Restriction map for
pF1KA0030
##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
Alw44I AlwNI ApaLI AscI AseI
AsnI Asp718I AvaI BamHI BbeI
BfrI BsaWI BseAI BsiWI BsmI
BsoBI BspDI BspEI BssSI Bst1107I
BstBI BstZ17I ClaI CpoI DraI
DrdI EcoRI EcoT22I HindIII HpaI
KasI KpnI KspI MfeI MluI
MroI MseI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII ScaI SexAI SfiI SfoI
SfuI SmaI SnaBI SpeI SspI
SwaI Tru9I Van91I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 2368 GACGTC GACGT^C
AccI 619 GTCGAC GT^[AC][GT]AC
AcsI 1762 GAATTT [GA]^AATT[CT]
AhdI 315 GACGGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2098 AGCGCT AGC^GCT
ApoI 1762 GAATTT [GA]^AATT[CT]
Asp700I 2664 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 315 GACGGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 168 CCTAGG C^CTAGG
BbvCI 888 GCTGAGG CCTCAGC(-5/-2)
BcgI 611 GCACTCACGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1124 GTATCC GTATCC(6/5)
BclI 1388 TGATCA T^GATCA
BglI 265 GCCTATGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 168 CCTAGG C^CTAGG
BsaBI 2613 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1226 GAGACG CGTCTC(1/5)
Bsp1407I 2237 TGTACA T^GTACA
BspHI 1461 TCATGA T^CATGA
BspLU11I 662 ACATGT A^CATGT
BsrGI 2237 TGTACA T^GTACA
BssHII 2332 GCGCGC G^CGCGC
BstAPI 986 GCAACAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 933 GGTGACC G^GT[ACGT]ACC
BstPI 933 GGTGACC G^GT[ACGT]ACC
DraIII 538 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1070 CGGCCG C^GGCCG
Eam1105I 315 GACGGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1070 CGGCCG C^GGCCG
Eco47III 2098 AGCGCT AGC^GCT
Eco52I 1070 CGGCCG C^GGCCG
EcoO65I 933 GGTGACC G^GT[ACGT]ACC
EcoRV 1546 GATATC GAT^ATC
FbaI 1388 TGATCA T^GATCA
FseI 1071 GGCCGGCC GGCCGG^CC
MamI 2613 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
RcaI 1461 TCATGA T^CATGA
SalI 619 GTCGAC G^TCGAC
SbfI 1857 CCTGCAGG CCTGCA^GG
SgrAI 752 CACCGGCG C[GA]^CCGG[CT]G
SmlI 1597 CTCAAG C^T[CT][GA]AG
Sse8387I 1857 CCTGCAGG CCTGCA^GG
SspBI 2237 TGTACA T^GTACA
StuI 1479 AGGCCT AGG^CCT
XmnI 2664 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflIII 662 ACATGT A^C[GA][CT]GT
2123 ACACGT
AocI 745 CCTGAGG CC^T[ACGT]AGG
2247 CCTGAGG
AspI 2002 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2365 GACGACGTC
BanI 750 GGCACC G^G[CT][GA]CC
1685 GGCACC
BbrPI 724 CACGTG CAC^GTG
1327 CACGTG
BlpI 963 GCTCAGC GC^T[ACGT]AGC
2104 GCTGAGC
Bpu1102I 963 GCTCAGC GC^T[ACGT]AGC
2104 GCTGAGC
BsaI 1099 GAGACC GGTCTC(1/5)
1568 GAGACC
BsgI 942 CTGCAC GTGCAG(16/14)
1869 CTGCAC
BsiEI 822 CGACCG CG[GA][CT]^CG
1070 CGGCCG
BspMI 860 ACCTGC ACCTGC(4/8)
2526 GCAGGT
BstXI 923 CCAGTGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1322 CCAACCACGTGG
Bsu36I 745 CCTGAGG CC^T[ACGT]AGG
2247 CCTGAGG
CelII 963 GCTCAGC GC^T[ACGT]AGC
2104 GCTGAGC
Eco81I 745 CCTGAGG CC^T[ACGT]AGG
2247 CCTGAGG
NaeI 1072 GCCGGC GCC^GGC
1163 GCCGGC
NcoI 26 CCATGG C^CATGG
1808 CCATGG
NgoMIV 1072 GCCGGC G^CCGGC
1163 GCCGGC
PflFI 2002 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2365 GACGACGTC
PmaCI 724 CACGTG CAC^GTG
1327 CACGTG
PmlI 724 CACGTG CAC^GTG
1327 CACGTG
SacI 211 GAGCTC GAGCT^C
2650 GAGCTC
SapI 2496 GCTCTTC GCTCTTC(1/4)
2652 GCTCTTC
SphI 389 GCATGC GCATG^C
2438 GCATGC
Tsp509I 997 AATT ^AATT
1763 AATT
Tth111I 2002 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2365 GACGACGTC
XcmI 248 CCATCCCAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2306 CCATGATCCGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AspHI AvaII AviII
BalI BanII BbsI BbvI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaAI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrBI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I XhoII