Restriction map for
pF1KA0138
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# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AgeI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspI AviII AvrII BamHI
BbvCI BcgI BciVI BfrI BglII
BlnI BsaBI BsiWI BsmI Bsp1407I
BspHI BspLU11I BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CpoI DraI DrdI
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoT22I FseI FspI HpaI KpnI
MamI MfeI MluI MunI NdeI
NotI NruI NsiI NspV PacI
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SapI
SbfI ScaI SexAI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I XcmI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AcyI 2138 GGCGCC G[GA]^CG[CT]C
AhdI 1828 GACATCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2023 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1272 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1828 GACATCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 2138 GGCGCC GGCGC^C
BbrPI 2743 CACGTG CAC^GTG
BclI 1131 TGATCA T^GATCA
BsaHI 2138 GGCGCC G[GA]^CG[CT]C
BsmBI 329 GAGACG CGTCTC(1/5)
BspDI 106 ATCGAT AT^CGAT
BsrDI 2245 GCAATG GCAATG(2/0)
BsrFI 942 GCCGGC [GA]^CCGG[CT]
BstXI 2379 CCACCGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 942 GCCGGC [GA]^CCGG[CT]
ClaI 106 ATCGAT AT^CGAT
EagI 2442 CGGCCG C^GGCCG
Eam1105I 1828 GACATCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2442 CGGCCG C^GGCCG
Eco52I 2442 CGGCCG C^GGCCG
EcoRV 2258 GATATC GAT^ATC
FbaI 1131 TGATCA T^GATCA
Hin1I 2138 GGCGCC G[GA]^CG[CT]C
KasI 2138 GGCGCC G^GCGCC
NaeI 942 GCCGGC GCC^GGC
NarI 2138 GGCGCC GG^CGCC
NgoMIV 942 GCCGGC G^CCGGC
NheI 2611 GCTAGC G^CTAGC
PmaCI 2743 CACGTG CAC^GTG
PmlI 2743 CACGTG CAC^GTG
SfiI 2660 GGCCCTCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2138 GGCGCC GGC^GCC
XbaI 1672 TCTAGA T^CTAGA
XmnI 1272 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 36 enzymes) #
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AccI 1365 GTCGAC GT^[AC][GT]AC
1522 GTCGAC
AccIII 603 TCCGGA T^CCGGA
792 TCCGGA
AfaI 2325 GTAC GT^AC
2835 GTAC
ApaI 33 GGGCCC GGGCC^C
2659 GGGCCC
BglI 927 GCCTGTAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2661 GCCCTCGGGGC
BlpI 87 GCTCAGC GC^T[ACGT]AGC
523 GCTCAGC
Bpu1102I 87 GCTCAGC GC^T[ACGT]AGC
523 GCTCAGC
BsaAI 493 TACGTG [CT]AC^GT[GA]
2743 CACGTG
BsaI 2312 GAGACC GGTCTC(1/5)
2375 GAGACC
BsaWI 603 TCCGGA [AT]^CCGG[AT]
792 TCCGGA
BseAI 603 TCCGGA T^CCGGA
792 TCCGGA
BsgI 500 CTGCAC GTGCAG(16/14)
2264 GTGCAG
BsiEI 1712 CGGTCG CG[GA][CT]^CG
2442 CGGCCG
BspEI 603 TCCGGA T^CCGGA
792 TCCGGA
BspMI 365 ACCTGC ACCTGC(4/8)
1766 GCAGGT
CelII 87 GCTCAGC GC^T[ACGT]AGC
523 GCTCAGC
DraIII 2720 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2743 CACGTGGTG
EcoT14I 2412 CCATGG C^C[AT][AT]GG
2594 CCATGG
HindIII 754 AAGCTT A^AGCTT
1471 AAGCTT
KspI 887 CCGCGG CCGC^GG
2688 CCGCGG
MaeII 494 ACGT A^CGT
2744 ACGT
MroI 603 TCCGGA T^CCGGA
792 TCCGGA
NcoI 2412 CCATGG C^CATGG
2594 CCATGG
NspI 537 GCATGC [GA]CATG^[CT]
2072 GCATGC
PaeR7I 1951 CTCGAG C^TCGAG
2008 CTCGAG
PpuMI 770 AGGACCT [GA]G^G[AT]CC[CT]
2424 AGGACCC
PstI 367 CTGCAG CTGCA^G
2002 CTGCAG
PvuII 505 CAGCTG CAG^CTG
2152 CAGCTG
RsaI 2325 GTAC GT^AC
2835 GTAC
SacII 887 CCGCGG CCGC^GG
2688 CCGCGG
SalI 1365 GTCGAC G^TCGAC
1522 GTCGAC
SphI 537 GCATGC GCATG^C
2072 GCATGC
StuI 983 AGGCCT AGG^CCT
1955 AGGCCT
StyI 2412 CCATGG C^C[AT][AT]GG
2594 CCATGG
TspRI 426 CAGTG CA[GC]TG[ACGT][ACGT]^
1119 CAGTG
XhoI 1951 CTCGAG C^TCGAG
2008 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AluI AlwI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TthHB8I Van91I XhoII XmaCI XmaI