]CATG^[CT]
NspV 2582 TTCGAA TT^CGAA
PvuII 2497 CAGCTG CAG^CTG
SapI 598 GAAGAGC GCTCTTC(1/4)
ScaI 1521 AGTACT AGT^ACT
SfuI 2582 TTCGAA TT^CGAA
SphI 2450 GCATGC GCATG^C
SspBI 1077 TGTACA T^GTACA
StuI 2090 AGGCCT AGG^CCT
Tru9I 1801 TTAA T^TAA
Tsp509I 1916 AATT ^AATT
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# TWO CUT ENZYMES ( 16 enzymes) #
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AccI 67 GTAGAC GT^[AC][GT]AC
1535 GTCTAC
AocI 514 CCTGAGG CC^T[ACGT]AGG
637 CCTGAGG
Aor51HI 1154 AGCGCT AGC^GCT
1961 AGCGCT
BciVI 3 GGATAC GTATCC(6/5)
1778 GGATAC
BcnI 1481 CCCGG CC^[GC]GG
2177 CCGGG
BglII 246 AGATCT A^GATCT
2271 AGATCT
BsaWI 1545 TCCGGA [AT]^CCGG[AT]
1904 ACCGGA
BsrBI 1034 CCGCTC CCGCTC(-3/-3)
1186 CCGCTC
Bsu36I 514 CCTGAGG CC^T[ACGT]AGG
637 CCTGAGG
EaeI 297 TGGCCG [CT]^GGCC[GA]
484 CGGCCA
Eco47III 1154 AGCGCT AGC^GCT
1961 AGCGCT
Eco81I 514 CCTGAGG CC^T[ACGT]AGG
637 CCTGAGG
HaeII 1154 AGCGCT [GA]GCGC^[CT]
1961 AGCGCT
MspA1I 1774 CAGCGG C[AC]G^C[GT]G
2497 CAGCTG
NciI 1481 CCCGG CC^[GC]GG
2177 CCGGG
XcmI 1371 CCACTTTGTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2589 CCATTTTGTCCGTGG
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# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AfaI AluI AlwI ApaI
AspHI AvaII BanI BanII BbvI
BfaI BglI BmyI BpmI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV PleI PpuMI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I
XhoII