Restriction map for
pF1KA0209
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# NO CUT ENZYMES ( 72 enzymes) #
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AatII AclI AgeI Aor51HI ApaI
AscI AseI AsnI AspI AvrII
BbeI BbrPI BcgI BclI BlnI
BsiWI BspDI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI EagI EclXI
Eco47III Eco52I EcoRV EcoT22I FbaI
FseI HincII HindII HpaI KasI
MfeI MluI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SalI SbfI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Acc65I 3665 GGTACC G^GTACC
AccI 4357 GTAGAC GT^[AC][GT]AC
AccIII 3308 TCCGGA T^CCGGA
AflII 919 CTTAAG C^TTAAG
AhdI 5470 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 3665 GGTACC G^GTACC
AspEI 5470 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 4343 TGCGCA TGC^GCA
BanI 3665 GGTACC G^G[CT][GA]CC
BbvCI 5346 CCTCAGC CCTCAGC(-5/-2)
BfrI 919 CTTAAG C^TTAAG
BglI 1085 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3932 AGATCT A^GATCT
BsaAI 4312 TACGTG [CT]AC^GT[GA]
BseAI 3308 TCCGGA T^CCGGA
BsiEI 2245 CGGTCG CG[GA][CT]^CG
BspEI 3308 TCCGGA T^CCGGA
BspLU11I 4457 ACATGT A^CATGT
BsrBI 28 GAGCGG CCGCTC(-3/-3)
BsrFI 3818 ACCGGC [GA]^CCGG[CT]
BssSI 3412 CACGAG CACGAG(-5/-1)
Cfr10I 3818 ACCGGC [GA]^CCGG[CT]
DraI 2542 TTTAAA TTT^AAA
Eam1105I 5470 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 4953 GAATTC G^AATTC
FspI 4343 TGCGCA TGC^GCA
HaeII 5292 GGCGCT [GA]GCGC^[CT]
HindIII 3226 AAGCTT A^AGCTT
KpnI 3665 GGTACC GGTAC^C
KspI 4089 CCGCGG CCGC^GG
MroI 3308 TCCGGA T^CCGGA
NcoI 2774 CCATGG C^CATGG
PflMI 2631 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 4089 CCGCGG CCGC^GG
SapI 717 GCTCTTC GCTCTTC(1/4)
ScaI 2648 AGTACT AGT^ACT
SphI 5211 GCATGC GCATG^C
Van91I 2631 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AcyI 5022 GACGCC G[GA]^CG[CT]C
5232 GGCGTC
Alw44I 942 GTGCAC G^TGCAC
4453 GTGCAC
ApaLI 942 GTGCAC G^TGCAC
4453 GTGCAC
Asp700I 1068 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4641 GAAGGCCTTC
BamHI 1511 GGATCC G^GATCC
4430 GGATCC
BfaI 772 CTAG C^TAG
3046 CTAG
BsaHI 5022 GACGCC G[GA]^CG[CT]C
5232 GGCGTC
Bsp1407I 3656 TGTACA T^GTACA
3671 TGTACA
BsrGI 3656 TGTACA T^GTACA
3671 TGTACA
BstAPI 3441 GCAGTACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3599 GCATGAGCTGC
BstEII 1140 GGTGACC G^GT[ACGT]ACC
2842 GGTGACC
BstPI 1140 GGTGACC G^GT[ACGT]ACC
2842 GGTGACC
EcoNI 2367 CCTTTTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5067 CCTGCCTGAGG
EcoO65I 1140 GGTGACC G^GT[ACGT]ACC
2842 GGTGACC
FauI 5342 CCCGC CCCGC(4/6)
4091 GCGGG
Hin1I 5022 GACGCC G[GA]^CG[CT]C
5232 GGCGTC
MaeI 772 CTAG C^TAG
3046 CTAG
PshAI 1535 GACATCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4922 GACAGATGTC
SspBI 3656 TGTACA T^GTACA
3671 TGTACA
XmnI 1068 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4641 GAAGGCCTTC
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# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
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AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AspHI AvaI AvaII BalI BanII
BbsI BbvI BciVI BcnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaBI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspHI
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DraIII DrdI DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RcaI RsaI
SacI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmaCI XmaI