Restriction map for
pF1KA0569
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AgeI Alw44I ApaI ApaLI AscI
AseI AsnI Asp718I AspI AviII
AvrII BalI BbeI BclI BfrI
BglI BglII BlnI BsaHI BseAI
BsgI BsiWI Bsp1407I BspDI BspEI
BsrFI BsrGI Bst1107I BstBI BstEII
BstPI BstZ17I Cfr10I ClaI CpoI
DraI DrdI EagI EclXI Eco52I
EcoO65I EcoRV FbaI FseI FspI
HgaI Hin1I HincII HindII HpaI
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MunI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PflFI PflMI PinAI
PmeI PshBI PvuI PvuII RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SphI
Sse8387I SspBI SwaI Tth111I Van91I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 2089 GTCTAC GT^[AC][GT]AC
AlwNI 101 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
BanI 1558 GGTGCC G^G[CT][GA]CC
BbrPI 217 CACGTG CAC^GTG
BbvCI 139 GCTGAGG CCTCAGC(-5/-2)
BciVI 3516 GGATAC GTATCC(6/5)
BlpI 2437 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2437 GCTGAGC GC^T[ACGT]AGC
BsaBI 3517 GATACGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2189 GGTCTC GGTCTC(1/5)
BsaWI 1539 TCCGGT [AT]^CCGG[AT]
BsiEI 648 CGACCG CG[GA][CT]^CG
BsmBI 175 GAGACG CGTCTC(1/5)
BsmI 408 GAATGC GAATGC(1/-1)
BspHI 1848 TCATGA T^CATGA
BsrDI 2034 CATTGC GCAATG(2/0)
BssHII 3283 GCGCGC G^CGCGC
CelII 2437 GCTGAGC GC^T[ACGT]AGC
DraII 2110 AGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 852 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 3566 CCATGG C^C[GA][CT]GG
EaeI 1023 TGGCCG [CT]^GGCC[GA]
EcoNI 2046 CCTTCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 2110 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 905 GAATTC G^AATTC
EcoT22I 2996 ATGCAT ATGCA^T
MamI 3517 GATACGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 2866 CAGCGG C[AC]G^C[GT]G
NcoI 3566 CCATGG C^CATGG
NsiI 2996 ATGCAT ATGCA^T
PaeR7I 766 CTCGAG C^TCGAG
PmaCI 217 CACGTG CAC^GTG
PmlI 217 CACGTG CAC^GTG
PpuMI 2110 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 2947 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1840 CTGCAG CTGCA^G
RcaI 1848 TCATGA T^CATGA
SacI 2428 GAGCTC GAGCT^C
SapI 2689 GCTCTTC GCTCTTC(1/4)
ScaI 2096 AGTACT AGT^ACT
SnaBI 1676 TACGTA TAC^GTA
SpeI 1234 ACTAGT A^CTAGT
XhoI 766 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AclI 950 AACGTT AA^CGTT
1799 AACGTT
AhdI 1960 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2911 GACTACATGTC
Aor51HI 662 AGCGCT AGC^GCT
3185 AGCGCT
AspEI 1960 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2911 GACTACATGTC
BamHI 1476 GGATCC G^GATCC
3522 GGATCC
BcgI 468 CGATGGTCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
758 GCACCCAGCTCG
BsaAI 217 CACGTG [CT]AC^GT[GA]
1676 TACGTA
BspLU11I 1964 ACATGT A^CATGT
2915 ACATGT
BsrBI 769 GAGCGG CCGCTC(-3/-3)
3259 GAGCGG
BstAPI 807 GCACCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1981 GCATACTATGC
BstXI 158 CCAACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2033 CCATTGCTGTGG
BstYI 1476 GGATCC [GA]^GATC[CT]
3522 GGATCC
Eam1105I 1960 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2911 GACTACATGTC
Eco47III 662 AGCGCT AGC^GCT
3185 AGCGCT
FauI 3246 GCGGG CCCGC(4/6)
3261 GCGGG
HaeII 662 AGCGCT [GA]GCGC^[CT]
3185 AGCGCT
HindIII 127 AAGCTT A^AGCTT
1610 AAGCTT
MflI 1476 GGATCC [GA]^GATC[CT]
3522 GGATCC
NdeI 775 CATATG CA^TATG
2800 CATATG
Psp1406I 950 AACGTT AA^CGTT
1799 AACGTT
SspI 1519 AATATT AAT^ATT
2302 AATATT
StuI 866 AGGCCT AGG^CCT
2922 AGGCCT
TfiI 303 GATTC G^A[AT]TC
3222 GAATC
XcmI 446 CCAAAAGAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1415 CCAGGCAAAAAATGG
XhoII 1476 GGATCC [GA]^GATC[CT]
3522 GGATCC
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# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AocI ApoI Asp700I
AspHI AvaI AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BssSI BstF5I
BstNI BstUI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII EarI Eco81I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XmnI