Restriction map for
pF1KA0249
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# NO CUT ENZYMES ( 100 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AgeI Alw44I Aor51HI ApaLI
AscI Asp718I AviII BalI BamHI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglII BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BsrBI BsrDI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
CelII ClaI CpoI DrdI Eco47III
EcoNI EcoO65I EcoT22I FbaI FspI
HgaI HincII HindII HpaI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MunI MvnI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI XcmI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 1144 GTAGAC GT^[AC][GT]AC
AcyI 1113 GGCGCC G[GA]^CG[CT]C
AlwNI 1379 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1615 CCTAAGG CC^T[ACGT]AGG
AseI 2511 ATTAAT AT^TAAT
AsnI 2511 ATTAAT AT^TAAT
Asp700I 289 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 955 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 853 CCTAGG C^CTAGG
BbeI 1113 GGCGCC GGCGC^C
BglI 1789 GCCAGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 853 CCTAGG C^CTAGG
BpmI 739 CTGGAG CTGGAG(16/14)
BsaHI 1113 GGCGCC G[GA]^CG[CT]C
BspLU11I 538 ACATGT A^CATGT
Bsu36I 1615 CCTAAGG CC^T[ACGT]AGG
DraI 343 TTTAAA TTT^AAA
DraIII 1365 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1615 CCTAAGG CC^T[ACGT]AGG
EcoRI 2604 GAATTC G^AATTC
EcoRV 2377 GATATC GAT^ATC
FauI 1421 GCGGG CCCGC(4/6)
FseI 1793 GGCCGGCC GGCCGG^CC
HaeII 1113 GGCGCC [GA]GCGC^[CT]
Hin1I 1113 GGCGCC G[GA]^CG[CT]C
KasI 1113 GGCGCC G^GCGCC
NarI 1113 GGCGCC GG^CGCC
PaeR7I 1812 CTCGAG C^TCGAG
PflFI 955 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1286 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1194 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 2511 ATTAAT AT^TAAT
ScaI 1054 AGTACT AGT^ACT
SfoI 1113 GGCGCC GGC^GCC
SmaI 2276 CCCGGG CCC^GGG
SspI 2484 AATATT AAT^ATT
Tth111I 955 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1286 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1230 TCTAGA T^CTAGA
XhoI 1812 CTCGAG C^TCGAG
XmaCI 2276 CCCGGG C^CCGGG
XmaI 2276 CCCGGG C^CCGGG
XmnI 289 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AflIII 414 ACACGT A^C[GA][CT]GT
538 ACATGT
AhdI 711 GACAGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1147 GACTCGCCGTC
ApaI 575 GGGCCC GGGCC^C
2280 GGGCCC
AspEI 711 GACAGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1147 GACTCGCCGTC
BbsI 532 GAAGAC GAAGAC(2/6)
952 GAAGAC
BfaI 854 CTAG C^TAG
1231 CTAG
BpuAI 532 GAAGAC GAAGAC(2/6)
952 GAAGAC
BsaI 694 GAGACC GGTCTC(1/5)
2651 GAGACC
BsiEI 1781 CGGCCG CG[GA][CT]^CG
1796 CGGCCG
BstAPI 517 GCATCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1364 GCACCGAGTGC
CfoI 1114 GCGC GCG^C
1346 GCGC
EagI 1781 CGGCCG C^GGCCG
1796 CGGCCG
Eam1105I 711 GACAGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1147 GACTCGCCGTC
EclXI 1781 CGGCCG C^GGCCG
1796 CGGCCG
Eco52I 1781 CGGCCG C^GGCCG
1796 CGGCCG
HhaI 1114 GCGC GCG^C
1346 GCGC
HinP1I 1114 GCGC G^CGC
1346 GCGC
HindIII 254 AAGCTT A^AGCTT
292 AAGCTT
MaeI 854 CTAG C^TAG
1231 CTAG
MslI 1848 CATCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2211 CATCGGCATG
NaeI 1779 GCCGGC GCC^GGC
1794 GCCGGC
NcoI 947 CCATGG C^CATGG
1388 CCATGG
NgoMIV 1779 GCCGGC G^CCGGC
1794 GCCGGC
NspI 538 ACATGT [GA]CATG^[CT]
2574 GCATGT
PstI 527 CTGCAG CTGCA^G
1352 CTGCAG
SmlI 1048 CTTGAG C^T[CT][GA]AG
1812 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaI AvaII BanI
BanII BbvI BcnI BmyI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstXI BstYI Cac8I Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII