Restriction map for
pF1KA0107
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BciVI BfrI
BglI BlnI BsaAI BsaHI BsaI
BsaWI BseAI BsgI BsiWI Bsp1407I
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstXI BstZ17I Cfr10I
CpoI DraI DraII DraIII DrdI
Eam1105I Eco47III EcoO109I EcoRV FauI
FseI FspI HaeII Hin1I HincII
HindII HpaI KasI KpnI MfeI
MluI MluNI MroI MscI MslI
MunI NaeI NarI NcoI NgoMIV
NheI NotI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI RcaI RsrII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AocI 432 CCTTAGG CC^T[ACGT]AGG
AseI 1159 ATTAAT AT^TAAT
AsnI 1159 ATTAAT AT^TAAT
AvaI 85 CCCGAG C^[CT]CG[GA]G
AvaII 201 GGACC G^G[AT]CC
BanII 356 GAGCTC G[GA]GC[CT]^C
BbsI 755 GTCTTC GAAGAC(2/6)
BcgI 173 GCAAATCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 990 TGATCA T^GATCA
BcnI 1019 CCGGG CC^[GC]GG
BfaI 283 CTAG C^TAG
BlpI 75 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 75 GCTCAGC GC^T[ACGT]AGC
BpuAI 755 GTCTTC GAAGAC(2/6)
BsaBI 457 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 128 CGGCCG CG[GA][CT]^CG
BsmBI 98 GAGACG CGTCTC(1/5)
BsmFI 521 GGGAC GGGAC(10/14)
BsmI 793 GAATGC GAATGC(1/-1)
BsoBI 85 CCCGAG C^[CT]CG[GA]G
BspDI 875 ATCGAT AT^CGAT
BsrDI 307 GCAATG GCAATG(2/0)
BstEII 403 GGTCACC G^GT[ACGT]ACC
BstPI 403 GGTCACC G^GT[ACGT]ACC
Bsu36I 432 CCTTAGG CC^T[ACGT]AGG
Cac8I 80 GCCTGC GC[ACGT]^[ACGT]GC
CelII 75 GCTCAGC GC^T[ACGT]AGC
ClaI 875 ATCGAT AT^CGAT
DsaI 93 CCGCGG C^C[GA][CT]GG
EaeI 128 CGGCCG [CT]^GGCC[GA]
EagI 128 CGGCCG C^GGCCG
EarI 598 CTCTTC CTCTTC(1/4)
EclXI 128 CGGCCG C^GGCCG
Eco52I 128 CGGCCG C^GGCCG
Eco81I 432 CCTTAGG CC^T[ACGT]AGG
EcoNI 15 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 403 GGTCACC G^GT[ACGT]ACC
EcoT14I 945 CCTTGG C^C[AT][AT]GG
EcoT22I 905 ATGCAT ATGCA^T
FbaI 990 TGATCA T^GATCA
HgaI 100 GACGC GACGC(5/10)
HindIII 495 AAGCTT A^AGCTT
Ksp632I 598 CTCTTC CTCTTC(1/4)
KspI 93 CCGCGG CCGC^GG
MaeI 283 CTAG C^TAG
MaeII 197 ACGT A^CGT
MamI 457 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NciI 1019 CCGGG CC^[GC]GG
NdeI 380 CATATG CA^TATG
NlaIV 150 GGCTCC GG[ACGT]^[ACGT]CC
NruI 300 TCGCGA TCG^CGA
NsiI 905 ATGCAT ATGCA^T
PshBI 1159 ATTAAT AT^TAAT
SacI 356 GAGCTC GAGCT^C
SacII 93 CCGCGG CCGC^GG
StyI 945 CCTTGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 327 GTAC GT^AC
1095 GTAC
AspHI 88 GAGCAC G[AT]GC[AT]^C
356 GAGCTC
BglII 702 AGATCT A^GATCT
1119 AGATCT
BmyI 88 GAGCAC G[AGT]GC[ACT]^C
356 GAGCTC
BpmI 7 CTGGAG CTGGAG(16/14)
16 CTGGAG
BsiHKAI 88 GAGCAC G[AT]GC[AT]^C
356 GAGCTC
BsiYI 15 CCTGGAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
179 CCCTCGACTGG
BslI 15 CCTGGAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
179 CCCTCGACTGG
Bsp1286I 88 GAGCAC G[AGT]GC[ACT]^C
356 GAGCTC
BstF5I 626 CATCC GGATG(2/0)
252 GGATG
BstYI 702 AGATCT [GA]^GATC[CT]
1119 AGATCT
Cfr13I 201 GGACC G^G[ACGT]CC
396 GGCCC
EcoRI 899 GAATTC G^AATTC
982 GAATTC
FokI 626 CATCC GGATG(9/13)
252 GGATG
HapII 336 CCGG C^CGG
1019 CCGG
HpaII 336 CCGG C^CGG
1019 CCGG
HphI 343 GGTGA GGTGA(8/7)
405 TCACC
MflI 702 AGATCT [GA]^GATC[CT]
1119 AGATCT
MspI 336 CCGG C^CGG
1019 CCGG
RsaI 327 GTAC GT^AC
1095 GTAC
Sau96I 201 GGACC G^G[ACGT]CC
396 GGCCC
SfaNI 268 GATGC GCATC(5/9)
304 GATGC
TaqI 182 TCGA T^CGA
876 TCGA
TspRI 609 CACTG CA[GC]TG[ACGT][ACGT]^
1030 CACTG
TthHB8I 182 TCGA T^CGA
876 TCGA
XhoII 702 AGATCT [GA]^GATC[CT]
1119 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AccII AciI AcsI AluI AlwNI
ApoI BbvI BsaJI BseRI BsmAI
BsrI BssKI BstNI BstUI CfoI
DdeI DpnII EcoRII Fnu4HI HaeIII
HhaI HinP1I HinfI ItaI MaeIII
MboI MboII MnlI MseI MspA1I
MvaI MvnI MwoI NdeII NlaIII
PvuII Sau3AI ScrFI TfiI Tru9I
TseI Tsp45I Tsp509I