Restriction map for
pF1KA0612
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# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AclI AcsI AflIII
AgeI Alw44I ApaLI ApoI AscI
AseI AsnI Asp718I AvrII BciVI
BlnI BsiWI Bsp1407I BspHI BspLU11I
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I CpoI DraI DrdI
EcoO65I EcoRI EcoRV EcoT22I FseI
HindIII HpaI KpnI KspI MfeI
MluI MunI NdeI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI XbaI
XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 1293 GTCTAC GT^[AC][GT]AC
AccIII 1226 TCCGGA T^CCGGA
AflII 418 CTTAAG C^TTAAG
Aor51HI 404 AGCGCT AGC^GCT
Asp700I 4768 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1729 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1781 GGATCC G^GATCC
BbrPI 589 CACGTG CAC^GTG
BclI 2807 TGATCA T^GATCA
BfrI 418 CTTAAG C^TTAAG
BglII 3752 AGATCT A^GATCT
BsaAI 589 CACGTG [CT]AC^GT[GA]
BsaBI 2802 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1226 TCCGGA T^CCGGA
BspDI 2833 ATCGAT AT^CGAT
BspEI 1226 TCCGGA T^CCGGA
BsrFI 4223 GCCGGC [GA]^CCGG[CT]
Cfr10I 4223 GCCGGC [GA]^CCGG[CT]
ClaI 2833 ATCGAT AT^CGAT
DraIII 2233 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 3573 CGGCCG C^GGCCG
EclXI 3573 CGGCCG C^GGCCG
Eco47III 404 AGCGCT AGC^GCT
Eco52I 3573 CGGCCG C^GGCCG
FbaI 2807 TGATCA T^GATCA
HgaI 678 GACGC GACGC(5/10)
HincII 2496 GTTGAC GT[CT]^[GA]AC
HindII 2496 GTTGAC GT[CT]^[GA]AC
MamI 2802 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1226 TCCGGA T^CCGGA
NaeI 4223 GCCGGC GCC^GGC
NgoMIV 4223 GCCGGC G^CCGGC
NheI 4210 GCTAGC G^CTAGC
PflFI 1729 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 589 CACGTG CAC^GTG
PmlI 589 CACGTG CAC^GTG
PshAI 3928 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 603 CGATCG CGAT^CG
Tth111I 1729 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 4768 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AccII 616 CGCG CG^CG
657 CGCG
AviII 662 TGCGCA TGC^GCA
2990 TGCGCA
BsiEI 603 CGATCG CG[GA][CT]^CG
3573 CGGCCG
BsmBI 194 GAGACG CGTCTC(1/5)
676 GAGACG
BsmI 954 GAATGC GAATGC(1/-1)
1026 GAATGC
BsrDI 513 CATTGC GCAATG(2/0)
3908 CATTGC
BssSI 3250 CACGAG CACGAG(-5/-1)
3562 CTCGTG
BstUI 616 CGCG CG^CG
657 CGCG
FspI 662 TGCGCA TGC^GCA
2990 TGCGCA
MseI 419 TTAA T^TAA
5260 TTAA
MvnI 616 CGCG CG^CG
657 CGCG
NspI 1139 ACATGC [GA]CATG^[CT]
4004 ACATGC
SapI 444 GAAGAGC GCTCTTC(1/4)
4768 GAAGAGC
Tru9I 419 TTAA T^TAA
5260 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AciI AcyI AfaI AhdI AluI
AlwI AlwNI AocI ApaI AspEI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvCI BbvI
BcgI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI Eam1105I EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfiI SfoI SmaI SmlI StuI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI