Restriction map for
pF1KA0603
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# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AflII AgeI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspI AviII AvrII BalI BamHI
BfrI BglI BlnI BsaBI BsiEI
BsiWI BspLU11I Bst1107I BstXI BstZ17I
DraIII DrdI EagI EclXI Eco47III
Eco52I EcoT22I FseI FspI HpaI
KpnI MamI MluI MluNI MscI
NdeI NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmeI
PshBI PvuI SbfI ScaI SexAI
SfiI SnaBI Sse8387I SwaI Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AclI 1872 AACGTT AA^CGTT
Alw44I 1588 GTGCAC G^TGCAC
ApaLI 1588 GTGCAC G^TGCAC
BbeI 225 GGCGCC GGCGC^C
BbrPI 1585 CACGTG CAC^GTG
BcgI 927 GCAGGAGTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3019 GGATAC GTATCC(6/5)
BclI 425 TGATCA T^GATCA
BglII 3204 AGATCT A^GATCT
BsaAI 1585 CACGTG [CT]AC^GT[GA]
BsmBI 63 CGTCTC CGTCTC(1/5)
BsmI 17 GCATTC GAATGC(1/-1)
Bsp1407I 2057 TGTACA T^GTACA
BspDI 664 ATCGAT AT^CGAT
BspHI 3189 TCATGA T^CATGA
BsrBI 892 GAGCGG CCGCTC(-3/-3)
BsrGI 2057 TGTACA T^GTACA
BssHII 260 GCGCGC G^CGCGC
BstAPI 1374 GCACTCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 627 GGTGACC G^GT[ACGT]ACC
BstPI 627 GGTGACC G^GT[ACGT]ACC
ClaI 664 ATCGAT AT^CGAT
CpoI 732 CGGTCCG CG^G[AT]CCG
DraI 1144 TTTAAA TTT^AAA
EcoO65I 627 GGTGACC G^GT[ACGT]ACC
EcoRI 2157 GAATTC G^AATTC
EcoRV 1150 GATATC GAT^ATC
FbaI 425 TGATCA T^GATCA
HincII 2768 GTCGAC GT[CT]^[GA]AC
HindII 2768 GTCGAC GT[CT]^[GA]AC
HindIII 2145 AAGCTT A^AGCTT
KasI 225 GGCGCC G^GCGCC
KspI 3812 CCGCGG CCGC^GG
MfeI 3828 CAATTG C^AATTG
MunI 3828 CAATTG C^AATTG
NaeI 228 GCCGGC GCC^GGC
NarI 225 GGCGCC GG^CGCC
NcoI 2432 CCATGG C^CATGG
NgoMIV 228 GCCGGC G^CCGGC
PmaCI 1585 CACGTG CAC^GTG
PmlI 1585 CACGTG CAC^GTG
PshAI 2963 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1872 AACGTT AA^CGTT
RcaI 3189 TCATGA T^CATGA
RsrII 732 CGGTCCG CG^G[AT]CCG
SacI 2013 GAGCTC GAGCT^C
SacII 3812 CCGCGG CCGC^GG
SalI 2768 GTCGAC G^TCGAC
SfoI 225 GGCGCC GGC^GCC
SpeI 313 ACTAGT A^CTAGT
SspBI 2057 TGTACA T^GTACA
SspI 3418 AATATT AAT^ATT
XcmI 1262 CCAGCGAATCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccI 2165 GTAGAC GT^[AC][GT]AC
2768 GTCGAC
AflIII 1584 ACACGT A^C[GA][CT]GT
1922 ACACGT
AhdI 445 GACCCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3142 GACATGATGTC
AocI 1551 CCTGAGG CC^T[ACGT]AGG
2331 CCTCAGG
AspEI 445 GACCCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3142 GACATGATGTC
BspMI 840 ACCTGC ACCTGC(4/8)
2254 ACCTGC
BsrDI 2261 GCAATG GCAATG(2/0)
2368 GCAATG
BsrFI 228 GCCGGC [GA]^CCGG[CT]
958 ACCGGC
BssSI 979 CACGAG CACGAG(-5/-1)
2902 CTCGTG
Bsu36I 1551 CCTGAGG CC^T[ACGT]AGG
2331 CCTCAGG
Cfr10I 228 GCCGGC [GA]^CCGG[CT]
958 ACCGGC
DsaI 2432 CCATGG C^C[GA][CT]GG
3812 CCGCGG
Eam1105I 445 GACCCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3142 GACATGATGTC
Eco81I 1551 CCTGAGG CC^T[ACGT]AGG
2331 CCTCAGG
EcoNI 423 CCTGATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
798 CCTGCCGGAGG
PflMI 1216 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3684 CCAGGCCTTGG
SapI 3073 GAAGAGC GCTCTTC(1/4)
1071 GCTCTTC
SgrAI 227 CGCCGGCG C[GA]^CCGG[CT]G
957 CACCGGCG
SmaI 82 CCCGGG CCC^GGG
778 CCCGGG
SmlI 654 CTCAAG C^T[CT][GA]AG
1725 CTCAAG
SphI 2892 GCATGC GCATG^C
3519 GCATGC
Van91I 1216 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3684 CCAGGCCTTGG
XmaCI 82 CCCGGG C^CCGGG
778 CCCGGG
XmaI 82 CCCGGG C^CCGGG
778 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
Asp700I AspHI AvaI AvaII BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspEI BsrI BssKI BstBI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MroI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
NspV PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfuI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI