Restriction map for
pF1KA0078
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AvaI
AviII AvrII BbeI BbrPI BcgI
BciVI BcnI BfrI BglI BglII
BlnI BsaAI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmFI
BsoBI Bsp1407I BspEI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstUI BstXI
BstZ17I CfoI Cfr10I CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
FauI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HindIII
HpaI HpaII KasI KpnI KspI
MaeII MfeI MluI MroI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NotI
NruI PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AflIII 542 ACATGT A^C[GA][CT]GT
Asp700I 334 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 979 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 41 TGGCCA TGG^CCA
BamHI 843 GGATCC G^GATCC
BanI 1059 GGCACC G^G[CT][GA]CC
BbvCI 1471 CCTCAGC CCTCAGC(-5/-2)
BlpI 1129 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1129 GCTCAGC GC^T[ACGT]AGC
BsaBI 1642 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 1876 CCAAGG C^C[ACGT][ACGT]GG
BsgI 776 CTGCAC GTGCAG(16/14)
BsmAI 1093 GAGAC GTCTC(1/5)
BsmI 241 GCATTC GAATGC(1/-1)
BspHI 342 TCATGA T^CATGA
BspLU11I 542 ACATGT A^CATGT
BstBI 191 TTCGAA TT^CGAA
CelII 1129 GCTCAGC GC^T[ACGT]AGC
DraII 33 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 33 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1243 GAATTC G^AATTC
EcoT14I 1876 CCAAGG C^C[AT][AT]GG
EcoT22I 1628 ATGCAT ATGCA^T
MamI 1642 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 41 TGGCCA TGG^CCA
MscI 41 TGGCCA TGG^CCA
MslI 378 CATCGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 54 GCTAGC G^CTAGC
NsiI 1628 ATGCAT ATGCA^T
NspI 542 ACATGT [GA]CATG^[CT]
NspV 191 TTCGAA TT^CGAA
PflFI 979 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 44 CCAAAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 342 TCATGA T^CATGA
SapI 1336 GAAGAGC GCTCTTC(1/4)
SfuI 191 TTCGAA TT^CGAA
SmlI 1744 CTTGAG C^T[CT][GA]AG
SspI 681 AATATT AAT^ATT
StyI 1876 CCAAGG C^C[AT][AT]GG
Tsp45I 1853 GTGAC ^GT[GC]AC
Tth111I 979 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 44 CCAAAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 334 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AfaI 1189 GTAC GT^AC
1848 GTAC
AlwNI 355 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
1454 CAGAGCCTG
AocI 289 CCTGAGG CC^T[ACGT]AGG
1417 CCTCAGG
AspHI 591 GAGCAC G[AT]GC[AT]^C
1706 GTGCTC
AvaII 1284 GGACC G^G[AT]CC
1873 GGACC
BanII 1013 GAGCCC G[GA]GC[CT]^C
1324 GAGCCC
BbsI 1195 GAAGAC GAAGAC(2/6)
1697 GTCTTC
BclI 351 TGATCA T^GATCA
873 TGATCA
BfaI 55 CTAG C^TAG
1277 CTAG
BpmI 1351 CTCCAG CTGGAG(16/14)
1721 CTGGAG
BpuAI 1195 GAAGAC GAAGAC(2/6)
1697 GTCTTC
BsiHKAI 591 GAGCAC G[AT]GC[AT]^C
1706 GTGCTC
BspDI 379 ATCGAT AT^CGAT
1318 ATCGAT
BsrI 180 ACTGG ACTGG(1/-1)
1720 ACTGG
BstYI 843 GGATCC [GA]^GATC[CT]
1053 GGATCT
Bsu36I 289 CCTGAGG CC^T[ACGT]AGG
1417 CCTCAGG
ClaI 379 ATCGAT AT^CGAT
1318 ATCGAT
EaeI 41 TGGCCA [CT]^GGCC[GA]
268 CGGCCA
Eco81I 289 CCTGAGG CC^T[ACGT]AGG
1417 CCTCAGG
EcoNI 285 CCTGCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
741 CCTCTCTGAGG
FbaI 351 TGATCA T^GATCA
873 TGATCA
HincII 280 GTTGAC GT[CT]^[GA]AC
976 GTTGAC
HindII 280 GTTGAC GT[CT]^[GA]AC
976 GTTGAC
MaeI 55 CTAG C^TAG
1277 CTAG
MaeIII 1042 GTTAC ^GT[ACGT]AC
1853 GTGAC
MflI 843 GGATCC [GA]^GATC[CT]
1053 GGATCT
RsaI 1189 GTAC GT^AC
1848 GTAC
SfcI 1502 CTGTAG C^T[GA][CT]AG
1791 CTACAG
XhoII 843 GGATCC [GA]^GATC[CT]
1053 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BbvI BmyI BseRI BsiYI BslI
Bsp1286I BspMI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI SfaNI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I