Restriction map for
pF1KA0628
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
Acc65I AccI AccIII AciI AfaI
AflII AgeI AocI ApaI AscI
AseI AsnI Asp718I AvrII BbeI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BseAI
BseRI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstEII BstPI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI EclXI Eco52I
Eco81I EcoO65I EcoT14I EcoT22I FauI
FbaI FseI HgaI HincII HindII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MspA1I MunI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PflMI PinAI PmeI PpuMI
PshAI PshBI PstI PvuI PvuII
RcaI RsaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SpeI Sse8387I
SspBI SspI StyI SwaI Van91I
XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccII 595 CGCG CG^CG
AclI 1501 AACGTT AA^CGTT
AflIII 452 ACACGT A^C[GA][CT]GT
AhdI 530 GACTCTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 288 GTGCAC G^TGCAC
Aor51HI 1061 AGCGCT AGC^GCT
ApaLI 288 GTGCAC G^TGCAC
AspEI 530 GACTCTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 96 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 408 GGACC G^G[AT]CC
AviII 627 TGCGCA TGC^GCA
BalI 480 TGGCCA TGG^CCA
BamHI 1016 GGATCC G^GATCC
BanI 671 GGCACC G^G[CT][GA]CC
BbrPI 453 CACGTG CAC^GTG
BfaI 412 CTAG C^TAG
BsaJI 201 CCTGGG C^C[ACGT][ACGT]GG
BsaWI 940 ACCGGA [AT]^CCGG[AT]
BspMI 237 GCAGGT ACCTGC(4/8)
BssSI 154 CACGAG CACGAG(-5/-1)
BstBI 1291 TTCGAA TT^CGAA
BstUI 595 CGCG CG^CG
BstXI 1556 CCATACATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 480 TGGCCA [CT]^GGCC[GA]
Eam1105I 530 GACTCTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1447 GAAGAG CTCTTC(1/4)
Eco47III 1061 AGCGCT AGC^GCT
EcoNI 812 CCTTCATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 376 GATATC GAT^ATC
FspI 627 TGCGCA TGC^GCA
HaeII 1061 AGCGCT [GA]GCGC^[CT]
HapII 941 CCGG C^CGG
HindIII 1145 AAGCTT A^AGCTT
HpaII 941 CCGG C^CGG
Ksp632I 1447 GAAGAG CTCTTC(1/4)
MaeI 412 CTAG C^TAG
MluNI 480 TGGCCA TGG^CCA
MscI 480 TGGCCA TGG^CCA
MspI 941 CCGG C^CGG
MvnI 595 CGCG CG^CG
NlaIII 433 CATG CATG^
NspI 432 GCATGC [GA]CATG^[CT]
NspV 1291 TTCGAA TT^CGAA
PaeR7I 825 CTCGAG C^TCGAG
PflFI 96 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 453 CACGTG CAC^GTG
PmlI 453 CACGTG CAC^GTG
Psp1406I 1501 AACGTT AA^CGTT
SfcI 1577 CTGTAG C^T[GA][CT]AG
SfuI 1291 TTCGAA TT^CGAA
SnaBI 1039 TACGTA TAC^GTA
SphI 432 GCATGC GCATG^C
StuI 1033 AGGCCT AGG^CCT
Tth111I 96 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 825 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AatII 99 GACGTC GACGT^C
1074 GACGTC
AcyI 99 GACGTC G[GA]^CG[CT]C
1074 GACGTC
AlwNI 196 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
225 CAGGGGCTG
AvaI 136 CCCGAG C^[CT]CG[GA]G
825 CTCGAG
BbvI 230 GCTGC GCAGC(8/12)
1335 GCTGC
BsaAI 453 CACGTG [CT]AC^GT[GA]
1039 TACGTA
BsaHI 99 GACGTC G[GA]^CG[CT]C
1074 GACGTC
BsgI 1342 CTGCAC GTGCAG(16/14)
1380 GTGCAG
BsmFI 1280 GGGAC GGGAC(10/14)
1533 GGGAC
BsoBI 136 CCCGAG C^[CT]CG[GA]G
825 CTCGAG
BssKI 65 CCTGG ^CC[ACGT]GG
201 CCTGG
BstF5I 1320 CATCC GGATG(2/0)
78 GGATG
BstNI 65 CCTGG CC^[AT]GG
201 CCTGG
Cac8I 233 GCAAGC GC[ACGT]^[ACGT]GC
432 GCATGC
EcoRII 65 CCTGG ^CC[AT]GG
201 CCTGG
Fnu4HI 230 GCTGC GC^[ACGT]GC
1335 GCTGC
FokI 1320 CATCC GGATG(9/13)
78 GGATG
Hin1I 99 GACGTC G[GA]^CG[CT]C
1074 GACGTC
ItaI 230 GCTGC GC^[ACGT]GC
1335 GCTGC
MvaI 65 CCTGG CC^[AT]GG
201 CCTGG
ScrFI 65 CCTGG CC^[ACGT]GG
201 CCTGG
TfiI 25 GATTC G^A[AT]TC
765 GATTC
TseI 230 GCTGC G^C[AT]GC
1335 GCTGC
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# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AcsI AluI AlwI ApoI Asp700I
AspHI BanII BmyI BpmI BsaI
BsiHKAI BsiYI BslI BsmAI BsmI
Bsp1286I BsrDI BsrI BstYI CfoI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRI HaeIII HhaI HinP1I HinfI
HphI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MwoI
NdeII NlaIV PleI SacI Sau3AI
Sau96I SfaNI SmlI TaqI Tru9I
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI