Restriction map for
pF1KA0656
##################################
# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AflIII AhdI AocI AscI AseI
AsnI Asp700I AspEI AspI AvaI
AviII AvrII BalI BbrPI BcgI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BseAI BsiEI
BsiWI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII ClaI CpoI DraI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoO65I EcoRI FbaI FseI FspI
HindIII HpaI Ksp632I KspI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NdeI NgoMIV NotI
NruI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI StuI SwaI Tth111I
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 360 GTCTAC GT^[AC][GT]AC
AcyI 2034 GGCGCC G[GA]^CG[CT]C
AgeI 978 ACCGGT A^CCGGT
Alw44I 2432 GTGCAC G^TGCAC
Aor51HI 1945 AGCGCT AGC^GCT
ApaLI 2432 GTGCAC G^TGCAC
BamHI 340 GGATCC G^GATCC
BbeI 2034 GGCGCC GGCGC^C
BbsI 912 GTCTTC GAAGAC(2/6)
BcnI 2483 CCGGG CC^[GC]GG
BpuAI 912 GTCTTC GAAGAC(2/6)
BsaBI 544 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2034 GGCGCC G[GA]^CG[CT]C
BspHI 93 TCATGA T^CATGA
BssSI 734 CACGAG CACGAG(-5/-1)
BstXI 2401 CCAGCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 2426 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 732 GACACGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 176 TGGCCG [CT]^GGCC[GA]
Eco47III 1945 AGCGCT AGC^GCT
EcoRV 1684 GATATC GAT^ATC
HgaI 828 GACGC GACGC(5/10)
Hin1I 2034 GGCGCC G[GA]^CG[CT]C
HincII 2364 GTTGAC GT[CT]^[GA]AC
HindII 2364 GTTGAC GT[CT]^[GA]AC
KasI 2034 GGCGCC G^GCGCC
MaeII 934 ACGT A^CGT
MamI 544 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 2034 GGCGCC GG^CGCC
NciI 2483 CCGGG CC^[GC]GG
NheI 1534 GCTAGC G^CTAGC
PinAI 978 ACCGGT A^CCGGT
PpuMI 2684 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 93 TCATGA T^CATGA
SexAI 2407 ACCTGGT A^CC[AT]GGT
SfoI 2034 GGCGCC GGC^GCC
SmlI 751 CTCAAG C^T[CT][GA]AG
SphI 2543 GCATGC GCATG^C
TaqI 656 TCGA T^CGA
TthHB8I 656 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Acc65I 2220 GGTACC G^GTACC
2314 GGTACC
ApaI 105 GGGCCC GGGCC^C
2486 GGGCCC
Asp718I 2220 GGTACC G^GTACC
2314 GGTACC
AspHI 890 GTGCTC G[AT]GC[AT]^C
2432 GTGCAC
BanII 105 GGGCCC G[GA]GC[CT]^C
2486 GGGCCC
BbvCI 1259 CCTCAGC CCTCAGC(-5/-2)
2557 CCTCAGC
BglII 606 AGATCT A^GATCT
2334 AGATCT
BsaWI 978 ACCGGT [AT]^CCGG[AT]
1548 TCCGGT
BsiHKAI 890 GTGCTC G[AT]GC[AT]^C
2432 GTGCAC
BsmBI 935 CGTCTC CGTCTC(1/5)
826 GAGACG
BsmI 2346 GAATGC GAATGC(1/-1)
2522 GAATGC
BsrDI 1489 GCAATG GCAATG(2/0)
2236 GCAATG
BsrFI 978 ACCGGT [GA]^CCGG[CT]
2565 GCCGGT
Cfr10I 978 ACCGGT [GA]^CCGG[CT]
2565 GCCGGT
DsaI 345 CCATGG C^C[GA][CT]GG
2183 CCATGG
FauI 1555 CCCGC CCCGC(4/6)
195 GCGGG
KpnI 2220 GGTACC GGTAC^C
2314 GGTACC
NcoI 345 CCATGG C^CATGG
2183 CCATGG
NspI 1417 GCATGT [GA]CATG^[CT]
2543 GCATGC
PstI 1355 CTGCAG CTGCA^G
1724 CTGCAG
PvuII 1589 CAGCTG CAG^CTG
2192 CAGCTG
SspI 163 AATATT AAT^ATT
284 AATATT
XcmI 812 CCAGCAGTCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2228 CCAGTCCTGCAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BanI BbvI
BfaI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrBI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoNI EcoO109I EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NsiI PflMI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XbaI
XhoII