Restriction map for
pF1KA0254
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# NO CUT ENZYMES ( 103 enzymes) #
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AatII AccIII AclI AflII AgeI
AhdI ApaI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BbeI BbrPI BcgI BciVI BfrI
BglI BlnI BsaAI BsaBI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DrdI
Eam1105I EarI EcoO65I EcoRV EcoT22I
FauI FseI FspI HindIII HpaI
KasI Ksp632I KspI MamI MfeI
MroI MunI NarI NcoI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 350 GGTACC G^GTACC
AccI 224 GTCGAC GT^[AC][GT]AC
AccII 635 CGCG CG^CG
AcyI 701 GACGCC G[GA]^CG[CT]C
Alw44I 604 GTGCAC G^TGCAC
AocI 274 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2612 AGCGCT AGC^GCT
ApaLI 604 GTGCAC G^TGCAC
Asp718I 350 GGTACC G^GTACC
AvaI 1622 CCCGAG C^[CT]CG[GA]G
BalI 254 TGGCCA TGG^CCA
BamHI 776 GGATCC G^GATCC
BclI 749 TGATCA T^GATCA
BcnI 579 CCGGG CC^[GC]GG
BsaHI 701 GACGCC G[GA]^CG[CT]C
BsaWI 697 ACCGGA [AT]^CCGG[AT]
BsiEI 1435 CGGCCG CG[GA][CT]^CG
BsoBI 1622 CCCGAG C^[CT]CG[GA]G
BspHI 1160 TCATGA T^CATGA
BssSI 790 CTCGTG CACGAG(-5/-1)
BstUI 635 CGCG CG^CG
BstXI 2608 CCACAGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 274 CCTGAGG CC^T[ACGT]AGG
CfoI 2613 GCGC GCG^C
DsaI 2680 CCACGG C^C[GA][CT]GG
EagI 1435 CGGCCG C^GGCCG
EclXI 1435 CGGCCG C^GGCCG
Eco47III 2612 AGCGCT AGC^GCT
Eco52I 1435 CGGCCG C^GGCCG
Eco81I 274 CCTGAGG CC^T[ACGT]AGG
FbaI 749 TGATCA T^GATCA
HaeII 2612 AGCGCT [GA]GCGC^[CT]
HgaI 701 GACGC GACGC(5/10)
HhaI 2613 GCGC GCG^C
Hin1I 701 GACGCC G[GA]^CG[CT]C
HinP1I 2613 GCGC G^CGC
KpnI 350 GGTACC GGTAC^C
MluI 634 ACGCGT A^CGCGT
MluNI 254 TGGCCA TGG^CCA
MscI 254 TGGCCA TGG^CCA
MseI 2599 TTAA T^TAA
MvnI 635 CGCG CG^CG
NaeI 2792 GCCGGC GCC^GGC
NciI 579 CCGGG CC^[GC]GG
NgoMIV 2792 GCCGGC G^CCGGC
NheI 2158 GCTAGC G^CTAGC
RcaI 1160 TCATGA T^CATGA
SacI 1473 GAGCTC GAGCT^C
SalI 224 GTCGAC G^TCGAC
SexAI 1521 ACCTGGT A^CC[AT]GGT
StuI 1302 AGGCCT AGG^CCT
Tru9I 2599 TTAA T^TAA
XbaI 2020 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 19 enzymes) #
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BlpI 1535 GCTCAGC GC^T[ACGT]AGC
2796 GCTGAGC
Bpu1102I 1535 GCTCAGC GC^T[ACGT]AGC
2796 GCTGAGC
BsrDI 167 CATTGC GCAATG(2/0)
731 GCAATG
BsrFI 1308 GCCGGT [GA]^CCGG[CT]
2792 GCCGGC
CelII 1535 GCTCAGC GC^T[ACGT]AGC
2796 GCTGAGC
Cfr10I 1308 GCCGGT [GA]^CCGG[CT]
2792 GCCGGC
DraIII 1729 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
2204 CACTAAGTG
EaeI 254 TGGCCA [CT]^GGCC[GA]
1435 CGGCCG
EcoNI 561 CCTCTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2368 CCTCCCAAAGG
EcoRI 1323 GAATTC G^AATTC
2881 GAATTC
EcoT14I 806 CCAAGG C^C[AT][AT]GG
2862 CCTTGG
HincII 136 GTCAAC GT[CT]^[GA]AC
224 GTCGAC
HindII 136 GTCAAC GT[CT]^[GA]AC
224 GTCGAC
MaeII 140 ACGT A^CGT
2379 ACGT
MslI 315 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1155 CATGCTCATG
NspI 727 ACATGC [GA]CATG^[CT]
2528 ACATGC
PflMI 705 CCATGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1224 CCAGGCTCTGG
StyI 806 CCAAGG C^C[AT][AT]GG
2862 CCTTGG
Van91I 705 CCATGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1224 CCAGGCTCTGG
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaII
BanI BanII BbsI BbvCI BbvI
BfaI BglII BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MflI MnlI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII