Restriction map for
pF1KA0456
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# NO CUT ENZYMES ( 82 enzymes) #
##################################
Acc65I AccII AflII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AviII AvrII BanI BbeI
BbvCI BfrI BglI BglII BlnI
BsiEI BsiWI BspDI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
ClaI CpoI DraIII EagI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV FseI FspI HpaI KasI
KpnI KspI MfeI MluI MunI
MvnI NarI NdeI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI PvuI RsrII SacII
SalI SapI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspI StuI SwaI XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 3 GACGTC GACGT^C
AclI 634 AACGTT AA^CGTT
AcyI 3 GACGTC G[GA]^CG[CT]C
AhdI 1966 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2522 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2815 CCTGAGG CC^T[ACGT]AGG
Asp700I 2028 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1966 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2365 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 224 TGGCCA TGG^CCA
BamHI 1588 GGATCC G^GATCC
BbrPI 1987 CACGTG CAC^GTG
BcgI 2193 CGAGGCCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1532 GTATCC GTATCC(6/5)
BsaBI 2482 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3 GACGTC G[GA]^CG[CT]C
BsaI 1275 GGTCTC GGTCTC(1/5)
BsmBI 5 CGTCTC CGTCTC(1/5)
BsmI 942 GAATGC GAATGC(1/-1)
BspMI 1778 ACCTGC ACCTGC(4/8)
BsrBI 2444 GAGCGG CCGCTC(-3/-3)
BssSI 2166 CACGAG CACGAG(-5/-1)
BstXI 2045 CCAGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2815 CCTGAGG CC^T[ACGT]AGG
DraI 415 TTTAAA TTT^AAA
DrdI 2812 GACCCTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1966 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2815 CCTGAGG CC^T[ACGT]AGG
EcoT22I 782 ATGCAT ATGCA^T
Hin1I 3 GACGTC G[GA]^CG[CT]C
MamI 2482 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 224 TGGCCA TGG^CCA
MscI 224 TGGCCA TGG^CCA
NaeI 2303 GCCGGC GCC^GGC
NgoMIV 2303 GCCGGC G^CCGGC
NheI 2611 GCTAGC G^CTAGC
NsiI 782 ATGCAT ATGCA^T
PflFI 2365 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1987 CACGTG CAC^GTG
PmlI 1987 CACGTG CAC^GTG
Psp1406I 634 AACGTT AA^CGTT
PvuII 2454 CAGCTG CAG^CTG
SbfI 1779 CCTGCAGG CCTGCA^GG
SmlI 3001 CTCAAG C^T[CT][GA]AG
Sse8387I 1779 CCTGCAGG CCTGCA^GG
Tth111I 2365 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 283 CCAGTCAACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2028 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 2065 GTCTAC GT^[AC][GT]AC
3189 GTCTAC
AcsI 16 AAATTC [GA]^AATT[CT]
1571 GAATTT
AflIII 503 ACATGT A^C[GA][CT]GT
654 ACATGT
ApaI 1933 GGGCCC GGGCC^C
2058 GGGCCC
ApoI 16 AAATTC [GA]^AATT[CT]
1571 GAATTT
AvaI 2231 CCCGAG C^[CT]CG[GA]G
3010 CCCGAG
BclI 828 TGATCA T^GATCA
2000 TGATCA
BlpI 76 GCTCAGC GC^T[ACGT]AGC
2568 GCTGAGC
Bpu1102I 76 GCTCAGC GC^T[ACGT]AGC
2568 GCTGAGC
BsaAI 1987 CACGTG [CT]AC^GT[GA]
2215 TACGTG
BsgI 1795 CTGCAC GTGCAG(16/14)
2986 CTGCAC
BsoBI 2231 CCCGAG C^[CT]CG[GA]G
3010 CCCGAG
BspLU11I 503 ACATGT A^CATGT
654 ACATGT
BsrFI 599 ACCGGC [GA]^CCGG[CT]
2303 GCCGGC
BstAPI 129 GCAACTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
775 GCAACCAATGC
CelII 76 GCTCAGC GC^T[ACGT]AGC
2568 GCTGAGC
Cfr10I 599 ACCGGC [GA]^CCGG[CT]
2303 GCCGGC
FbaI 828 TGATCA T^GATCA
2000 TGATCA
HaeII 980 AGCGCC [GA]GCGC^[CT]
3031 AGCGCC
HincII 286 GTCAAC GT[CT]^[GA]AC
3166 GTCAAC
HindII 286 GTCAAC GT[CT]^[GA]AC
3166 GTCAAC
HindIII 1696 AAGCTT A^AGCTT
2787 AAGCTT
NspI 503 ACATGT [GA]CATG^[CT]
654 ACATGT
PflMI 1665 CCATGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2521 CCAGAATCTGG
SexAI 311 ACCAGGT A^CC[AT]GGT
1967 ACCAGGT
Van91I 1665 CCATGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2521 CCAGAATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccIII AciI AfaI AluI AlwI
AspHI AvaII BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BsaWI BseAI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspEI BspHI BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MroI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
PpuMI PstI RcaI RsaI SacI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII