Restriction map for
pF1KA0596
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# NO CUT ENZYMES ( 74 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp718I AspEI AspI BfrI
BlpI Bpu1102I BsaWI BseAI BsiEI
BsiWI BspDI BspEI BspHI BspLU11I
BssHII BssSI Bst1107I BstBI BstZ17I
CelII ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI FseI
HindIII HpaI KpnI MfeI MluI
MroI MunI NaeI NdeI NgoMIV
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SalI SfiI
SfuI SgrAI SnaBI SpeI SphI
SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcyI 2189 GGCGCC G[GA]^CG[CT]C
AflIII 482 ACGTGT A^C[GA][CT]GT
Asp700I 2392 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 1428 GGATCC G^GATCC
BbeI 2189 GGCGCC GGCGC^C
BbrPI 1834 CACGTG CAC^GTG
BclI 1751 TGATCA T^GATCA
BglI 2745 GCCCCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1834 CACGTG [CT]AC^GT[GA]
BsaHI 2189 GGCGCC G[GA]^CG[CT]C
BsaI 3372 GAGACC GGTCTC(1/5)
Bsp1407I 1085 TGTACA T^GTACA
BsrGI 1085 TGTACA T^GTACA
BstAPI 3011 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 114 GGTGACC G^GT[ACGT]ACC
BstPI 114 GGTGACC G^GT[ACGT]ACC
DraI 1960 TTTAAA TTT^AAA
EcoO65I 114 GGTGACC G^GT[ACGT]ACC
EcoRV 3771 GATATC GAT^ATC
EcoT22I 1415 ATGCAT ATGCA^T
FbaI 1751 TGATCA T^GATCA
Hin1I 2189 GGCGCC G[GA]^CG[CT]C
KasI 2189 GGCGCC G^GCGCC
KspI 4278 CCGCGG CCGC^GG
NarI 2189 GGCGCC GG^CGCC
NsiI 1415 ATGCAT ATGCA^T
NspI 335 ACATGC [GA]CATG^[CT]
PmaCI 1834 CACGTG CAC^GTG
PmlI 1834 CACGTG CAC^GTG
PshAI 1597 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 4278 CCGCGG CCGC^GG
SapI 3100 GAAGAGC GCTCTTC(1/4)
SbfI 2643 CCTGCAGG CCTGCA^GG
ScaI 308 AGTACT AGT^ACT
SexAI 3884 ACCTGGT A^CC[AT]GGT
SfoI 2189 GGCGCC GGC^GCC
SmaI 2927 CCCGGG CCC^GGG
Sse8387I 2643 CCTGCAGG CCTGCA^GG
SspBI 1085 TGTACA T^GTACA
SspI 1915 AATATT AAT^ATT
StuI 1217 AGGCCT AGG^CCT
XmaCI 2927 CCCGGG C^CCGGG
XmaI 2927 CCCGGG C^CCGGG
XmnI 2392 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccI 1189 GTCTAC GT^[AC][GT]AC
2863 GTCTAC
AccII 1435 CGCG CG^CG
4279 CGCG
AcsI 601 AAATTC [GA]^AATT[CT]
2128 AAATTT
Alw44I 1513 GTGCAC G^TGCAC
3595 GTGCAC
ApaLI 1513 GTGCAC G^TGCAC
3595 GTGCAC
ApoI 601 AAATTC [GA]^AATT[CT]
2128 AAATTT
AviII 68 TGCGCA TGC^GCA
1791 TGCGCA
AvrII 3999 CCTAGG C^CTAGG
4063 CCTAGG
BcgI 2437 GCACTGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3361 GCACTGATGTCG
BciVI 2895 GGATAC GTATCC(6/5)
1029 GTATCC
BglII 49 AGATCT A^GATCT
2829 AGATCT
BlnI 3999 CCTAGG C^CTAGG
4063 CCTAGG
BsaBI 50 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2205 GATGACCATC
BsmBI 3514 CGTCTC CGTCTC(1/5)
3722 CGTCTC
BsrBI 663 CCGCTC CCGCTC(-3/-3)
1446 CCGCTC
BstUI 1435 CGCG CG^CG
4279 CGCG
DrdI 469 GACATGATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
540 GACAGCAGTGTC
EcoNI 3643 CCTTCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4215 CCTGAGCCAGG
FspI 68 TGCGCA TGC^GCA
1791 TGCGCA
HincII 15 GTCAAC GT[CT]^[GA]AC
478 GTCAAC
HindII 15 GTCAAC GT[CT]^[GA]AC
478 GTCAAC
MamI 50 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2205 GATGACCATC
MvnI 1435 CGCG CG^CG
4279 CGCG
NheI 979 GCTAGC G^CTAGC
1614 GCTAGC
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# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII