Restriction map for
pF1KA0621
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII AclI AcyI AflII AflIII
AgeI Alw44I ApaI ApaLI AscI
AseI AsnI Asp700I AvrII BamHI
BbeI BbrPI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BseRI BsiEI BsiWI BspDI
BspLU11I BspMI BsrBI BsrDI BsrFI
BssSI Bst1107I BstEII BstPI BstXI
BstZ17I CelII Cfr10I ClaI CpoI
DraIII EagI EclXI Eco52I EcoNI
EcoO65I EcoRI FauI FbaI FseI
Hin1I KasI KspI MamI MfeI
MluI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspI
PacI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SgrAI SmaI SnaBI
SphI Sse8387I SspI XbaI XcmI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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Acc65I 900 GGTACC G^GTACC
AccII 144 CGCG CG^CG
AccIII 1997 TCCGGA T^CCGGA
AlwNI 24 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 12 AGCGCT AGC^GCT
Asp718I 900 GGTACC G^GTACC
AspI 502 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1146 TGCGCA TGC^GCA
BalI 650 TGGCCA TGG^CCA
BanI 900 GGTACC G^G[CT][GA]CC
BbvCI 1608 GCTGAGG CCTCAGC(-5/-2)
BsaI 1455 GGTCTC GGTCTC(1/5)
BseAI 1997 TCCGGA T^CCGGA
BsmBI 58 GAGACG CGTCTC(1/5)
Bsp1407I 865 TGTACA T^GTACA
BspEI 1997 TCCGGA T^CCGGA
BsrGI 865 TGTACA T^GTACA
BssHII 143 GCGCGC G^CGCGC
BstBI 356 TTCGAA TT^CGAA
BstUI 144 CGCG CG^CG
BstYI 1673 AGATCC [GA]^GATC[CT]
DraI 199 TTTAAA TTT^AAA
DraII 1795 AGGCCCC [GA]G^G[ACGT]CC[CT]
DrdI 60 GACGCTCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 12 AGCGCT AGC^GCT
EcoO109I 1795 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRV 1315 GATATC GAT^ATC
EcoT22I 1185 ATGCAT ATGCA^T
FspI 1146 TGCGCA TGC^GCA
HaeII 12 AGCGCT [GA]GCGC^[CT]
HindIII 1049 AAGCTT A^AGCTT
HpaI 681 GTTAAC GTT^AAC
KpnI 900 GGTACC GGTAC^C
MflI 1673 AGATCC [GA]^GATC[CT]
MluNI 650 TGGCCA TGG^CCA
MroI 1997 TCCGGA T^CCGGA
MscI 650 TGGCCA TGG^CCA
MslI 517 CATTTCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 144 CGCG CG^CG
NsiI 1185 ATGCAT ATGCA^T
NspV 356 TTCGAA TT^CGAA
PaeR7I 16 CTCGAG C^TCGAG
PflFI 502 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1191 CCATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1785 CTGCAG CTGCA^G
SacI 112 GAGCTC GAGCT^C
SexAI 503 ACCTGGT A^CC[AT]GGT
SfcI 1785 CTGCAG C^T[GA][CT]AG
SfuI 356 TTCGAA TT^CGAA
SpeI 1348 ACTAGT A^CTAGT
SspBI 865 TGTACA T^GTACA
StuI 1612 AGGCCT AGG^CCT
SwaI 198 ATTTAAAT ATTT^AAAT
Tth111I 502 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1191 CCATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 16 CTCGAG C^TCGAG
XhoII 1673 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 1021 GTAGAC GT^[AC][GT]AC
1108 GTCTAC
AlwI 1674 GATCC GGATC(4/5)
1211 GGATC
AocI 279 CCTCAGG CC^T[ACGT]AGG
1615 CCTCAGG
AvaI 16 CTCGAG C^[CT]CG[GA]G
1451 CTCGGG
BanII 4 GGGCTC G[GA]GC[CT]^C
112 GAGCTC
BcgI 1743 GCACCTGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2063 GCACGGTCTTCG
BcnI 1098 CCGGG CC^[GC]GG
1973 CCCGG
BfaI 725 CTAG C^TAG
1349 CTAG
BsaAI 817 TACGTG [CT]AC^GT[GA]
2152 TACGTG
BsaWI 1067 ACCGGA [AT]^CCGG[AT]
1997 TCCGGA
BsmI 318 GAATGC GAATGC(1/-1)
1373 GAATGC
BsoBI 16 CTCGAG C^[CT]CG[GA]G
1451 CTCGGG
BspHI 1391 TCATGA T^CATGA
1520 TCATGA
BstAPI 1260 GCAGAAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1534 GCAAATGTTGC
Bsu36I 279 CCTCAGG CC^T[ACGT]AGG
1615 CCTCAGG
EaeI 650 TGGCCA [CT]^GGCC[GA]
1095 TGGCCG
Eco81I 279 CCTCAGG CC^T[ACGT]AGG
1615 CCTCAGG
HgaI 60 GACGC GACGC(5/10)
1803 GACGC
MaeI 725 CTAG C^TAG
1349 CTAG
MspA1I 1513 CAGCTG C[AC]G^C[GT]G
1738 CAGCTG
NciI 1098 CCGGG CC^[GC]GG
1973 CCCGG
PvuII 1513 CAGCTG CAG^CTG
1738 CAGCTG
RcaI 1391 TCATGA T^CATGA
1520 TCATGA
SapI 1758 GAAGAGC GCTCTTC(1/4)
1806 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AfaI AhdI AluI
ApoI AspEI AspHI AvaII BbsI
BbvI BciVI BmyI BpmI BpuAI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DsaI Eam1105I EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MseI MspI MvaI MwoI NcoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I